Rfx3 (NM_011265) Mouse Untagged Clone

SKU MC203934

Rfx3 (untagged) - Mouse regulatory factor X, 3 (influences HLA class II expression) (Rfx3), transcript variant 1, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Rfx3
• Synonyms: C230093O12Rik; MRFX3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC017598; CAACCGTTGCCTTGTGAATCAAGCGCCATAGTCACCGTAGTCCTGGCGACTGTTACCCATCAACAACAAC TACTCCTCTCCTCCTCCTCCTCCTCTTCCTCCTCCTCCTCCTCCCCACCACCACCATCTCCATCACCCAC CAACAACACCACAATAATCCACAGCCAAGAGACCATCATGCAGACTTCAGAGACGGGTTCAGACACAGGT TCGACAGTGACTCTGCAGACGTCTGTGGCTAGCCAAGCAGCAGTGCCTACACAGGTGGTACAGCAAGTGC CAGTGCAGCAGCAGGTGCAGCAGGTACAGACAGTTCAGCAGGTCCAACATGTCTACCCAGCTCAGGTGCA GTATGTGGAAGGAAGTGATACTGTCTATACCAATGGAGCAATCCGAACAACAACTTATCCCTACACAGAA ACACAGATGTACAGCCAAAACACTGGAGGAAATTACTTTGATACTCAAGGAAGTTCTGCCCAGGTGACAA CTGTGGTGTCCTCCCACAGTATGGTGGGTACTGGTGGGATTCAGATGGGCGTCACAGGAGGACAACTCAT CAGCAGCTCGGGAGGAACCTATTTGATCGGCAATTCAATGGAGAACTCTGGTCACTCAGTGACACACACA ACTCGGGCCTCCCCAGCAACAATTGAAATGGCGATTGAGACGCTGCAAAAGTCTGACGGTCTGTCCACTC ACAGAAGCTCTCTTCTCAACAGCCATCTGCAATGGCTGCTGGACAATTATGAGACAGCGGAGGGAGTGAG CCTGCCGAGGAGCACGCTGTACAACCACTACCTTCGACACTGCCAGGAGCACAAGCTGGACCCGGTCAAT GCCGCCTCTTTCGGAAAACTAATAAGGTCCATTTTTATGGGGCTACGAACTAGGAGATTAGGCACTAGAG GAAACTCCAAATACCATTACTATGGGATTCGTGTGAAGCCAGATTCTCCTCTTAACCGTCTACAAGAAGA TATGCAGTACATGGCTATGAGGCAGCAGCCCATGCAACAGAAGCAAAGGTACAAGCCTATGCAGAAAGTG GATGGGGTTGCAGATGGTTTCACAGGAAGTGGCCAACAGACAGGCACATCTGTTGAGCAAACTGTAATTG CCCAAAGTCAACATCATCAACAGTTTTTAGATGCATCTCGAGCACTTCCAGAGTTTGGAGAAGTTGAAAT CTCTTCTCTGCCAGATGGTACTACCTTTGAGGATATCAAGTCACTGCAGAGTCTTTATCGAGAGCACTGT GAGGCAATACTGGACGTTGTTGTGAATCTTCAGTTTAGCCTGATAGAAAAACTGTGGCAAACATTCTGGC GCTATTCCCCATCTACTCCAGCCGACGGCACTACCATCACGGAATCAAGCAATCTGAGTGAAATAGAAAG TCGACTTCCGAAAGCAAAGCTGATAACTCTGTGCAAACATGAGTCTATCCTGAAATGGATGTGTAACTGT GACCATGGGATGTACCAGGCTTTGGTGGAGATTCTCATCCCCGACGTCCTTAGACCCATTCCTAGTGCCT TGACCCAAGCCATTCGAAATTTCGCAAAAAGCCTAGAAGGTTGGCTTTCAAATGCCATGAACAATATTCC ACAGAGAATGATTCAAACCAAGGTTGCCGCTGTAAGTGCCTTTGCTCAGACTCTGCGAAGATACACATCG CTCAATCACCTGGCCCAGGCAGCCCGCGCAGTGCTTCAGAACACTTCCCAGATCAACCAGATGCTCAGTG ACCTCAACCGCGTTGACTTTGCCAATGTCCAGGAGCAGGCTTCCTGGGTGTGCCAGTGTGATGACAACAT GGTTCAGAGACTAGAAACAGACTTCAAGATGACCCTGCAGCAGCAGAGCACTCTGGAGCAGTGGGCTGCT TGGCTGGACAATGTGATGATGCAAGCGCTGAAGCCCTATGAAGGAAGGCCCAGCTTCCCGAAGGCTGCTC GGCAGTTTCTGCTCAAGTGGTCTTTCTACAGCTCCATGGTTATCCGGGACTTAACCTTGCGCAGTGCTGC TAGCTTTGGCTCCTTTCACCTGATCCGGCTGCTCTATGATGAGTACATGTTCTACTTAGTAGAGCATCGT GTTGCTCAAGTGACAGGAGAGACACCGATAGCAGTGATGGGTGAGTTTGGCGATTTAAATGCTGTATCTC CCGGAAATCTGGATAAAGATGAAGGCAGCGAAGTCGAAAGTGAGACGGATGAAGATCTGGATGATTCTTC AGAGCCTCGGGCCAAAAGGGAGAAAACGGAGCTGAGCCAGGCGTTCCCAGTAGGCTGCATGCAGCCTGTT CTGGAGAGTGCTGTGCAGCCCAGCCTCCTGAACCCCCTACACAGCGAGCACATCGTCACAAGTACACAGA CCATCAGGCAGTGCAGCGCCACAGGCAACACCTACACTGCAGTCTAACAGCAGGAAAGCAGAGTTTTCCA GCTTACTCTAACCCCATTGATACTGGGCTTAAGCTGAAATTTTAACAAGCCTGAAGGTTGACTGTTGTCA AAAGTCTATCTGTGCTGAACATTACCTTTTTGGAGTTGTGAAATGGAATGGGTGTATGTATTTTTCCAGG TCTTTTTTTTTTTTTAACACAAATTATTTGCCTTTTAATAATAGAGCCCCTCCCCCCTTAGTTTCAGGTG TCAAGAAGGATTTATACAACACTTAATGTCATTGTTTTTGTTTTCTCATAATTAAAAAGTAATTTACATT TTGTAGCTTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011265
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC017598, AAH17598
• RefSeq Size: 2754 bp
• RefSeq ORF: 2250 bp
• Locus ID: 19726
• UniProt ID: P48381
• Cytogenetics: 19 C1
• Summary: Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells (PubMed:15121860, PubMed:19671664). Also required for ciliated ependymal cell differentiation (PubMed:16930429). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells (PubMed:17229940). Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes (By similarity). Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5). Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells.[UniProtKB/Swiss-Prot Function]