Mafg (NM_010756) Mouse Untagged Clone

SKU
MC207324
Mafg (untagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein G (avian) (Mafg), (10ug)
  $165.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Mafg
Synonyms AA545192; C630022N07Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC207324 representing NM_010756
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGACCCCCAATAAAGGAAACAAGGCCTTAAAGGTGAAGCGGGAGCCAGGCGAGAATGGCACCAGCT
TGACCGACGAGGAGCTGGTAACCATGTCGGTGCGAGAGTTGAACCAGCACCTGCGAGGCCTCTCCAAGGA
AGAGATCATCCAGCTGAAGCAGCGGAGGCGCACACTGAAGAACCGGGGCTACGCGGCCAGCTGCCGCGTC
AAGCGGGTGACACAGAAGGAGGAGCTGGAGAAGCAGAAGGCGGAGCTCCAGCAGGAGGTGGAGAAGCTGG
CCTCGGAGAATGCCAGCATGAAGCTGGAGCTCGATGCCCTGCGCTCCAAGTACGAGGCCCTGCAGAACTT
TGCCAGGACCGTGGCCCGCAGCCCTGTGGCCCCAGCTCGGGGTCCCCTTGCTGCTGGCCTGGGGCCCCTG
GTTCCTGGCAAGGTGGCTGCCACCAGCGTCATCACAATAGTAAAGTCCAAGACGGATGCTCGGTCATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_010756
Insert Size 489 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010756.3, NP_034886.1
RefSeq Size 1720 bp
RefSeq ORF 489 bp
Locus ID 17134
UniProt ID O54790
Cytogenetics 11 84.35 cM
Summary Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:16738329, PubMed:9679061). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1 and NFE2L2, and recruiting them to specific DNA-binding sites (PubMed:16738329, PubMed:9679061). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NFE2L2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NFE2L2. Activates globin gene expression when associated with NFE2L2 (By similarity). May be involved in signal transduction of extracellular H(+) (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010756" in other vectors (3)
SKU Description Size Price
MR201284 Mafg (Myc-DDK-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein G (avian) (Mafg) 10 ug
$289.00
MR201284L3 Lenti ORF clone of Mafg (Myc-DDK-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein G (avian) (Mafg) 10 ug
$450.00
MR201284L4 Lenti ORF clone of Mafg (mGFP-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein G (avian) (Mafg) 10 ug
$450.00

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