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Dimt1 (NM_025447) Mouse Untagged Clone

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SKU
MC203899
Dimt1 (untagged) - Mouse DIM1 dimethyladenosine transferase 1-like (S. cerevisiae) (Dimt1), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dimt1
Synonyms 1500031M22Rik; AV111347; Dimt1l
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC019799
CACGCGGTTCCCGGCTGCTTTCGCTTGGCACAGGGACTCCTCCGAGAAGATGCCGAAGGCCAAGTCCGCA GCGAGTAGCCGCCGGCGGGATCGTCAGGAGCAGCGCCGGGAGCTGAAGCGAGCTGGAGGACTCATGTTCA ACACAGGGATCGGACAACACATTTTGAAAAATCCTCTAATTGTTAACAGCATCATCGATAAGGCTGCTTT AAGACCTACCGATGTGGTGCTGGAAGTTGGGCCTGGGACTGGCAATATGACTGTCAAGCTGTTAGAAAAG GCCAAAAAGGTAGTTGCCTGTGAACTTGATCCAAGGCTAGTAGCTGAACTTCACAAAAGAGTTCAGGGCA CGCCTCTGGCCAGCAAACTCCAGGTCCTGGTGGGAGATGTACTGAAATCAGATTTGCCATTCTTTGATGC TTGTGTGGCAAACTTGCCTTATCAGATCTCCTCTCCCTTTGTCTTCAAGTTGCTGCTCCACCGGCCATTT TTCAGATGTGCTATACTTATGTTTCAGAGAGAGTTTGCTCTTCGATTGGTTGCCAAGCCTGGAGATAAAT TATACTGTAGACTCTCCATCAACACACAGCTTTTAGCACGCGTGGACCATCTAATGAAGGTGGGGAAGAA TAACTTCAGACCGCCCCCCAAGGTGGAGTCCAGCGTGGTCAGGATAGAACCTAAGAATCCGCCACCACCA ATCAATTTTCAGGAATGGGACGGCTTAGTAAGGATCACTTTTGTTCGGAAAAACAAGACACTGTCCGCTG CATTCAAATCAAGTGCAGTACAACAACTGTTGGAAAAAAATTACAGAATTCACTGTTCAGTACAGAATAC TGTAATACCAGAAGATTTCAGTATAGCAGATAAAATACAGCAAATCCTAACCAGCACAGGTTTCAGTGAC AAACGGGCCCGTTCCATGGACATAGATGACTTCATCAGGTTGCTACATGGATTCAATGCAGAAGGCATCC ACTTCTCCTAGCCATTTGAAAACTATTTTTTCAAGACCAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_025447
Insert Size 942 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC019799, AAH19799
RefSeq Size 1035 bp
RefSeq ORF 942 bp
Locus ID 66254
UniProt ID Q9D0D4
Cytogenetics 13 D2.1
Summary Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity.UniProtKB/Swiss-Prot Function
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"NM_025447" in other vectors (4)
SKU Description Size Price
MG204446 Dimt1 (tGFP-tagged) - Mouse DIM1 dimethyladenosine transferase 1-like (S 10 ug
$500.00
MR204446 Dimt1 (Myc-DDK-tagged) - Mouse DIM1 dimethyladenosine transferase 1-like (S. cerevisiae) (Dimt1) 10 ug
$289.00 $300.00
MR204446L3 Lenti ORF clone of Dimt1 (Myc-DDK-tagged) - Mouse DIM1 dimethyladenosine transferase 1-like (S. cerevisiae) (Dimt1) 10 ug
$600.00
MR204446L4 Lenti ORF clone of Dimt1 (mGFP-tagged) - Mouse DIM1 dimethyladenosine transferase 1-like (S. cerevisiae) (Dimt1) 10 ug
$600.00

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