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Rtcb (NM_145422) Mouse Untagged Clone

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SKU
MC205587
D10Wsu52e (untagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $471.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Rtcb
Synonyms AI255213; AI463255; D10Wsu52e; FAAP; HSPC117
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC003288
CCCACGCGTCCGGCGTGGAGCGGGTGGACTGCGGGGTGGTTTCTCTTTCTAAGCGTAGCCAGGAGCTATC GCTGGCCTCGCCGCCACCATGAGTCGTAACTACAACGATGAGCTACAGTTCTTGGACAAGATCAATAAAA ACTGCTGGAGGATCAAGAAGGGCTTTGTGCCCAACATGCAGGTTGAAGGAGTGTTTTATGTGAATGATGC TCTGGAAAAACTAATGTTTGAGGAATTAAGGAACGCCTGTCGAGGTGGTGGTGTTGGTGGCTTTCTGCCA GCCATGAAGCAGATTGGCAATGTGGCAGCCCTGCCTGGAATAGTTCATCGGTCTATCGGGCTTCCTGATG TCCATTCAGGCTATGGGTTTGCCATAGGGAACATGGCTGCCTTTGATATGAATGACCCTGAGGCCGTTGT ATCCCCAGGTGGTGTCGGATTTGATATTAACTGTGGTGTCCGCTTGCTAAGAACCAATTTAGATGAGAGC GATGTACAGCCTGTGAAGGAACAACTTGCCCAAGCTATGTTTGACCACATCCCTGTTGGGGTGGGATCAA AAGGTGTCATTCCAATGAATGCCAAAGACTTGGAGGAGGCATTGGAGATGGGGGTGGACTGGTCCCTGAG GGAAGGCTATGCCTGGGCTGAAGACAAGGAGCACTGTGAGGAGTATGGAAGGATGCTGCAAGCCGACCCC AATAAGGTCTCACCCAGGGCAAAGAAAAGGGGCCTTCCTCAGTTGGGGACCCTGGGAGCAGGCAACCATT ATGCAGAAATCCAGGTTGTAGATGAGATTTTCAATGAGTATGCCGCCAAGAAGATGGGCATCGACCATAA GGGACAGGTGTGTGTGATGATCCACAGTGGAAGCAGAGGCTTGGGCCACCAAGTAGCTACAGATGCACTG GTAGCTATGGAAAAGGCCATGAAGAGAGACAAGATTATAGTCAATGACCGGCAGTTGGCTTGTGCTCGGA TTGCATCCCCAGAGGGACAAGACTATCTAAAGGGAATGGCTGCAGCTGGAAACTACGCCTGGGTTAACCG CTCCTCTATGACCTTCTTAACCCGTCAGGCTTTTGCCAAAGTCTTCAACACAACCCCTGATGACCTGGAC CTGCATGTGATCTATGATGTGTCGCACAATATCGCCAAAGTGGAGCAGCACGTGGTGGATGGGAAGGAAC GGACGCTGCTGGTGCACAGGAAGGGATCCACCCGTGCTTTCCCGCCTCACCACCCCCTCATTGCTGTGGA TTATCAACTCACAGGACAACCAGTGCTTATTGGTGGCACCATGGGGACCTGTAGTTACGTTCTGACTGGC ACTGAACAAGGCATGACTGAGACCTTTGGAACAACCTGTCATGGAGCGGGCCGTGCTTTGTCCAGAGCAA AATCACGTCGTAACTTAGATTTCCAAGATGTCTTAGACAAACTGGCAGACATGGGAATTGCAATCCGGGT TGCTTCCCCCAAGCTGGTTATGGAAGAGGCACCAGAGTCCTATAAGAATGTGACAGACGTCGTGAACACC TGCCATGATGCTGGGATCAGCAAGAAGGCCATTAAACTGAGACCAATTGCTGTTATTAAAGGGTAGATCC AAGACTATCAGAGCGTCTGAGGTGCCCATGGACTGAAATTCCTAAGACATGAGACTCAAGGCCTGACAAG ATCCGAATTGCAGAGCTGTTGCTGTGCGTGCCACCATGGCTGGTGGAGAGGCTGCTGTCACAGGGTTGGA AAATAGCCTTCCAGGAGATGTGTCTCTGATGGGAGCAGGGCAAATGAGCCTCTTCTTCCTTTGTCCCACA GGGGTTTGTGACTGTCAATTGGGGTGTGGGAAGGGGCCCAGAAATGCCCTGTTTGGTCATACAAGTCTGT AATCACCAGAGTGGGCTTCTTTATCCCAGGCCCTGTTTGTAGCCCAGAAGGTATCATGGAGAACATGCTA TTAAATAGGTTTTTGAATGTTAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_145422
Insert Size 1518 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC003288, AAH03288
RefSeq Size 2003 bp
RefSeq ORF 1518 bp
Locus ID 28088
UniProt ID Q99LF4
Cytogenetics 10 41.81 cM
Summary Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs (By similarity). Essential during post-implantation development of embryos.UniProtKB/Swiss-Prot Function
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"NM_145422" in other vectors (4)
SKU Description Size Price
MG208125 D10Wsu52e (tGFP-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e) 10 ug
$671.00
MR208125 D10Wsu52e (Myc-DDK-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e) 10 ug
$289.00 $471.00
MR208125L3 Lenti ORF clone of D10Wsu52e (Myc-DDK-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e) 10 ug
$771.00
MR208125L4 Lenti ORF clone of D10Wsu52e (mGFP-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e) 10 ug
$771.00

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