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Fig4 (NM_133999) Mouse Untagged Clone

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SKU
MC205750
Fig4 (untagged) - Mouse FIG4 homolog (S. cerevisiae) (Fig4), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $831.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Fig4
Synonyms A530089I17Rik; AI326867; Sac3
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC015295
GGGAATCCGGAAGCAGCCGGCGAGCCCGCTGCTGAACGTTTGGTGTTCTGACACTGTTTTTGTCAGCTGT GTCTGGTGTGCTGGAGGTCTGCGGGGTCTCCTCGCTCTGTCTCCAGGGGGAACCCCGCCGCTGTCACGAC CTGGGCCGCGGCCTGGTGCCCTGTGGCGGGGCCGCCATTGGCTGGAGCCATGCCCACGGCCGCTGCCCCC ATCATCAGCTCGGTCCAGAAGCTGGTGCTGTATGAGACCAGAGCTAGATACTTTCTAGTTGGGAGCAATC ATGCAGAAACGAAGTACCGTGTCCTGAAAATTGATAGAACAGAACCGAAGGACTTGGTGGTAATCGACGA CAGGCACGTGTACACACAACAAGAAGTGAGGGAACTTCTTGGCCGCCTGGATCTGGGAAACAGAACAAAG ATGAGCCAGAAAGGATCCTCCGGGTTGTTTCGAGCCGTCTCAGCTTTTGGAGTCGTAGGTTTTGTCAGGT TCTTAGAAGGCTACTATATTGTATTAATAACTAAGCGGAGGAAGATGGCAGACATTGGAGGTCATGCAAT TTATAAGATTGAAGATACAAGTATGATTTATATCCCCAATGACTCTGTTCGGATTTCTCACCCCGACGAA GCTAGGTATCTAAGGATATTTCAAAATGTGGATCTATCTAGCAATTTTTACTTTAGTTACAGCTATGATT TGTCACACTCACTTCAGTATAATCTTACCGTCCTGCGAATGCCCCTGGAGATGTTAAAGTCAGAAACATC CAAGGCGTGCCAGGAGAGCTTCGACATCTTTGAAGATGAAGGATTAATTACACAGGGTGGCAGTGGTGTA TTTGGGATCTCTAGTGAGCCTTATATGAAGTATGTATGGAACGGTGAACTTCTGGATATAATTAAAAACA CTGTGCATCGTGACTGGCTATTGTATATCATTCATGGGTTCTGCGGGCAGTCCAAGCTTTTGATCTATGG ACGACCAGTGTATGTGACCCTAATAGCCAGGAGATCCAGTAGGTTTGCTGGGACGCGCTTTCTCAAGAGA GGCGCAAACTGTGAGGGTGACGTTGCAAATGAGGTGGAGACCGAGCAGATCCTCTGTGATGCTTCTGTGA TGTCTTTTACCGCAGGCAGTTACTCTTCTTACGTACAAGTTAGAGGATCCGTTCCCTTATTCTGGTCCCA AGACATCTCAACTATGATGCCGAAACCACCCATTACACTGGACCAGGCGGATCCCTTTGCACACGTGGCT GCTCTTCACTTTGACCAGATGCTCCAGAGGTTCGGCTCCCCCATCATCATCTTGAACTTAGTGAAGGAGA GGGAGAAGAGAAAGCACGAAAGGATCCTGAGTGAAGAGCTGGTGGCTGCGGTCACTTACCTGAACCAGTT CCTGCCACCGGAGCACACCATTGTCTACATCCCCTGGGACATGGCCAAGTACACCAAGAGTAAGCTGTGC AACGTTCTCGATCGGCTGAATGTGATTGCGGAAAGCGTGGTAAAGAAGACAGGATTCTTTGTAAACCGCC CTGATTCTTACTGCAGCATTTTACGGCCAGATGAAAAGTGGAATGAACTAGGAGGTCATGTGATTCCCAC TGGTCGGCTCCAGACTGGCATCCTTCGAACCAACTGTGTGGACTGTTTGGATCGCACCAACACTGCGCAG TTCATGGTGGGCAAGTGTGCCCTGGCTTACCAGCTGTATTCCTTAGGGCTGATTGACAAGCCTAACCTGC AGTTCGACACGGATGCAGTGAGGTTGTTTGAGGAACTCTATGAAGACCATGGAGACACCCTTTCCCTGCA GTACGGAGGCTCTCAGCTGGTTCATCGGGTAAAGACCTACAGAAAGATAGCACCGTGGACCCAGCACTCC AAGGACATCATGCAGACTCTGTCCCGGTACTACAGCAACGCCTTCTCGGATGCTGATAGACAAGATTCCA TCAACCTGTTTTTGGGTGTTTTCCACCCCACAGAAGGGAAACCTCATCTCTGGGAACTCCCCACAGACTT TTACCTCCATCACAAAAACACCATGAGCCTTTTACCCCCCAGAAGGAGTTATACTTACTGGTGGACGCCA GAGGTGGTGAAGCATCTGCCGCTGCCTTACGATGAAGTTATCTGTGCTGCAAACTTAAAGAAGTTGATGG TGAAGAAATTTCACAGATGGGAAGAAGAGATTGATATTCACAATGAGTTCTTCCGGCCGTATGAATTGAG TAGTTTTGATGACACCTTTTGCTTGGCCATGACAAGTTCAGCACGTGATTTTATGCCTAAAACTGTTGGC ATTGATCCAAGTCCATTTACCGTGCGAAAACCAGATGAAACTGGAAAATCTGTACTGGGGAACAAAAACA CTAGAGAGGAAGCTGTCCTGCAGCGGAAGACGGCAGCCAGCGCCCCGCCACCCCCGAGCGAGGAGGCTGT GTCCAGCAGCTCTGAGGATGACTCTGGCACCGACCGGGAAGATGAAGGCTCCATCTCTCAGCGATCCACT CCTGTGAAGATGACTGACACGGGAGACAGTGCCAAAGCGACTGAGAATGTGGTCCAGCCCATGAAGGAAG TGTATGGAGTCAGTCTCTCCAGTAGCCTGTCAGAGGAGGATCACTCCATTTATGCCAGGTTTGTTCAGCT GGGGCAAAGTCAACATAAACAGGACCGAGGTAACCAGCAGCTGTGTTCCCGTTGCTCAGATGGAGTTATA AAACTAACTCCCATCTCAGCTTTCTCGCAAGACAACATTTATGAAGTTCAGCCTCCAAGAGTAGACAGAA AGTCTACAGAGATCTTCCAGGCCCATATCCAAGCTAGCCAAGGTATCATGCAGCCCCTTGGCAAAGAGGA CACTGCCATGTACCGGGAGTACATCAGAAACCGCTACTTATAGAGACGGCCTCGAGGACAGTGTCCTCTG TGAGAAGGCGTGCCCCAGATCCTGAGATGCAGCTCATTGGTAAAGGACTCCCCAGCACCTGTATCACAGC CTTCGGTGATTCCAGCTACCAGGAATCCAGTGTTGTCTTCCAGATTTTGTAGGCACCTATACTCAAGTAG GTGCCTGTGTGTGCACACACATGCACAGGAGTGCATACACACACATGTATGCACACACACAGTAAAAATA ATAAAAGTAAAATCTTTAAAAATCAATTCATCCAAATCATGTATCTGAAACTTTTCACCTCTTTGTACCA TGCAAATGACTAATTGCAGGTAATAAAGAAATGCTGCCTCTTTAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_133999
Insert Size 2724 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC015295, AAH15295
RefSeq Size 3278 bp
RefSeq ORF 2724 bp
Locus ID 103199
UniProt ID Q91WF7
Cytogenetics 10 B1
Summary The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide substrates in the order PtdIns(4,5)P2 > PtdIns(3,5)P2 > PtdIns(3,4,5)P3. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133999" in other vectors (4)
SKU Description Size Price
MG211140 Fig4 (tGFP-tagged) - Mouse RIKEN cDNA A530089I17 gene (A530089I17Rik) 10 ug
$1,447.00
MR211140 Fig4 (Myc-DDK-tagged) - Mouse FIG4 homolog (S. cerevisiae) (Fig4) 10 ug
$1,247.00
MR211140L3 Lenti ORF clone of Fig4 (Myc-DDK-tagged) - Mouse FIG4 homolog (S. cerevisiae) (Fig4) 10 ug
$1,547.00
MR211140L4 Lenti ORF clone of Fig4 (mGFP-tagged) - Mouse FIG4 homolog (S. cerevisiae) (Fig4) 10 ug
$1,547.00

file-alt Citations

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