Mepce (NM_144913) Mouse Untagged Clone

SKU MC204098

Mepce (untagged) - Mouse methylphosphate capping enzyme (Mepce), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Mepce
• Synonyms: Bcdin3; D5Wsu46e
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026876; CACTCGGGGAAGGAGGGAGTGAGGTGGTCTTAGGAAGGGTTACAGGGGGCTTCCCCTTGCTCTCTTCTCA GTACCCTCGCTTGAGAAGGGGAAATTAAACAGGGAGAGGAGATGATCGAGATGGCGGCGGAGAAGGAGCC CTTTCTGGTGCCGGCCCCGCCGCCGCCGCTCAAAGATGAGTCGGGCGGAGGGGGCGGCCCCGAGGTGCAA TCGCACCAAGAGGCTGCCTCCGGGGAGCTCCGTGATGGGACAGAACACGGGCCGGGCCCTCGCGCGCACT CGGCCGGGGCCGCAGCCAGCGGAGGGGGCGGCCCCCAGGCACAGGCGCACGGGGAGCCCCACGGGCGAGC CGCCGCTCCTGCGGACGTGGGGGAGGAGCGCCGGGGAGGGGGCGGGACGGACCTGGGTCCCCCAGCCCCT CCTCGACCTCGCAATGGCTACCAGCCCCACCGACCCCCTGGGGGTGGTGGGGGCAAAAGAAGAAATAGCT GTAACGTGGGGGGAGGGAGCGGTGGAAGCTTCAAACATCCGGCCTTCAAGAGGCGCCGGCGAGTGAATTC GGACTGCGACTCTGTGTTACCATCCAATTTCCTTCTGGGGGGTAACATATTTGATCCACTGAACCTGAAT AGCCTCCTGGATGAGGAAGTGAGTCGCGCACTCAATGCGGAGACCCCAAAGTCATCCCCACTTCCGGCCA AGGGGCGAGACCCTGTGGAGATCCTCATCCCCAAAGATATCACTGACCCGCTCAGTCTCAACACTTGCAC TGACGAGGCCCATGTAGTGCTTGCCTCACCACTCAAGATTGGTCGCAAGCGCCATAGACACCGGGGACCG CACCACCAGCAGCAGCAGGCGTCTGGAGGGAATGATAGCAACGCCGCCGTGCTGCCCACTGACCCTCTCA CGCCCTCACTCCATGGGGAGGGTGCTACGCAGCAGCAGCAAAACAGAGGCCAGAACCGTGATGCCCCTCA ACCCTATGAACTCAACACAGCCATCAACTGCAGGGATGAAGTTGTGTCTCCCCTTCCATCTGCCCTGCAG GGTTCCTCAGGCTCCCTTTCTGCCCCTCCAGCTGCCTCAGTTACCTCTGCACCCTCTACTTCTTCCTCCT CCCGACATCGCAAACGTCGCAGGACTTCCAGCAAGTCAGAGGCAGGGGCTAGGGGTGGAAGCCAGGGTTC CAAGGAAAAGGGCAGAGGGAGTGGAGGAGGCCGTCACCACCACCACCCACTACCTGCAACTGGCTTCAAA AAGCAACAGCTAAAGTTCCAATATGGGAATTACTGCAAGTACTATGGCTATCGCAATCCTTCCTGTGAGG ATGTGCGCCTTAGGGTGTTGAAGCCCGAGTGGTTTCAAGGCCGGGACGTCCTAGATCTGGGCTGCAACGT TGGCCATCTGACCCTAAGTATTGCCTGCAAGTGGGGCCCAGCCCGCATGGTGGGGCTGGACATTGATCCA CGGCTTATCCACTCTGCTCGCCAAAATATCCGACACTACCTGTCGGAGGAGCTTCGTCTCCAAGCTCAGA CTTCTGAGGGGGACCCAGGGACAGAGGGGGAGGAAGGGACTATCACTGTCCGAAAAAGGAGCTGCTTCCC AGCCTCACTGACAGCCAGCCGGGGTCCCATCGCTGCACCCCAAGTGCCCTTGGATGGAGCGGACACATCT GTCTTCCCCAACAATGTCGTCTTCGTCACGGGTAACTATGTCCTGGACCGAGATGAGCTGGTGGATGCCC AAAGACCGGAGTATGATGTGGTGCTGTGCTTCAGCCTCACCAAGTGGGTGCACCTGAACTGGGGTGATGA GGGGCTGAAGAGAATGTTCCGCCGCATCTACCGGCACCTGCGCCCTGGGGGTATTCTGGTCCTGGAGCCC CAACCATGGTCCTCCTACTGCAAGAGAAAGTCTCTCACGGAAACAATCTATAAGAACTACTTTCGAATCC AGTTGAAGCCAGAACAGTTCAGTTCCTACCTGACATCCCCAGAGGTGGGTTTCTCCAGCTATGAGCTTGT GGCCACACCGAATAACACCTCCAGAGGCTTCCAGCGTCCTGTGTACTTGTTCCACAAGGCTCGATCCCCC AGCCACTAAGTGGCCTTCTGAGAAGTTGTAAAGAGGCTGCCCTTGCTGCTCCTGAGGACCTGGGGGGAAA GGAAATTATTCCAAGGTCTTTCCTAACTCCAAAAAGTTTCTCCTTTTCTTGGATCTGTCAAGAGAGCTTT TCCTAAGTTGCTACCCCACATCTCCTCCCTGTCACCTCTGGCACTTGAGCAAGCCCAGCTGTGCTGGAAT TCCATCAGCTTCCTCTCCTAGCCCGCTGGGCTGGATGGCATGGACTGTTTGCTGACTCTGCCCACCTGGG ACAGGGGAAGGGGGAATGTCGAGTTCTCCAGGCCCTTCCTCCTGTTCTCAGGGGACTTCCCGCTTCTCCT CAGCCTTTGTTCCTAGCTGCACTTAAATGGACCATGGATGGACTAAGGGTGTTAGAGGGGAAGTTTGGTA AGGAGACACACAGGTACTGTGAAAGTCCTTTTGCTGTCCCCCGGTGGGAGGGAGCTGGGTTTGGAAATGT GAGAACAGCACAATAAACTTGATAACTAGGAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_144913
• Insert Size: 1998 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC026876, AAH26876
• RefSeq Size: 2640 bp
• RefSeq ORF: 1998 bp
• Locus ID: 231803
• UniProt ID: Q8K3A9
• Cytogenetics: 5 76.7 cM
• Summary: S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.[UniProtKB/Swiss-Prot Function]