Dct (NM_010024) Mouse Untagged Clone

SKU MC205245

Dct (untagged) - Mouse dopachrome tautomerase (Dct), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Dct
• Synonyms: DT; slaty; slt; TRP-2; TRP2; Tyrp-2; Tyrp2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC067064; GAACTGAGGCCATGCTTCGGATGAAATTAAATCAGTGTTAGTTGTTTGAGTCACATAAAATTGCATGTGC GTGTACACATGTGCACACGTGTAGGCTCTGTGATTTAGGTGGGAATTTTGAGAGGAGAGGAAAGGGCTAG AACTAAACCCAAAGAAAAGGAAAGAAGAGAAGAGGAAAGGAAAGAAAAAAGAAAAGGCAATTTGAGTGAG TAAAGGTTCCAGAACTCAGGAGTGGAAGACAAGGAGTAAAGTCAGACAGAAACCAGGTGGGACGCCGGGC CAGGCCTCCCAATTAAGAAGGCATGGGCCTTGTGGGATGGGGGCTTCTGCTGGGTTGTCTGGGCTGCGGA ATTCTGCTCAGAGCTCGGGCTCAGTTTCCCCGAGTCTGCATGACCTTGGATGGCGTGCTGAACAAGGAAT GCTGCCCGCCTCTGGGTCCCGAGGCAACCAACATCTGTGGATTTCTAGAGGGCAGGGGGCAGTGCGCAGA GGTGCAAACAGACACCAGACCCTGGAGTGGCCCTTATATCCTTCGAAACCAGGATGACCGTGAGCAATGG CCGAGAAAATTCTTCAACCGGACATGCAAATGCACAGGAAACTTTGCTGGTTATAATTGTGGAGGCTGCA AGTTCGGCTGGACCGGCCCCGACTGTAATCGGAAGAAGCCGGCCATCCTAAGACGGAATATCCATTCCCT GACTGCCCAGGAGAGGGAGCAGTTCTTGGGCGCCTTAGACCTGGCCAAGAAGAGTATCCATCCAGACTAC GTGATCACCACGCAACACTGGCTGGGGCTGCTCGGACCCAACGGGACCCAGCCCCAGATCGCCAACTGCA GCGTGTATGACTTTTTTGTGTGGCTCCATTATTATTCTGTTCGAGACACATTATTAGGTCCAGGACGCCC CTATAAGGCCATTGATTTCTCTCACCAAGGGCCTGCCTTTGTCACGTGGCACAGGTACCATCTGTTGTGG CTGGAAAGAGAACTCCAGAGACTCACTGGCAATGAGTCCTTTGCGTTGCCCTACTGGAACTTTGCAACCG GGAAGAACGAGTGTGACGTGTGCACAGACGAGCTGCTTGGAGCAGCAAGACAAGATGACCCAACGCTGAT TAGTCGGAACTCGAGATTCTCTACCTGGGAGATTGTGTGCGACAGCTTGGATGACTACAACCGCCGGGTC ACACTGTGTAATGGAACCTATGAAGGTTTGCTGAGAAGAAACAAAGTAGGCAGAAATAATGAGAAACTGC CAACCTTAAAAAATGTGCAAGATTGCCTGTCTCTCCAGAAGTTTGACAGCCCTCCCTTCTTCCAGAACTC TACCTTCAGCTTCAGGAATGCACTGGAAGGGTTTGATAAAGCAGACGGAACACTGGACTCTCAAGTCATG AACCTTCATAACTTGGCTCACTCCTTCCTGAATGGGACCAATGCCTTGCCACACTCAGCAGCCAACGACC CTGTGTTTGTGGTCCTCCACTCTTTTACAGACGCCATCTTTGATGAGTGGCTGAAGAGAAACAACCCTTC CACAGATGCCTGGCCTCAGGAACTGGCACCCATTGGTCACAACCGAATGTATAACATGGTCCCCTTCTTC CCACCGGTGACTAATGAGGAGCTCTTCCTAACCGCAGAGCAACTTGGCTACAATTACGCCGTTGATCTGT CAGAGGAAGAAGCTCCAGTTTGGTCCACAACTCTCTCAGTGGTCATTGGAATCCTGGGAGCTTTCGTCTT GCTCTTGGGGTTGCTGGCTTTTCTTCAATACAGAAGGCTTCGCAAAGGCTATGCGCCCTTAATGGAGACA GGTCTCAGCAGCAAGAGATACACGGAGGAAGCCTAGCATGCTCCTACCTGGCCTGACCTGGGTAGTAACT AATTACACCGTCGCTCATCTTGAGACAGGTGGAACTCTTCAGCGTGTGCTCTTTAGTAGTGATGATGATG ATGCCTTAGCAATGACAATTATCTCTAGTTGCTGCTTTGCTTATTGTACACAGACAAAATGCTTGGGTCA TTCACCACGGTCAAAGTAAGGTGTGGCTAGTTATATGTGACCTTTGATTAAAAGTCCTTATATTGAAAAA AAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_010024
• Insert Size: 1554 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC067064, AAH67064
• RefSeq Size: 2113 bp
• RefSeq ORF: 1554 bp
• Locus ID: 13190
• UniProt ID: P29812
• Cytogenetics: 14 61.6 cM
• Summary: Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA) (PubMed:1537333). Involved in regulating eumelanin and phaeomelanin levels.[UniProtKB/Swiss-Prot Function]