Mios (NM_145374) Mouse Untagged Clone

SKU MC204519

Mios (untagged) - Mouse missing oocyte, meiosis regulator, homolog (Drosophila) (Mios), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Mios
• Synonyms: C81488
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC020002; AGGCGGGGCTCACGGCGAGGAGGTCTGCTTCGGAGGCGGCAGGGTGGTTTGGTCGGGGGCCCAGCGGGTG GCCCGGGTCACCGCCCGCGCTTCGGCGGCCATCTTGCCCGCGCCCGTCTCCCGCGGCGGGAGGGGCGGCG TACAGGCCGGAAACGTCTGTGTGGCGACAGGCCACCTCCTCAGGGACATAGCAAGAATCTCAAGACATGT TTCAGAAGCTCCCTCTATTTGTGAGAATCTAGTGAATGGACCTGACTGGACTTTTTAAGCCCATCAATAA ACATGAGCGGTACCAAGCCCGATATTTTGTGGGCACCACACCAAGTTGATAGATTTGTTGTATGTGACTC AGAATTGAGCCTTTATCATGTGGAATCGGCTGTGAATTCAGAACTCAAAGCTGGATCGTTACGTTTATCT GAAGACTCTGCAGCGACATTGCTGTCAATAAACTCAGATACACCCTATATGAAATGTGTTGCGTGGTATC TCAATTATGATCCTGAATGTCTCCTAGCAGTTGGACAAGCAAATGGTCGAGTTGTACTTACAAGTCTTGG TCAAGATCATAACTCTAAGTTCAAAGATTTGATAGGAAAAGAATTTGTTCCAAAGCATGCTCGCCAATGC AATACCCTTGCATGGAATCCACTGGATAGTAACTGGCTTGCTGCTGGTCTCGACAAACACAGAGCTGACT TTTCAGTTCTAATATGGGATATCTGCAGCAAATATACTCCTGATATTGTTCCCATGGAAAAAGTGAGACT TTCAGCAGGTGAAGCTGAAACAACATTACTAGTGACAAAACCGCTGTATGAGTTAGGGCAGAATGACGCT TCTCTATCTCTGTGTTGGCTTCCACGAGACCAGAAACTTCTTCTTGCTGGCATGCATCGTAATCTAGCAA TATTTGATCTCCGGAACACAAGCCAGAAGATGTTTGTAAATACAAAAGCTGTTCAAGGAGTGACAGTAGA CCCTTACTTCCACGATCGTGTTGCTTCCTTCTATGAAGGTCAGGTTGCAATATGGGATCTTAGAAAATTT GAGAAGCCAGTTTTGACTTTGACTGAACAACCAAAGCCCTTGACAAAGGTCGCATGGTGTCCAACCAGGA CAGGCCTGCTTGCTACTTTAACAAGGGATAGTAATATTATTAGGCTGTATGATATGCAGCATACACCCAC TCCCATCGGGGATGAAACCGAACCCACAATAATTGAAAGAAGTGTGCAGCCTTGTGACAATTACATTGCT TCCTTTGCATGGCATCCAACGAGTCAAAATCGAATGATAGTTTCGACTCCCAACCGAACAATGTCTGACT TCACTGTATTTGAAAGGATATCTCTTGCCTGGAGCCCAATTACATCTTTAATGTGGGCTTGTGGTCGCCA TTTGTATGAGTGTGCAGAAGAAGAAAGTGATAACTCCTTAGAAAAAGATATAGCAACAAAAATGCGCCTT CGGGCTTTATCAAGGTATGGACTTGATACAGAACAGGTTTGGAGAAACCACATTTTAGCTGGCAATGAAG ACCCACAGCTCAAGTCACTCTGGTATACTCTGCACTTTATGAAGCAATATACAGAAGATAATGATCAAAA ATCTCCAGGCAACAAAGGATCATTGGTTTATGCAGGAATTAAATCAATAGTAAAATCATCTTTGGGAATG GTAGAAAGCAGCAGACATAATTGGAGTGGCTTGGATAAACAAACTGATATTCAGAACTTAAATGAAGAAA GGATTTTAGCATTACAGCTTTGTGGGTGGATAAAGAAAGGAACGGATGTAGATGTAGGGCCATTTTTGAA CTCCCTTGTGCAAGAAGGAGAATGGGAAAGAGCTGCTGCTGTGGCTTTGTTCAACTTGGATATTCGGCGA GCAATCCAAATCCTGAATGAAGGGGCGTCTTCAGAAAAAGGAGATCTGAATCTCAATGTGGTGGCAATGG CTTTATCGGGGTATACGGACGAGAAGAACTCGCTGTGGCGGGAGATGTGCAGCACCCTGCGCCTGCAGCT CAACAACCCCTATCTGTGTGTCATGTTTGCGTTCCTGACGAGTGAAGCGGGGGCATATGATGGAGTGTTG TATGAAAACAAAGTTGCTGTACGTGACAGAGTGGCATTCGCTTGTAAATTCCTTGGTGATGCTCAGTTAA ATAAATACATTGAAAAGCTGACCAATGAAATGAAAGAGGCTGGAAATTTGGAGGGGATCTTGCTTACAGG CCTCACTAAAGATGGAGTGGACCTGATGGAGAGCTATGTGGATAGAACTGGAGATGTTCAAACAGCAAGT TACTGCATGTTACAGGGTTCACCTTTAGATGTTCTGAAAGATGAAAGGGTTCAATATTGGATTGAGAATT ACAGAAATTTATTAGATGCCTGGAGGTTTTGGCACAAAAGGGCAGAATTTGATATTCATAGGAGTAAGCT GGATCCCAGTTCCAAGCCTTTAGCACAGGTGTTTGTGAGTTGTAATTTTTGTGGCAAATCAATCTCCTAC AGCTGTTCCTCTGTGCCTCATCAGGGCAGAGGGTTTAGTCAGTATGGTGTGAGTGGCTCACCAACAAAAT CCAAAGTCACAAGCTGCCCTGGCTGTCGGAAACCCCTTCCTCGATGTGCACTTTGCCTCATTAATATGGG AACACCTGTTTCTAGCTGTCCTGGTGGATCCAAATCAGATGAAAAGGTGGACTTGAGCAAGGACAAAAAG TTAGCTCAGTTTAACAACTGGTTTACCTGGTGTCACAACTGTCGGCATGGTGGGCATGCCGGACATATGC TGAGTTGGTTCAGGGACCATGCAGAGTGTCCTGTATCTGCATGCACGTGTAAATGTATGCAGTTGGATAC AACAGGAAATCTGGTGCCAGCAGAGACTGTCCAGCCATGAAATGTTCCACCTAAAGAAAAATCTTCACAT GTGGAGTTTTTAAAGGATGTCCTTCATAGTTCAGGAAATAAAGTCACTCATGGCTTTGCTGTCGTGGAAA AATAAAGCATTCTGTCAGCTCAGCAATGTGATGTTTGAGTGACTTTGACGTACTCCTCAGAGGCCAACGC TGCTGATAGGAACATTAGAACATGAATATTGGTGCTGTTTAGTAGGTTAAAGTTCATTTGAAAGAACTGT CTAGGCTTTTGTCAAAATTATGTTTATAGATGGTTTTAGAATAAAAACAAGCCTTGTGCCTGCATCTTCA ATCTGAAATTGATATCTTTTGATAAAATCTGTACATATCTAAAACCAAATGCCAATTCATTGTTTCATAT ATCAATTTAAGCTTATTCATGCTTAAATATGCTTTCAGAGAAAATGTCAATTTTACATTGGTAATTAGCT CCTGGCCCATGCTGGAGAATACATGCATGTGTGCATATGTGCACACACACGTTTAAGGAACGTTCTCCCT CATACATTTATATTTTAATATAAAAGATTTGACATAGGTCATTCTTGTCTTCCTCAAAACTCCAAGTTAA AAGCAGGCTGGTGAGCTGGTTCAGCATGCGAGGAGGCTTGCAGCCAATCCTGATGACCTAAGTGCGGTCC CATGTGTTGGAAGCAGTGATTCAACTCATCTCCAGAACCTTGCTGCAGCATGGGGCGAGCACGTACACAT GTGCGCGCTCGTGAGCACTCGAGCACACGCAGACAATAAATAAAAAAAATTTAAAACGATAAAAAAAAAA AAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_145374
• Insert Size: 2628 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC020002, AAH20002
• RefSeq Size: 3715 bp
• RefSeq ORF: 2628 bp
• Locus ID: 252875
• UniProt ID: Q8VE19
• Cytogenetics: 6 A1
• Summary: As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex. It is negatively regulated by the upstream amino acid sensors SESN2 and CASTOR1.[UniProtKB/Swiss-Prot Function]