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Ube3b (NM_054093) Mouse Untagged Clone

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SKU
MC204494
Ube3b (untagged) - Mouse ubiquitin protein ligase E3B (Ube3b), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $932.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ube3b
Synonyms AI449831; AU020130
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC023956
CCACGCGTCCGTGGCGGCCGGGCTTCGGCCGCTGCCCCGGGGCTGGCGGACCTCGGGGGGATCGGGCTAA GGCGGCGGTCGCTGCGGCCCGGACCCCGAGTGCGGGGCCTCGAACGGATAAAGATCTCCGGACTGCGGGC CAGAAAACCCAACTCCTCGGAAGCCAGAGGCTGCAGGAGGTCACTGATGGTCACCTAGCATCTCCGCTGC ACAGGCAGGTCCTCAGGCTTCGTCGTGTCAGTTTGCAAACATGTTCACTGTATCTCAGACCTCCAGAGCA TGGTTCATCGACAGAGCCCGGCAGGCACGAGAAGAGAGGCTGGTGCAGAAGGAGCGGGAGCGCTCAGCGG TGACGATCCAGGCCCTAGTCCGCAGTTTCCTCTGCCGGAGGCGACTGCACAGAGACATCAGGAAAGAGAT TGATGAGTTTTTTAGTGCTGATGAGTCTGGGTCCAGTAAAAGAAGTGCACTTTGTATTTTTAAGATTGCC AGGAGACTGCTGTTTATATGCAAAACTACCGAGGACAGCGAGAGACTGGAGAAGTTGTGTCGAAGCATCC TGAACAGCATGGATGCCGAGAATGAGCCAAAGGTGTGGTACGTGTCCCTGGCTCTCTCCAAGGACCTCAC GCTGCTGTGGATTAAGCAGATCAAGAGCATCCTGTGGCACTGCTGTGAGCTTCTGGGGCAGCTCAAGCCA GAGATCTTACAGGACTCCAGGCTCATCACGCTGTACCTCACCATGCTTGTGACCTTCACAGACACTTCAA CATGGAAGATTCTCCGGGGAAAAGGTGAGAGCCTTCGGCCAGCCCTGAACCACATTTGCGCAAATATAAT GGGCCATCTCAACCAGCGTGGACTTTACTCTGTATTGCAGGTACTGCTGACCCGAGGCCTGGCCAGGCCC CGGCCCTGTCTGTCCAAAGGCATGTTAACAGCAGCCTTCTCTCTGGCCCTGCGCCCTGTGGTTGCTGCGC AGTTCTCAGACAATCTTATGCGACCATTCATCATCCACGTCATGTCGGTACCTGCTCTCGTGGCTCATCT CAGCACCGTGGCCCCTGAGCGCCTCGGCGTTTTAGAGTCCCATGACATGCTTCGTAAATTCATCGTCTTC CTGAGAGATAGAGACCGATGCCGCGATGCTTGTGAGAGTTTAGAAGGCTGCCATACCCTGTGTCTCATGG GCAATCTCCTGCACTTGGGCTCCCTCAGCCTCAGGCTGTTAGAGGAAGAGATGGATGGGTTTGTGAGCGC GCTCACGCAGATGCTGTGCTACTGTCAGAAGTACGTGGCTCAGAAGAAGTCCAACCTGACCCACTGGCAC CCTGTGCTCGGCTGGTTTTCCCAACCTGTGGATTACGGCCTCAATGATTCCATGTACCTGATCACCAAGC AGCTGCAGTTCCTGTGGGCGGTGCCTCTCATCCGCATTCTCTTCAGTGACATCCTGAGCAGGAAGCTGCT GGAGCATGCAGAGCCGGCCCCGGTGCAACCGCAGCCGTCCTCACCGCAGACGGTGCTCCCCGTGAAGAGC CTCCTCAAGCGTGCGTTTCAGAAGTCGGCTTCGGTTCGGAACATCCTCAGGCCTGTTGGGGGCCGCCGTG TCGACTCCGCTGAGGTGCGGAAGGTCTGCAACATTTGCGTGCTCTACCAGACCTCGCTGACGACCCTCAC CCAGATCCGCCTGCAGATCCTCACAGGTCTTACGTACCTGGATGACCTGCTGCCCAAGCTCTGGGCGTTT ATCTGTGAGCTGGGGCCCCACGGCGGGCTCAAGCTCTTCCTGGAGTGCCTGAACAATGACACTGGAGAGT CCAAGCAGCTCCTGGCCATGCTGATGCTCTTCTGTGACTGTTCCCGGCACCTCATCACCATCCTCGATGA CATTGAAGTTTATGAAGAACAGATCTCCTTCAAGCTGGAAGAGCTGGTCACCATCTCCTCGTTCCTCAAT TCCTTCGTGTTTAAGATGATCTGGGACGGAATTGTAGAGAATGCCAAGGGTGAGACCTTGGAGCTGTTCC AGTCCGTCCATGGCTGGCTGATGGTGCTGTATGAGCGAGACTGCCGCCGGCGCTTTGCTCCGGAGGACCA CTGGCTACGGAGGGACCTCAAGCCTGGTGTGCTCTTCCAGGAGCTCGACAAGGACCGAAGGAGGGCTCAG CTGGTCCTGCAGCACATCCCCCACGTCGTGCCTCACAAGAACAGAGTTCTTCTGTTCCGGAACATGGTTA TCAAGGAGAAAGAGAAGCTGGGGCTTGTGGAGACCAGCTCTGCCTCTCCTCACGTCACCCACATCACCAT CCGAAGGTCCCGGATGTTGGAGGACGGCTACGAACAGCTCCGGCAGCTTTCCCAGCATGCCATGAAGGGT GTGATCCGAGTGAAGTTTGTCAACGACCTCGGGGTGGACGAGGCTGGGATTGACCAGGATGGTGTCTTCA AGGAGTTTCTGGAAGAGATCATCAAGAGGGTGTTTGACCCCGCACTCAACCTGTTCAAGACAACCAGCGG GGATGAGCGGCTGTACCCATCACCCACGTCCTACATTCACGAGAATTACCTGCAGCTCTTCGAGTTTGTG GGGAAGATGCTGGGAAAGGCAGTGTATGAGGGGATCGTGGTAGATGTGCCCTTTGCCTCCTTCTTCCTGA GCCAGATGCTCGGGCACCACCACAGCGTCTTCTACAGCTCCGTGGATGAGCTTCCCTCCCTGGACTCGGA GTTTTATAAAAACCTCACCTCCATCAAGCGCTACGATGGAGACATCGCAGACCTCGGGCTGACGCTGTCT TACGATGAAGACGTCATGGGTCAGCTCGTCTGCCACGAACTGGTTCCCGGAGGGAAGACCATCCCGGTTA CAGATGAGAACAAAATTAGCTACATCCATCTGATGGCGCATTTCCGAATGCACACTCAAATTAAAAACCA GACAGCTGCTCTTATCAGTGGCTTCCGTTCCATCATCAAGCCCGAGTGGATCCGAATGTTCTCTACCCCT GAGCTTCAGCGCCTCATCTCCGGGGACAACGCTGAGATAGATCTGGAAGACTTAAAGAAGCACACAGTGT ACTACGGTGGCTTTCATGGGAGCCACAGGGTCATCATCTGGCTCTGGGATATCCTGGCTTCCGACTTCAC GCCTGAAGAAAGAGCCATGTTCCTGAAGTTTGTGACCAGCTGCTCCCGGCCCCCGCTCCTAGGATTCGCC TACCTCAAGCCTCCGTTCTCCATCCGCTGTGTGGAAGTATCAGATGACCAGGACACTGGGGATACCCTGG GCAGCGTCCTCAGGGGCTTCTTCACCATCCGCAAGAGGGAGCCAGGGGGGCGTCTGCCCACATCTTCCAC TTGCTTCAACCTGCTTAAGCTGCCCAACTACAGCAAGAAGAGCGTACTGCGAGAGAAACTACGCTATGCC ATCAGCATGAACACGGGCTTTGAGCTCTCCTAACACAGCCTGGTCTCGCTACCACAGAGCCCCCAGGAGT GTGGCCAGGGTACCCACAGCCCTCAGACCCTCTCAATAGCCATGAGACACCCAAGGTTGGGGATGCTACA CAGTGCCCTCCATGCACTGGGGACAGAGGGCTGATGAAAATAACCCCCCAGTTCTGCCCTGTGGGGCCTG AGACCTGGGCCTAGTGATTACAGAGCAACTCCTGGAGATTCTGTGAGGCTTTCTTCTCTTGCCCTCTGAG GAGGACACAAGGCCTGTGCCTGTGAGCTCCTCCTTCCGTTCTGAAAACTCAGGTACGGCCTCGCCAAGCC TCTATGCACAGCAAAGTCCCTGCCTCTACCCTGTCCACAGTGGCCTCTTCCCAGGCAACCAAGCCCAGCT CCTGCCAGGGAGGCCACTGAGCACAGTCAGGGTCTCTCCTATAGGGATCCAGGTGGTCCACACCACAGCC CTCCTTCCCAAGTCCTCAGCAGGCCACGCTGTCAGGGAACCCTTGCTGTCAGCCCCTGGCCTGTGCATCA GTGTCCTAGCTTGGGAAAGAAACCCATCGTTCCCCTGGAGAACAGCCCATTTCTGGAGTACCCAGAGCCT GCTCGCTCTGGGCATCTGCCCCCTATTCACCTTACGGAGAACCACATGAGACCCTACCTACAGTGCCCAG CCGCCTGGGCAGTACTTTATTCCAGGGACTTCAGAGTCCGTCCTTTTGCACTGACAGGCCCTGGGAGCTG AGGATACGTCCCACACATCTCATCTCCACACCGCCTCTGTTGGCGCTTGCTCTTTTGTCTGGGACCTCAT TCTCTTTTCCCACACACTGCGCTCCTGGGCCCTCCCTTTCCCCATTTCCCTTAAGAACTTTCTCCAGATT CAGGATGTTGAGGCAGAGGCCCAGGGCTTAGTAACCTGCTAGTAGCCACTTGTCTCCTGCCTCATCAGGT TCTTGTCTCCCTCCCACCCTGATGGAGGAGTCCTGGCCCCTCCTCCACTCCGTGGAGCCAGCTTAGGGCT ACAGCGCTCAAAGCAAGAAAACGCCTTCCCCAAGGAGCCTGCACAGTGGGAAGTGGCCGTGCCCTGTTCT CACTTTAGCAAAGATCTTGGGCTGGAGCATGGGGTAGGCCCAGGAAAGCCCCTGGGGTTCTGGAAGGTGG CTCCAGCTTCACGTCTGCCAACCATGTGTGTTCCCAGCTTGCCCGCAGAGCCCTGTGCCCCGTGCACAGC CTGCACAGGTAACACAGAGCCTGAGAGTTACAGCGGGCACACGGGAGAGTTAGTTGTAAGCACCGATATC AACTTGACCCCTTTGTCTTTGACCTAGGGCTGTCTGTCGGTCACCTAGAGTCTGCTTCCCGGCAGTTATG TCATAGCTGCTGTTTGATGACATCTCAGCAGCTCCTGGTGGCGCCAGAAGGAAGCAGGGGTTTTTGTTTT TGTAGGTAACTCGTGGACTAAGACTTGTCAGGGCTTTATTAAATTTGTACTTGAGCACAAAAAAAAAAAA AAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_054093
Insert Size 3213 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC023956, AAH23956
RefSeq Size 4982 bp
RefSeq ORF 3213 bp
Locus ID 117146
UniProt ID Q9ES34
Cytogenetics 5 F
Summary E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Resources
"NM_054093" in other vectors (4)
SKU Description Size Price
MG211580 Ube3b (tGFP-tagged) - Mouse ubiquitin protein ligase E3B (Ube3b) 10 ug
$1,132.00
MR211580 Ube3b (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3B (Ube3b) 10 ug
$589.00 $932.00
MR211580L3 Lenti ORF clone of Ube3b (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3B (Ube3b) 10 ug
$1,232.00
MR211580L4 Lenti ORF clone of Ube3b (mGFP-tagged) - Mouse ubiquitin protein ligase E3B (Ube3b) 10 ug
$1,232.00

file-alt Citations

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