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Chaf1b (NM_028083) Mouse Untagged Clone

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SKU
MC205728
Chaf1b (untagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $533.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Chaf1b
Synonyms 2600017H24Rik; C76145; CAF-I p60; CAF-Ip60; CAF1; CAF1A; CAF1P60; MPHOSPH7
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC013532
CGGAAACTCTGGAGGATGAAAGTGATCACGTGTGAAATCGCCTGGCACAACAAGGAGCCAGTGTACAGCC TGGACTTCCAGCATGGGGCAACCTGGAAGATCCACAGGCTGGCATCCGCGGGGGTGGACACCGCCGTCAG GATCTGGAAGTTGGAGAGGGGGCCAGATGGGAAAGCCATTGTGGAATTTCTCTCCAATCTTGCTCGACAC ACCAAAGCTGTCAATGTTGTACGCTTTTCTCCAACTGGGGAGATTTTAGCATCTGGAGGAGATGATGCCG TCATTCTGTTGTGGAAGATGAATGACAGCAAGGAGCCGGAGCAGATCGCCTTTCAGGATGAGGAGGAGGC TCAGCTGAATAAGGAGAACTGGACTGTGGTAAAGACCCTGAGGGGCCACTTAGAAGATGTGTATGATATT TGCTGGGCAACCGATGGGAATTTGATGACCTCTGCCTCTGTGGATAACACAGTTATCATATGGGATGTCA GTAAAGGGCAGAAAATTTCCATTTTCAACGAGCATAAAAGTTATGTGCAAGGAGTCACCTGGGATCCTTT GGGTCAATATATTGCGACCCTGAGCTGTGACAGGGTGCTGCGGATCTATAATACCCAGAAAAAGCGTGTG GCTTTCAACATTTCAAAGATGCTCTCTGGACAGGGGCCTGAAGGAGAGGCAAGAAGTTTCCGGATGTTTC ACGACGACAGCATGAAGTCGTTCTTCCGAAGGCTCAGCTTTACTCCAGATGGATCTTTGCTCCTCACTCC AGCCGGATGTATGGAATCTGGTGAGAATGTGACAAACACCACTTATGTGTTCTCCAGAAAACACCTGAAA AGGCCAATCGCCCATCTTCCGTGCCCTGGGAAAGCGACTCTTGCTGTCCGCTGTTGTCCTGTCTACTTTG AATTGAGGCCAGTGGCAGAAACAGAGAAAGCATCAGAGGAGCCCAGCCCCGAGCTGGTGAACCTGCCCTA CCGTATGGTGTTCGCGGTGGCTTCCGAGGACTCTGTGTTGCTCTATGACACTCAACAGTCTTTCCCATTT GGCTATGTGTCTAACATCCATTACCATACCCTGAGTGACATTTCCTGGTCCAGCGATGGGGCCTTCTTGG CCATTTCCTCCACGGATGGCTATTGCACATTTGTGACCTTCGAGAAAGGTGAACTTGGCATACCTTTGAA AGAGAAGCCAGTGCTGAGCATAAGGACTCCCGACACAGCCAAGAAAGCCAAGAACCAGACACACCAGGGA TCTTCGCCGGGATCCAGATCAGTCGAAGGGACCCCCAGCAACAGAACCCAAGACCCCAGCAGTCCCTGTA CCACTCCTTCACCAACCACACAGTCTCCAGCTCCGTCGGCCATCAAGGACAGTCCCTCAGCCATCCCGGC TGGCAAAAGCCCCTTGCCACAACCTTCTGAAGAGAAGACCCTGCAACCTGCTGGGCAAAACATGAAAGCC CCCCAACCCCGCAGGGTCACCCTGAACACGCTGCAGACTTGGGGCAAGACAGCCCCCCGGAGAATAAATT TAACACCATTGAAAACAGACACTGTGCCAAATCCACAGCCCAATAGCGGGACCGCCCCCTCCACAGAAGA GGTCCAGCCAGAGGCTCCTGGGGAGCCACCGGAGGAGCCTCCTGAACTGAAGAGACCCAGGCTTGAGGAA CGTGAAGGAGATGCCCAAAACCTGGCCCCTGATGACTCTTCCAAGACTGTCTGAGCCTGCTATGCACCTG CGTGCAGAGGAGGCAAGGCCAAAGCTCGAAGGCTGGTGATGGTGGCCAAATAAACACTCATGAATTGGTT CACATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Restriction Sites RsrII-NotI |
ACCN NM_028083
Insert Size 1719 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC013532, AAH13532
RefSeq Size 1893 bp
RefSeq ORF 1719 bp
Locus ID 110749
UniProt ID Q9D0N7
Cytogenetics 16 54.96 cM
Summary Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer (By similarity).UniProtKB/Swiss-Prot Function
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"NM_028083" in other vectors (4)
SKU Description Size Price
MG209007 Chaf1b (tGFP-tagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b) 10 ug
$500.00
MR209007 Chaf1b (Myc-DDK-tagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b) 10 ug
$289.00 $300.00
MR209007L3 Lenti ORF clone of Chaf1b (Myc-DDK-tagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b) 10 ug
$600.00
MR209007L4 Lenti ORF clone of Chaf1b (mGFP-tagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b) 10 ug
$600.00

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