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Plcb3 (NM_008874) Mouse Untagged Clone

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SKU
MC204578
Plcb3 (untagged) - Mouse phospholipase C, beta 3 (Plcb3), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,075.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Plcb3
Synonyms mKIAA4098
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC035928
GACGCCGCGGGGCCAGAGCGGGCCGCGCGGGAGCGTGTGGAGAGAGCAGTGGCACCGTCGGTTCCCGTCG GGGCTCCGTAGGTCCCCGAGCCGCCCCTGGCTTAGCCATGGCGGGCGCGAGGCCCGGCGTCCACGCGCTG CAGCTGGAGCCGCCCACCGTGGTGGAGACCCTGCGGCGCGGGAGTAAGTTCATCAAATGGGACGAGGAGG CCTCCAGTCGGAACCTGGTGACCCTGCGTGTGGACCCTAATGGCTTCTTCTTGTACTGGACAGGACCCAA CATGGAGGTGGACACACTGGACATCAGCTCCATCAGGGACACAAGGACAGGTCGTTATGCCCGCCTGCCC AAGGACCCTAAGATTCGAGAAGTACTGGGCTTTGGAGGTCCTGACACCCGGCTGGAGGAGAAACTGATGA CAGTGGTGGCCGGGCCAGATCCAGTAAATACCACATTCTTGAACTTCATGGCCGTGCAAGATGACACAGT CAAGGTCTGGTCAGAGGAGTTGTTTAAACTGGCCATGAACATATTGGCTCAGAACGCCTCCCGGAACACC TTCCTGCGGAAAGCATACACGAAGCTGAAGCTGCAGGTGAACCAGGATGGACGGATTCCGGTCAAGAACA TTCTGAAGATGTTCTCGGCGGACAAGAAGCGGGTGGAGACGGCGCTGGAGTCCTGTGGCCTCAACTTCAA CCGAGTTGAGTCCATTCGCCCTGATGAGTTTCCCTTGGAAATTTTTGAGCGGTTCTTGAATAAACTGTGT CTGCGCCCAGATATTGACAAGATCCTGCTGGAGATAGGTGCCAAGGGCAAGCCGTACCTCACTCTGGAGC AGCTCATGGACTTTATCAACCAGAAGCAGAGAGACCCGAGACTCAACGAAGTGCTGTACCCGCCACTTCG GTCCTCACAGGCTCGGCTGCTCATTGAGAAGTACGAGACGAACAAACAGTTCCTGGAGCGCGACCAGATG TCTATGGAGGGCTTCAGCCGCTACCTGGGAGGGGAGGAGAATGGTATCCTGCCTCTGGAGGCCCTGGATC TGAGCATGGACATGACCCAGCCACTGAGCGCATATTTTATCAACTCCTCACACAACACCTATCTCACTGC GGGCCAGCTGGCTGGACCATCATCGGTGGAGATGTACCGCCAGGCACTGCTGTGGGGCTGCCGCTGTGTG GAGCTGGATGTATGGAAGGGACGGCCACCGGAGGAAGAGCCTTTCATCACTCATGGTTTCACCATGACCA CTGAGGTGCCATTGCGTGATGTGCTAGAGGCCATCGCTGAGGCAGCCTTCAAGACCTCGCCCTACCCTGT CATCCTCTCCTTTGAGAACCATGTCGACTCGGCAAAGCAGCAGGCCAAGATGGCTGAGTACTGCCGCTCT ATCTTTGGGGATGCGCTGCTCATTGACCCTCTCGACAAATACCCGCTATCTGCGGGCATCCCCCTGCCTA GTCCACAGGACCTGATGGGCCGTATCCTGGTGAAGAATAAGAAGCGACATCGGCCCAGCACAGGTGTCCC TGACAGCTCAGTGCGCAAGCGGCCTCTGGAACAGAGCAACTCGGCCTTGAGTGAGAGCTCGGCCGCCACA GAGCCCTCCTCACCTCAGCTTGGGTCCCCCAGCTCCGACAGCTGCCCTGGCCTAAGCAATGGGGAGGAGG TGGGACTCGAGAAGACCAGCCTGGAGCCTCAGAAGTCTCTAGGTGAGGAGAGCCTCAGCCGGGAACCTAA TGTGCCCATGCCTGACCGTGACCGAGAGGATGAGGAGGAAGATGAAGAAGAAGAGGAAACAACGGATCCA AAAAAGCCCACCACTGATGAGGGCACAGCCAGCAGTGAGGTCAATGCCACGGAGGAGATGTCAACGCTCG TCAACTACGTTGAGCCCGTCAAGTTCAAGTCCTTCGAGGCTGCTCGAAAAAGGAACAAGTGCTTCGAGAT GTCATCCTTCGTGGAGACCAAGGCAATGGAGCAACTGACCAAGAGTCCTATGGAGTTTGTGGAATACAAC AAACAGCAGCTCAGCCGCATCTACCCTAAGGGCACACGAGTGGACTCCTCCAACTACATGCCTCAGCTCT TCTGGAACGTGGGTTGCCAGCTTGTTGCCCTCAACTTCCAGACCTTGGATTTGCCAATGCAGCTCAACGC AGGCGTGTTTGAGTACAATGGGCGCAGCGGGTACTTGCTCAAGCCCGAGTTTATGCGGCGGCCGGACAAG TCTTTTGATCCCTTCACTGAGGTCATCGTAGATGGCATAGTGGCCAACGCCTTGCGGGTCAAGGTGATTT CGGGGCAGTTCCTGTCTGACAAGAAGGTGGGCATCTACGTCGAGGTGGACATGTTTGGCCTCCCAGTTGA CACAAGACGCAAATATCGTACCCGGACATCCCAGGGGAACTCATTCAACCCTGTGTGGGACGAGGAACCC TTTGACTTCCCCAAGGTTGTGCTGCCCACGCTGGCCTCACTTCGCATCGCAGCCTTTGAGGAGGGTGGCA AATTTGTTGGGCACCGTATCCTGCCTGTCTCTGCTATCCGCTCAGGATACCACTATGTCTGCCTGCGAAA CGAGGCCAACCAACCTTTGTGCCTGCCTGCCCTGCTTATCTACACTGAAGCTTCTGACTACATCCCAGAT GACCACCAGGACTATGCGGAGGCCTTGATTAACCCCATCAAGCACGTAAGCCTAATGGACCAGCGGGCCA AGCAACTAGCCGCTCTCATTGGGGAGAGTGAGGCTCAGGCCAGCACAGAGACATATCAGGAGACCCCGTG TCAACAGCCAGGGTCACAGCTCCCCTCCAACCCCACACCTAACCCACTGGATGCCTCACCTCGCTGGCCC CCCGGTCCTACCACTTCCTCCACTAGCTCCTCCCTCAGTAGCCCAGGGCAGCGAGATGATTTGATTGCCA GCATCCTCTCTGAGGTGACCCCTACACCTCTAGAGGAGCTCAGAAGCCACAAGGCTATGGTGAAGCTACG GAGCCGGCAGGATCGAGACCTGCGGGAGTTGCATAAGAAGCACCAGCGCAAGGCTGTGGCCCTCACCCGC CGCCTTCTGGATGGCCTGGCCCAGGCCAGGGCTGAGGGGAAGTGCCGGCCCTCCCCCAGTGCCCTGGGCA AGGCCACCAACTCGGAGGACGTGAAGGAGGAAGAGGAGGCCAAACAGTATCGAGAGTTCCAGAACCGACA AGTACAGAGCCTGTTGGAGCTGAGGGAAGCCCAGGCAGATGTGGAGACCAAGCGGAAGCTGGAGCATCTA CGACAGGCTCATCAGCGGCTCAAGGAGGTTGTCCTGGATACACACACAACACAGTTCAAGAGGCTGAAGG AGCTGAATGAAAGGGAGAAGAAGGAACTCCAGAAGATCCTGGACAGGAAGCGCAACAACAGCATCTCAGA GGCCAAGACAAGGGAGAAACACAAGAAGGAGGTGGAACTGACAGAGATTAATCGGCGGCACATCACTGAG TCGGTAAACTCCATCAGACGGCTGGAAGAGGCCCAGAAGCAGCGCCATGAACGCCTGGTGGCAGGGCAAC AGCAAGTCCTCCAGCAGCTAGAGGAAGAGGAACCCAAGCTGCTGGCCCAGCTGACCCAGGAGTGTCAGGA ACAGCGAGAGAGGTTGCCCCAGGAGATCCGTCGGTGCCTGCTGGGCGAGACAGCAGAGGGACTGGGGGAC GGCCCCCTGGTGGCCTGTGCCAGCAATGGTCATGCACCTGGGAGTGGTGGGCACCTGTCCAGCGCTGACT CGGAGAGCCAGGAGGAGAACACCCAGCTTTGAACTGGCTGAGCAAGAGGTGGCCACAAGTCTGGGTGGGC ACTGGGCTATGGGTAGAAGACACTAATACTTTGGGTTTTTTTTAACCTTTTATCTTTAGAAATTTTATTT TTTTAAAATCTGAGGCAAATGCCTCACACTAACTCTAACTCCCTGCATCTTCCTGGGACCTCTTGGTCCT CAGCCTCCCCTTCTAACTTCAGGCCTAGGGAAAGGGCTAGGGCTTGTACCCCATAACATTCACCTAAGAT AGGTACTCCCCGATTCCCAAGGAGCAGCTAGTGGAACCCTGGAGTTCTGGGTAGTAGAAAGCAACCCTTT ATATTTGGGGAGTTTTGTTTGTTTGTTTGTTTGTTTTTTACATGAATAAAAGTGGACTTCAGGGAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_008874
Insert Size 3705 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC035928, AAH35928
RefSeq Size 4227 bp
RefSeq ORF 3705 bp
Locus ID 18797
UniProt ID P51432
Cytogenetics 19 5.1 cM
Summary The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) uses two alternate splice sites that result in an alternate codon in the 5' coding region, compared to variant 1. The encoded isoform (b) is the same length as isoform a.
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Documents
Resources
"NM_008874" in other vectors (4)
SKU Description Size Price
MG211834 Plcb3 (tGFP-tagged) - Mouse phospholipase C, beta 3 (Plcb3) 10 ug
$1,274.00
MR211834 Plcb3 (Myc-DDK-tagged) - Mouse phospholipase C, beta 3 (Plcb3) 10 ug
$589.00 $1,074.00
MR211834L3 Lenti ORF clone of Plcb3 (Myc-DDK-tagged) - Mouse phospholipase C, beta 3 (Plcb3) 10 ug
$1,374.00
MR211834L4 Lenti ORF clone of Plcb3 (mGFP-tagged) - Mouse phospholipase C, beta 3 (Plcb3) 10 ug
$1,374.00

file-alt Citations

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