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Gclc (NM_010295) Mouse Untagged Clone

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SKU
MC203908
Gclc (untagged) - Mouse glutamate-cysteine ligase, catalytic subunit (Gclc), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $720.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gclc
Synonyms D9Wsu168e; Ggcs-hs; GLCL-H; Glclc
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC203908 representing BC019374.
Blue=ORF Red=Cloning site Green=Tag(s)

AGCGGAGCTGCGGCCGGGAGGAGGACGCGGCCATGGGGCTGCTGTCCCAAGGCTCGCCACTGAGCTGGG
AAGAGACCCAGCGCCACGCCGACCACGTGCGGAGGCACGGCATCCTCCAGTTCCTGCACATCTACCACG
CAGTCAAGGACCGGCACAAGGACGTGCTCAAGTGGGGTGACGAGGTGGAGTACATGTTGGTGTCCTTCG
ATCATGAAAATAGGAAAGTCCAGTTGTTACTGAATGGAGGCGATGTTCTTGAGACTCTGCAGGAGAAGG
GGGAGAGGACAAACCCCAACCATCCGACCCTCTGGAGACCAGAGTATGGGAGTTACATGATCGAAGGAA
CGCCTGGCCAGCCCTACGGAGGAACGATGTCTGAGTTCAACACTGTGGAGGCCAATATGAGGAAACGCC
GGAAGGAGGCTACTTCTGTACTAGGAGAGCATCAGGCTCTCTGCACCATCACTTCATTCCCCAGACTAG
GCTGTCCTGGATTCACACTGCCAGAACACAGACCCAACCCAGAGGAAGGAGGCGCATCAAAGTCCCTCT
TCTTTCCAGATGAAGCCATAAACAAGCACCCCCGCTTCGGTACTCTAACAAGAAACATCCGGCATCGGA
GAGGAGAAAAGGTTGTCATCAATGTGCCAATATTCAAGGACAAGAATACACCATCTCCATTTGTAGAAA
CATTTCCAGAAGATGCGGAGGCATCAAAGGCTTCTCAGCCAGACCATATCTACATGGATGCCATGGGAT
TCGGGATGGGCAACTGCTGTCTCCAGGTGACATTCCAAGCCTGCAGCATATCTGAGGCAAGATACCTTT
ATGATCAGCTGGCCACTATCTGCCCAATTGTTATGGCTTTGAGTGCTGCATCTCCATTTTACCGAGGCT
ACGTGTCAGACATTGATTGTCGCTGGGGAGTGATTTCTGCCTCTGTAGATGATAGAACACGGGAGGAGA
GAGGCCTGGAGCCTCTGAAGAACAATCGCTTTAGGATCAGTAAGTCTCGGTATGACTCAATAGATAGCT
ACCTGTCTAAGTGTGGGGAGAAGTACAATGACATCGACCTGACCATCGATAAGGAGATCTACGAGCAGC
TCCTGGAGGAAGGCATCGATCATCTTCTGGCACAGCACGTTGCTCATCTCTTTATTAGAGACCCACTCA
CCCTTTTTGAAGAGAAAATACACCTGGATGATGCCAACGAGTCTGACCATTTTGAGAATATTCAGTCTA
CAAACTGGCAGACAATGAGGTTTAAGCCTCCTCCTCCAAACTCAGATATTGGATGGAGAGTAGAGTTCC
GACCAATGGAGGTGCAGTTAACAGACTTTGAGAACTCTGCCTATGTGGTATTCGTGGTACTGCTCACTA
GGGTGATCCTCTCATACAAACTAGACTTCCTCATTCCGCTGTCCAAGGTTGACGAGAACATGAAAGTGG
CCCAGAAGCGAGATGCTGTCTTGCAGGGGATGTTTTATTTCAGGAAAGACATCTGCAAAGGCGGCAACG
CTGTGGTGGACGGGTGCAGCAAGGCCCAGAGCAGCTCTGAGCCAGCTGCAGAGGAGTACACCCTGATGA
GCATAGACACCATCATCAATGGGAAGGAAGGGGTATTTCCTGGACTCATCCCCATTCTGAACTCCTATC
TGGAGAACATGGAAGTTGATGTGGACACCCGATGCAGTATTCTGAACTACCTAAAGCTAATTAAGAAGA
GAGCATCTGGAGAACTAATGACTGTTGCCAGGTGGATGAGAGAGTTCATTGCAAACCATCCTGACTACA
AGCAAGACAGTGTAATAACTGATGAAATCAACTATAGCCTCATTTGGAAGTGCAACCAAATTGCAGATG
AGCTGTGTGAATGTCCAGAGTTACTTGGATCAGGATTTAGAAAAGCGAAGTACAGTGGAGGTAAAAGCG
ACCCTTCAGCCTAGCCTTCAGCCAGCAGAGAGAGAGGGTGATCGCTGTTCAGGATGGCGTGTGCAGCCC
TGACTCCTGGCCCGCTCGGACGCTGGGAAGACCAAGATGACCTGCAGTGTTTCTGCGCCAGTTGCCTGC
AAGTGGCTACTAGAAGCTCCCTTCAGTAGGTAAATCTGAGTTTGTACATGTATATACTAAAGTATTTTT
ATGATTAACAGTGTATTTTAATAACATTGTATCTAAAGTCATAGTGAACTGGCTTGTACATTTTGAATT
CTTACTCTGGAGCAACTACTGTCTAAGCAGTTTTGTAAATGTAATTGTACAATAATACCGGCATTCCAG
AGTTTCAAATGTTTACTGTAAATCTTCCTTTAATACTACCTGGGACCTGATTCACTGAAATTTACTTTA
AAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_010295
Insert Size 2360 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010295
RefSeq Size 2360 bp
RefSeq ORF 2360 bp
Locus ID 14629
UniProt ID P97494
Cytogenetics 9 43.36 cM
MW 88.4 kDa
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Documents
Resources
"NM_010295" in other vectors (4)
SKU Description Size Price
MG209658 Gclc (tGFP-tagged) - Mouse glutamate-cysteine ligase, catalytic subunit (Gclc) 10 ug
$794.00
MR209658 Gclc (Myc-DDK-tagged) - Mouse glutamate-cysteine ligase, catalytic subunit (Gclc) 10 ug
$289.00 $594.00
MR209658L3 Lenti ORF clone of Gclc (Myc-DDK-tagged) - Mouse glutamate-cysteine ligase, catalytic subunit (Gclc) 10 ug
$894.00
MR209658L4 Lenti ORF clone of Gclc (mGFP-tagged) - Mouse glutamate-cysteine ligase, catalytic subunit (Gclc) 10 ug
$894.00

file-alt Citations

Increased glucose availability sensitizes pancreatic cancer to chemotherapy
Vaziri-Gohar, Hue, Abbas et al
Nat Commun (2023) 14 (1), 3823 DOI: 10.1038/s41467-023-38921-8
Cited by 41
AI Summary: OriGene’s GCLC overexpressing plasmids (mouse no. MC203908 and human no. SC119206) were used in this study to investigate how restoring GCLC expression impacts the sensitivity of pancreatic cancer cells to chemotherapy under high glucose conditions.
Snippet: experiments, cell-permeable GSH (L-Glutathione reduced, Sigma, G6013), NAC (N-Acetyl-L-cysteine, Sigma, no. A9165), BSO (L-Buthionine-sulfoximine, Sigma, no. B2515), and GCLC overexpressing plasmids (Origene, mouse (no. MC203908), human (no. SC119206)) were used. Chemotherapies included gemcitabine (gemcitabine hydrochloride, Sigma, no. G6423), oxaliplatin (Sigma, O9512), irinotecan (Sigma, no. I1
FigureFigureFigure
Neuroprotective activity of ursodeoxycholic acid in CHMP2BIntron5 models of frontotemporal dementia
West, Ugbode, Fort-Aznar et al
Neurobiol. Dis. (2020) 144, 105047 DOI: 10.1016/j.nbd.2020.105047
Cited by 24
AI Summary: OriGene’s GCLC (MC203908) and GCLM (MR225622) cDNA products were used to study the roles of glutamate-cysteine ligase subunits in neuronal models of frontotemporal dementia, enabling the investigation of UDCA’s neuroprotective effects in the context of CHMP2BIntron5-induced dysfunction.
Snippet: … The cDNAs for catalytic (GCLC, MC203908) and modifying (GCLM, MR225622) subunits of GCL were obtained from Origene. … Immediately following transfection, cells were washed and incuba;Neurons were transfected at 12 days in vitro (DIV) withLipofectamine 2000 (11,668,019, Thermo Scientific) in transfectionmedium (Suman et al., 2016) with either FLAG-tagged CHMP2BWildtypeor CHMP2BIntron5for 5 h. The cDNAs for catalytic (GCLC, MC203908)and modifying (GCLM, MR225622) subunits of GCL were obtained fromOrigene. …
Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in aging diabetic mice hearts during exercise
Golbidi, Botta, Gottfred et al
Br. J. Pharmacol. (2014)
Cited by 23
AI Summary: OriGene’s mouse GCLc (glutamyl cysteine ligase catalytic subunit) plasmid was used to overexpress GCLc in NIH-3T3 fibroblasts and H9c2 cardiomyocytes, enabling the study of how increased glutathione synthesis affects cell death pathways under oxidative stress in this research.
Snippet: Overexpression of the catalytic subunit 188 of glutamyl cysteine ligase (mouse GCLc, Origene, MD, USA;rat GCLc;Transomic 189 Technologies, AL, USA) in both NIH-3T3 and H9c2 were achieved using Attractene (Qiagen). 190 ... Cite SaveSaving
Showing 1-3 of 3 papers.
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