Grk2 (NM_130863) Mouse Untagged Clone

SKU MC204540

Adrbk1 (untagged) - Mouse adrenergic receptor kinase, beta 1 (Adrbk1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Grk2
• Synonyms: Adrbk-1; Bark-1; beta ARK; betaARK1; GRK2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC053922; CAAGGCCACGCCGGCCGCGCGCGCCAGCAAGAAGATCCTGCTGCCAGAGCCCAGGGGACCTCCCATCCTA CAGCAGATGGACCATGCTGCCACCTCTCTACAAGACAACTGATGTCATCGCACCTGCATCCGCAGCGTCA TGCAGAAGTATCTGGAGGACCGAGGAGAAGTGACCTTTGAAAAGATCTTTTCGCAGAAGTTAGGGTACCT GCTTTTCCGGGACTTCTGCCTGAACCATCTGGAAGAGGCCAAGCCCTTGGTGGAGTTCTACGAAGAGATC AAGAAATATGAGAAGCTGGAGACAGAGGAGGAACGTGTGGTCCGCAGCCGAGAGATCTTTGACTCCTATA TTATGAAAGAACTGCTGGCCTGCTCACATCCCTTTTCAAAGAATGCTACGGAGCATGTCCAGGGCCACCT GGTGAAGAAGCAGGTGCCTCCAGATCTCTTCCAGCCATACATTGAGGAGATTTGTCAGAACCTCCGAGGG GACGTGTTCCAGAAGTTCATAGAGAGTGACAAGTTCACACGGTTCTGCCAGTGGAAGAATGTTGAGCTCA ACATCCACCTGACCATGAATGACTTCAGTGTGCATCGAATCATCGGGCGTGGGGGCTTCGGTGAGGTCTA TGGGTGCCGGAAAGCAGACACAGGCAAGATGTACGCCATGAAGTGTCTGGACAAGAAACGCATCAAGATG AAGCAGGGAGAGACCCTGGCTCTGAACGAGCGGATCATGCTTTCCCTCGTCAGCACCGGGGACTGCCCCT TCATCGTGTGCATGTCATATGCATTCCACACACCAGACAAGCTCAGCTTCATCCTGGACCTCATGAACGG TGGGGACCTGCACTACCACCTGTCTCAGCATGGAGTCTTCTCTGAAGCCGACATGCGCTTCTACGCCGCT GAGATCATCCTGGGCCTTGAACACATGCACAATCGCTTTGTAGTCTACAGGGACCTGAAGCCAGCCAACA TTCTCCTGGATGAACATGGCCATGTGAGAATCTCGGACCTGGGCCTAGCCTGTGACTTCTCCAAGAAGAG GCCTCATGCCAGTGTGGGCACACACGGGTACATGGCCCCCGAAGTCCTACAGAAGGGCGTGGCCTATGAC AGCAGTGCAGACTGGTTCTCCCTGGGCTGCATGCTCTTCAAGTTGTTGCGGGGACACAGCCCCTTCCGGC AGCACAAGACCAAAGACAAGCATGAGATTGACCGCATGACGTTGACGATGGCTGTAGAGCTGCCTGACTC CTTCTCCCCTGAACTCCGATCCCTGCTGGAAGGTTTGTTGCAGAGGGACGTCAATCGGAGACTAGGCTGT CTGGGCCGTGGGGCTCAGGAGGTAAAAGAAAGTCCTTTCTTTCGTTCCCTGGACTGGCAGATGGTCTTCT TACAGAAGTACCCTCCTCCACTGATCCCCCCACGTGGGGAGGTGAATGCAGCTGACGCTTTCGACATTGG CTCCTTTGATGAGGAGGACACAAAAGGAATCAAGTTACTGGACAGTGACCAGGAACTGTACCGCAACTTC CCTCTCACCATCTCTGAGCGGTGGCAACAGGAGGTGGCAGAGACTGTCTTTGATACCATCAATGCTGAAA CAGACCGGCTGGAGGCACGAAAGAAGGCCAAAAACAAACAGCTGGGCCACGAGGAGGACTATGCCCTGGG TAAGGACTGCATCGTGCATGGCTACATGTCCAAGATGGGCAACCCCTTCCTGACCCAGTGGCAGCGGCGA TACTTCTACTTGTTCCCCAACCGGCTCGAGTGGAGGGGTGAAGGCGAGGCTCCGCAGAGCCTCCTGACCA TGGAGGAGATCCAGTCAGTGGAAGAGACACAGATCAAGGAACGCAAGTGTCTCCTGCTTAAGATCCGAGG TGGCAAGCAGTTTGTCCTGCAGTGTGATAGTGATCCAGAGCTGGTGCAATGGAAAAAGGAGCTGCGTGAT GCCTACCGCGAGGCCCAGCAGCTGGTACAGCGAGTACCCAAGATGAAGAACAAGCCACGCTCGCCTGTGG TGGAGCTGAGCAAGGTGCCGCTGATCCAGCGTGGCAGTGCCAACGGCCTCTGATCCACCCATGTGCTGCC CGCCTGCCCACCCGCCTTTTATAAACCTCTAATTTATTTTGTTGAATTTTTATTATTTGTTTTCCCGCAA AGCGGAAAATGTTTTATTTTGTAATTATTGTGATCTCCTGTGGCCCCAGCCTGGCCCAGCCCCCAGGGAG GGGCCCGCTTGCCTCAGCTCCTGCTGCCACCAACCCAGCCACTGTCTAGCACCCCTCCCCTGTCCCCACT CCCACCCCCTAGGGTCCTTGCTCCCAGTATCTTCCTACAGGGAAGAAGAGCAACCCTCAGCAGCCCTCTT GCCCCTCCATGGGGTGAAGCCACACTCAGTGGGTGTGTCACTTCAGGCTCTGTGGGCTGTGCTCAGTCTG TGGCCCAGCACCCCTGCCCTAGAAAGGGGCAACACAGGGTACTACTTGAACTTTCCTATCACAGCTCCCA TGCCACAGAGGGACAGACCCCTGCTGTCCTGCCCTCTTGCCACTGGTGAGAGAAGGCCCTTGTCTCCTCA GACTAGGAGGTCTCCCACAGTGACAGCTCCTGGGTCCTTGTTCAGGAAAAGCCTGTGTGTTACCACTGCC TCATCAGGAGGCTGCCATTGGCAGTGGCTGCTGCTGGCCTCCCTGCAAGTCCCCTCTTCTTCCTGTTGCG TGCCTGGACTCAGGAGCATAGAAAAGACAATTGTGGATTGGGCCACACTGGCCTAGCCTGAACCTAGATC CCTAACCCTGGGCTGGGTGAGGCTCACCTGCCCAGGACCACAGTGGGACAGCCTGGGCAGGTGGGTGGCA CAGCATAAGAACCCAGCTGGGGCCAGCCCTCCCTGTCCTGGCTGGTCAGTGTGCCCCCACCCACACTGTC CAGCCCCTTAGCCCATGTCATGTCAGTGGCCACTGCCCACAGGTGCCACCTTGCCCACCGCATGACCCCT TGTGCCAGTCGCGCAGCTGTGTGTGGTGTCGCGCCCTCTCCCCTTGGGGGCTGGGTGGGCACACCCTCCC CTCTTGTCTACTCACTCCTAGGGCGTTTCTTTGCCGATTTTTGAATGTGATTTTAAAGAGTGGAAAATGA GACTATGCGTTTTTATAAAAAATGGTGCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_130863
• Insert Size: 1944 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC053922, AAH53922
• RefSeq Size: 3332 bp
• RefSeq ORF: 1944 bp
• Locus ID: 110355
• Cytogenetics: 19 3.98 cM
• Summary: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by faciltating the trafficking of SMO into the cilium and the stimulation of SMO activity.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) contains an alternate exon in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.