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Arhgap24 (NM_146161) Mouse Untagged Clone

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SKU
MC205804
Arhgap24 (untagged) - Mouse Rho GTPase activating protein 24 (Arhgap24), transcript variant 2, (10ug)
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ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $610.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Arhgap24
Synonyms 0610025G21Rik
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC023344
GAGACGTGGTTTCCATTCCTGGGTGATGGAGTCATGGGAGAAACTGCATTGAGCAAACGAAGTGGGACCT GAGCCGGCTCCAGTGTTCACTAAGCCGCTCAGGGCAGAGCAGCTGCTGTCTCTCCCAGCCTGCATCTGTC ACTGACCACTGAAGTGTGTGTGCAAGGAGGTGAACGAGATCGGATGACAGCCAATCATGAGAGCTATCTC CTCATGGCGAGCACCCAGAATGACATGGAAGACTGGGTGAAGTCCATCCGCCGAGTCATATGGGGACCTT TTGGAGGAGGTATTTTTGGACAGAAACTGGAAGACACAGTCCGATATGAAAAGAGGTACGGGAACCGGCT GGCACCCATGCTGGTGGAGCAGTGTGTGGACTTCATCCGACAGCGGGGGCTGAAGGAAGAAGGCCTCTTC CGGCTGCCGGGCCAGGCGAACCTGGTCAAGGAGCTCCAGGATGCGTTCGACTGTGGAGAGAAGCCCTCCT TCGACAGCAACACCGACGTCCACACTGTGGCATCCCTGCTGAAATTGTATCTCCGAGAACTTCCAGAGCC CGTCGTTCCTTATGCAAAGTATGAAGACTTTTTGTCCTGCGCCACGCTGCTCAGTAAGGAGGAAGAAGCA GGTGTTAAGGAGCTAATGAAACAAGTGAAGAGTTTGCCTGTGGTTAACTACAACCTTCTGAAGTACATTT GCAGGTTCCTGGATGAAGTGCAGTCCTATTCAGGAGTTAACAAGATGAGCGCACAGAACTTGGCAACTGT CTTCGGTCCTAACATCCTGCGCCCAAAGGTGGAAGATCCCTTGACCATCATGGAGGGCACTGTGGTGGTC CAGCAGTTAATGTCAGTGATGATTAGTAAGCATGATCGACTCTTCCCCAAAGACACAGAACCACAAAGCA AGCCCCAAGACGGCCCGAACAGCAATAATAACGATGGTCATAAGAAAGCCACCATGGGTCAGTTGCAGAA CAAAGAAAACAATAATACCAAGGAGAGCCCCGTCAGAAGGTGCTCTTGGGACAAGCCTGAATCCCCCCAG AGAAGCAGCGTGGACAATGGATCCCCCACAGCCCTATCAGGCAGCAAAACCAACAGCCCAAGGAACAGTA TCCACAAGCTGGATATTTCCAGGAGCCCCCCTCTCATGGTCAAAAAGAACCCAGCCTTCAACAAGGGCAG TGGGATTGTCACCAACGGGTCATTCAGCAGCAGCAATGCAGAAGGTGTCGAGAAGCCTCAAACTACTCCT AATGGGAGTTTACAAGCCAGAAGGACCTCTTCACTGAAGAGTTCTGGTACCAAAATGGGCACTCACAGTG TACAGAATGGCACTGTCCGCATGGGTATTCTGAACACTGACACACTAGGGAATTCCTTGAACGGCCGGAG CATGAGTTGGCTGCCCAATGGCTATGTGACCCTGAGAGATAACAAACAGAAGGAGCCAGCAGGAGAGTCA GGCCAGCACAACAGACTCTCCACCTACGACAATGTCCACCAGCAGTTCTCCTCGATGAGCCTCGACGACA AGCACAGCGTGGACAGTGCCACGTGGTCCACCTCCTCCTGCGAAATCTCCCTCCCTGAGAACTCCAATTC CTGCCGCTCCTCTACCACCACGTGCCCAGAGCAAGACTTCTACGTTGGGAACTTTGAGGACCCTGTTTTA GACGGCCCACCTCAGGATGATCTTTCCCATCCCGGGGACTATGAGAACAAAAGTGACCGCAGGAGCGTAG GGGGCCGAAGCAGCCGCGCCACGAGCAGCAGCGATAACAGCGAGACATTCGTGGGCAACACCAGCAGCAA CCACAGTGCACTTCACAGTTTAGTTTCCAGCCTGAAGCAGGAGATGACCAAACAGAAGATCGAGTATGAG TCCAGGATAAAAAGCTTAGAACAGCGGAATTTGACTTTGGAAACGGAAATGCTGAGCCTCCATGACGAAC TGGATCAAGAGAGGAAAAAGTTCACCATGATAGAAATCAAGATGAGAAACGCTGAGCGCGCAAAGGAGGA TGCGGAGAAAAGGAACGACATGCTCCAGAAGGAGATGGAGCAGTTCTTCTCCACATTTGGAGACCTGACA GTGGAACCCAGGAGAAGCGAGAGAGGAAACACAATCTGGATCCAGTGAGCCTTTCTCTGTCCTGGACCGC TCGCTCCGCGGAGGACTCTGGGGACTCCGGGGCAAACAGTGTTGGGGAACAGGTGTGGGATCAGGTGGTT GGTCTGTGTAGAGCTTCATCTGGTGCAGGAATCTCATTTCCAGACATCACTATCCATATCTGCAGTGTGT ACCAAAGTTATATCATGCTCCATAATGCTACTGTCAAGTGTTACAACTGGATATGTGTATAGAGAGTAGT TTCTAAGATGTCAATAAGAGTAAGAAGCATATATCTCAATGATTATTTTATTGCAAGTCCTGTATTTAAA TGTCGAATCAATACTTTGTTGCAATTTAGCTTGCATTCAAGCTTCACCCCGTGCACTTAACATAAGCTAT TTTTGGCATCGTGTTATCATCTGCTTATTTTATAGATCAATATTTTTATTTCCCCTTTTGTCGAGGAAAT GGAGATAAGAAGAGATATAAATATATAAATATATATAAGATGAGTTATTAAAGTCAAAAGAATACTTTGT GGCTGTGCTGTTTGTGCCAATAGACCTTGCCATGACCAAAAAAGAGCAATGTAAAACAAAAAAAAAAAAA AA
Restriction Sites RsrII-NotI |
ACCN NM_146161
Insert Size 1965 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC023344, AAH23344
RefSeq Size 2732 bp
RefSeq ORF 1965 bp
Locus ID 231532
UniProt ID Q8C4V1
Cytogenetics 5 E5
Summary Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which has a shorter N-terminus, compared to isoform 1.
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Documents
Resources
"NM_146161" in other vectors (3)
SKU Description Size Price
MR209784 Arhgap24 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 24 (Arhgap24), transcript variant 2 10 ug
$289.00 $610.00
MR209784L3 Lenti ORF clone of Arhgap24 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 24 (Arhgap24), transcript variant 2 10 ug
$910.00
MR209784L4 Lenti ORF clone of Arhgap24 (mGFP-tagged) - Mouse Rho GTPase activating protein 24 (Arhgap24), transcript variant 2 10 ug
$910.00

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