Wnt1 (NM_021279) Mouse Untagged Clone

SKU MC205633

Wnt1 (untagged) - Mouse wingless-related MMTV integration site 1 (Wnt1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Wnt1
• Synonyms: Int-1; sw; swaying; Wnt-1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC005449; GCCACAGCTTCGCTCGCCACTCATTGTCTGTGGCCCTGACCAGTGCGCCCTGGTGCTTTTAGTGCCGCCC GGGCCCGGAGGGGCAGCCTCTTCTCACTGCAGTCAGCGCCGCAACTATAAGAGGCGGTGCCTCCCGCAGT GGCTGCTTCAGCCCAGCAGCCAGGACAGCGAACCATGCTGCCTGCGGCCCGCCTCCAGACTTATTAGAGC CAGCCTGGGAACTCGCATCACTGCCCTCACCGCTGTGTCCAGTCCCACCGTCGCGGACAGCAACCACAGT CGTCAGAACCCCAGCACAGAACCAGCAAGGCCAGGCAGGCCATGGGGCTCTGGGCGCTGCTGCCCAGCTG GGTTTCTACTACGTTGCTACTGGCACTGACCGCTCTGCCCGCAGCCCTAGCTGCCAACAGTAGTGGCCGA TGGTGGGGCATCGTGAACATAGCCTCCTCCACGAACCTGTTGACGGATTCCAAGAGCCTGCAGCTGGTGC TCGAGCCCAGTCTGCAGCTGCTGAGCCGCAAGCAGCGGCGACTGATCCGACAGAACCCGGGGATCCTGCA CAGCGTGAGTGGAGGGCTCCAGAGCGCCGTGCGAGAGTGCAAATGGCAATTCCGAAACCGCCGCTGGAAC TGCCCCACTGCTCCGGGGCCCCACCTCTTCGGCAAGATCGTCAACCGAGGCTGCCGAGAAACAGCGTTCA TCTTCGCAATCACCTCCGCCGGGGTCACACATTCCGTGGCGCGCTCCTGCTCCGAAGGCTCCATCGAGTC CTGCACCTGCGACTACCGGCGGCGCGGCCCTGGGGGCCCCGACTGGCACTGGGGGGGCTGCAGTGACAAC ATCGATTTTGGTCGCCTCTTTGGCCGAGAGTTCGTGGACTCCGGGGAGAAGGGGCGGGACCTACGCTTCC TCATGAACCTTCACAACAACGAGGCAGGGCGAACGACCGTGTTCTCTGAGATGCGCCAAGAGTGCAAATG CCACGGGATGTCCGGCTCCTGCACGGTGCGCACGTGTTGGATGCGGCTGCCCACGCTGCGCGCTGTGGGC GACGTGCTGCGCGACCGCTTCGACGGTGCCTCCCGCGTCCTTTACGGCAACAGAGGCAGCAACCGCGCCT CGCGGGCGGAGCTGCTGCGCCTGGAGCCCGAAGACCCCGCGCACAAGCCTCCCTCCCCTCACGACCTCGT CTACTTCGAGAAATCGCCCAACTTCTGCACGTACAGTGGCCGCCTGGGCACAGCTGGCACAGCTGGACGA GCTTGCAACAGCTCGTCTCCCGCGCTGGACGGCTGTGAGCTGCTGTGCTGTGGCCGAGGCCACCGCACGC GCACGCAGCGCGTCACGGAGCGCTGCAACTGCACCTTCCACTGGTGCTGCCACGTCAGCTGCCGCAACTG CACGCACACGCGCGTTCTGCACGAGTGTCTATGAGGTGCCGCGCCTCCGGGAACGGGAACGCTCTCTTCC AGTTCTCAGACACACTCACTGGTCCTGATGTTTGCCCACCCTACCGCGTCCAGCCACAGTCCCAGGGTTC ATAGCGATCCATCTCTCCCACCTCCTACCTGGGGACTCCTGAAACCACTTGCCTGAGTCGGCTCGAACCC TTTTGCCATCCTGAGGGCCCTGACCCAGCCTACCTCCCTCCCTCTTTGAGGGAGACTCCTTTTGCACTGC CCCCCAATTTGGCCAGAGGGTGAGAGAAAGATTCTTCTTCTGGGGTGGGGGTGGGGAGGTCAACTCTTGA AGGTGTTGCGGTTCCTGATGTATTTTGCGCTGTGACCTCTTTGGGTATTATCACCTTTCCTTGTCTCTCA GGTCCCTATAGGTCCCTTGAGTTCTCTAACCAGCACCTCTGGGCTTCAAGGCCTTTCCCCTCCCACCTGT AGCTGAAGAGTTTCCGAGTTGAAAGGGCACGGAAAGCTAAGTGGGAAAGGAGGTTGCTGGACCCAGCAGC AAAACCCTACATTCTCCTTGTCTCTGCCTCGGAGCCATTGAACAGCTGTGAACCATGCCTCCCTCAGCCT CCTCCCACCCCTTCCTGTCCTGCCTCCTCATCACTGTGTAAATAATTTGCACCGAAATGTGGCCGCAGAG CCACGCGTTCGGTTATGTAAATAAAACTATTTATTGTGCTGGGTTCCAGCCTGGGTTGCAGAGACCACCC TCACCCCACCTCACTGCTCCTCTGTTCTGCTTGCCAGTCCTTTTGTTATCCGACCTTTTTTCTCTTTTAC CCAGCTTCTCATAGGCGCCCTTGCCCACCGGATCAGTATTTCCTTCCACTGTAGCTATTAGTGGCTCCTC GCCCCCACCAATGTAGTATCTTCCTCTGAGGAATAAAATATCTATTTTTAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_021279
• Insert Size: 1113 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC005449, AAH05449
• RefSeq Size: 2463 bp
• RefSeq ORF: 1113 bp
• Locus ID: 22408
• UniProt ID: P04426
• Cytogenetics: 15 54.65 cM
• Summary: Ligand for members of the frizzled family of seven transmembrane receptors. Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (By similarity). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (PubMed:15454084, PubMed:16116452). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (PubMed:2202907, PubMed:16116452). Has a role in osteoblast function, bone development and bone homeostasis (By similarity).[UniProtKB/Swiss-Prot Function]