Atf4 (NM_009716) Mouse Untagged Clone

SKU MC205262

Atf4 (untagged) - Mouse activating transcription factor 4 (Atf4), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Atf4
• Synonyms: Atf-4; C/ATF; CREB2; TAXREB67
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC085169; CTTTTCTCTCATGGGGCCTTTAGGACGATCTCTAACGCCACAGTTACCCCCCGAGCACAGCGGAGAAGGG TTGGGGCGGGCGAGGGCGTCAGTGAGAGGCGGTTTGGTGGCGCTGCGGTAGGATCACGTGACCACAGTGG GAGGATACGCGGTGTGCTGCGTCCCTGGCCGAGGCTATAAAGGGCGGGTTTAGGGCGTGCCGCCGCCATT TCTGCTTGCTGTCTGCCGGTTTAAGTTGTGTGCTCGGGTGTCCCTTTCCTCTTCCCCTCCCGCAGGGCTT GCGGCCACCATGGCGTATTAGAGGCAGCAGTGCCTGCGGCAGCGTTGGCCTTTGCAGCGGCGGCAGCAGC ACCAGGCTCTGCAGCGGCAACCCCCACCGGCCTAAGCCATGGCGCTCTTCACGAAATCCAGCAGCAGTGT TGCTGTAACGGACAAAGATACCTTCGAGTTAAGCACATTCCTCGAATCCAGCAAAGCCCCACAACATGAC CGAGATGAGCTTCCTGAACAGCGAAGTGTTGGCGGGGGACTTGATGTCCCCCTTCGACCAGTCGGGTTTG GGGGCTGAAGAAAGCCTAGGTCTCTTAGATGACTATCTGGAGGTGGCCAAGCACTTGAAACCTCATGGGT TCTCCAGCGACAAGGCGGGCTCCTCGGAATGGCCGGCTATGGATGATGGCTTGGCCAGTGCCTCAGACAC CGGCAAGGAGGATGCCTTTTCCGGGACAGATTGGATGTTGGAGAAAATGGATCTGAAAGAGTTTGACTTC GATGCTCTGTTTCGAATGGATGACCTGGAAACCATGCCAGATGAGCTCTTGACCACGTTGGATGACACAT GTGATCTTTTTGCCCCTCTAGTCCAAGAGACTAATAAGGAGCCCCCTCAGACAGTGAACCCAATTGGCCA TCTCCCAGAAAGTTTAATAAAAGTCGACCAGGTTGCCCCCTTTACATTCTTGCAGCCTTTCCCCTGTTCC CCAGGGGTTCTGTCTTCCACTCCAGAGCATTCCTTTAGTTTAGAGCTAGGCAGTGAAGTTGATATCTCTG AAGGAGACAGGAAGCCTGACTCTGCTGCTTACATTACTCTAATCCCTCCATGTGTAAAGGAGGAAGACAC TCCCTCTGACAATGACAGTGGCATCTGTATGAGCCCAGAGTCCTACCTGGGCTCTCCCCAGCATAGCCCC TCCACCTCCAGGGCCCCACCAGACAATCTGCCTTCTCCAGGTGGTTCCCGTGGGTCTCCTCGGCCCAAAC CTTATGACCCACCTGGAGTTAGTTTGACAGCTAAAGTGAAGACTGAGAAATTGGATAAGAAGCTGAAAAA GATGGAGCAAAACAAGACAGCAGCCACTAGGTACCGCCAGAAGAAGCGGGCTGAGCAGGAGGCCCTCACT GGCGAGTGTAAGGAGCTAGAAAAAAAGAATGAGGCTCTGAAAGAGAAGGCAGATTCTCTGGCCAAGGAGA TCCAGTATCTGAAAGACCTGATAGAAGAGGTCCGTAAGGCAAGGGGGAAGAAGAGAGTTCCGTAATAGGG TAGTCAGGTGCTTTGTGCTTGTACATAGTCTTGTGTTGCTGTGTTTGCTGTAATAAATTATTTTGTAGTG AAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_009716
• Insert Size: 1050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC085169, AAH85169
• RefSeq Size: 1717 bp
• RefSeq ORF: 1050 bp
• Locus ID: 11911
• UniProt ID: Q06507
• Cytogenetics: 15 37.85 cM
• Summary: Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to asymmetric CRE's as a heterodimer and to palindromic CRE's as a homodimer. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to ER stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.