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Nek2 (NM_010892) Mouse Untagged Clone

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SKU
MC203803
Nek2 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 2 (Nek2), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nek2
Synonyms AA617254; C77054
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC011316
CGAGGGGCTCGGGTTTCGGCGCGGTTGGCCTGCAGCGGGCGAGCCTGGGCTCCGAGCGGCGCGGTGGTGT GGTTGCCAGGGTGGCCGCTCGCCATGCCGTCCCGGGTGGAGGACTACGAGGTGCTGCACAGCATCGGCAC CGGCTCCTACGGCCGCTGTCAGAAGATTCGGAGGAAGAGCGACGGCAAGATCCTGGTGTGGAAAGAGCTT GACTATGGCTCCATGACGGAGGTGGAGAAGCAGATGCTTGTGTCTGAAGTGAACTTGCTTCGGGAGCTGA AACATCCAAACATCGTCCGTTACTATGATCGCATTATTGACCGAACCAACACAACCCTGTACATCGTAAT GGAATACTGTGAGGGAGGGGACCTGGCTAGTGTCATTTCAAAGGGGACCAAGGATAGACAGTACTTGGAA GAAGAGTTTGTCCTTCGAGTGATGACTCAGTTGACGCTGGCCCTGAAAGAGTGTCACAGAAGGAGCGATG GTGGCCACACTGTGCTTCACCGGGACCTGAAGCCAGCCAATGTCTTCCTGGACAGCAAACACAATGTCAA GCTGGGGGACTTTGGACTAGCTAGAATATTAAATCACGACACGAGTTTTGCAAAAACGTTTGTTGGCACA CCCTATTACATGTCTCCTGAACAGATGAGCTGCTTATCCAGTAACGAGAAGTCGGACATCTGGTCCTTGG GCTGCCTGCTGTATGAGCTGTGTGCACTAATGCCTCCCTTTACAGCTTTCAACCAAAAAGAGCTAGCTGG GAAAATCAGGGAAGGGAGGTTCAGGCGCATCCCCTACCGCTACTCTGATGGCTTGAATGACCTCATCACT CGGATGCTGAATTTAAAGGACTACCATCGACCTTCAGTGGAAGAAATTCTGGAGAGCCCTTTGATAGCAG ACATGGTTGCAGAAGAGCAAAGGAGAAATCTGGAGAGGAGAGGACGGCGCTCAGGCGAGCCTTCGAAGCT GCCGGACTCCAGCCCTGTGCTGAGCGAGCTCAAGTTGAAGGAAAGGCAACTGCAGGATCGAGAGCAAGCA CTCAGAGCTCGGGAGGACATTCTGGAGCAGAAGGAACGTGAACTTTGTATTCGAGAGAGACTTGCAGAGG ACAAACTGGCCAGAGCCGAGAGCCTGATGAAGAACTACAGCCTGCTGAAGGAGCACAGGCTCCTATGTCT GGCTGGTGGCCCAGAACTTGATCTTCCATCCTCAGCCATGAAGAAGAAGGTTCATTTCCACGGGGAAAGC AAAGAGAACACCGCAAGGAGTGAGAATTCTGAGAGCTACCTTGCCAAGTCCAAGTGCAGGGACCTGAAGA AGAGGCTTCATGCTGCCCAGCTGCGGGCTCAAGCCCTGGCTGATATTGAAAAAAACTACCAGCTAAAGAG CAGGCAGATCCTGGGCATGCGCTAGGCCGGCAAGGCATGGAGCTGGGTCAGTGTTGATACTGACAACCCA CTAGAGATTGGTATTCAGCTGCTGTCGTTTTGTGTGTCTGGTTCTGTGGGCAGGAACCTTTGTGTGTGGT GAGCTCGTGGCATTGCTTGTGGTCTGCAAATGGATGTGTGTGTCTGCTTCCTAATGTCCCTGTGTGAAAA GCAAGCTGTCTTTGCTGGTTGGTTGGGCTTTTGATCCTGTGTGTGATTACTACTTGGAATATGAGATGGG GCACTATAGATACTATAGATCTTAGGAGAAAATAATGTTAGGGAAAGAATATTTAACCTGGAGGGCTCAG AGAGGCTCACACGCTGAGTGATACCGCCTTCACTGTGGTTTCCAAGTGAGGCCTCAGCGGGTCCATGACC TCACTGCTAACGGGATGCGTATGGCAGGACCCACAGGGTTGCATGTCAGAGGTGTTGTAATGTTACAGGG AGAGACCCAGCCTCTGGCCTGACCTCCTGATGAGAAGCCACTCACCAGCATTCCCATTCCAGGACGCTCA CTGTGCCAGTTAACATCAGATTAGAAGTGGAGGGGAGGGGAGGGCGAGGGCTGAAGCTTTTAGGATTTGC CTTAGGGAAGCGCGTGCCATGGCCCTCAGCACGCTGCTGTCCTGCATCCTAGGCAGGCACTGGGGACAGG AGGCTCTGGGAGCCCTGAGAGCCCCCTCCTGCTGTGTGCAGGGCCTGTCCCCGTCTGTCTGTATCTGACC TCTAGGTCAGTTGATGACATTAGTTAAGTGTTTCTGTACCTACAAGTATAAGCCAAAAGGTCGGAAATGC CTCAGAGTCACACATGTGGTCCCCAAACATTTAATTTCTGAAGAGAACTGTCTTCAGAAAGTTGGTCTAT TTAAGGTGACTGGGGAAAAGGCAGGTTTCCTTTGCTCTCTGTCCACAGTCCCTTGAGATGGCTTCAGAAG AGAACAGTGTTCAGTGTGCGTTATTGAGGGCCCTGTATGCCTTCACCTGTGTGAGGGCCAGATTGCTGTT TGTCTACGGTTTCTTGAAGACCTCAGCTCAGCAGAAAGGAGTTCATTTGCCCTAACAAGTACTGTTGGCT GAGAGGGGAGGTCAGAAGCATGTAATGCCATTCGTTTCATGGCTAAACACACAGACTCCTTGCATTTTAA AATTCTGTGCCTTTACTTCCTGGAGCTATAATAACGTGCTTGGGTGTGTGACTTGCCGAGGTTGGCTTTC GCAATGCTTTATGTAAACTCTGCTGTTGGCACTTGCAGCTGTAGAAGCTGTCGGAGGGATTACTTACTTA CGGTTATAGCATCTGGGGTCTCTTGCAGCTGCAATGCCTGGGATCTTTTGCAACTCTGACTTTTTCAGTA GCAGCTTGGAATGTTAAAATATGGTGAGCCTCTTCTCTGTGAAGGAAGGGCCTGACAATCTGCCAGCAGG GAAAAGACTGGGAACTCAGTGCAGTGGTTTTCCTGCCTTTCCCTTCTCCTTTGGTCTATAGTGATACTGA CAGCAATACAAAGTAAAATCTGTGTATATAAAAATCCTAAGTATGAGGTCTTAAATGTTCTGGAATTTTA GAAAAAACTTAATTGGATGCAATTGTTTATAATTAACAAGTCATCTTATTTATAAATTTTCCAATATAAT ATAAATCTATTTTGTGGAAATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_010892
Insert Size 1332 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC011316, AAH11316
RefSeq Size 3131 bp
RefSeq ORF 1332 bp
Locus ID 18005
UniProt ID O35942
Cytogenetics 1 96.94 cM
Summary Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (By similarity). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (By similarity). Phosphorylates and activates NEK11 in G1/S-arrested cells. Involved in the regulation of centrosome disjunction (By similarity).UniProtKB/Swiss-Prot Function
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"NM_010892" in other vectors (4)
SKU Description Size Price
MG207063 Nek2 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 2 (Nek2) 10 ug
$657.00
MR207063 Nek2 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 2 (Nek2) 10 ug
$289.00 $457.00
MR207063L3 Lenti ORF clone of Nek2 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 2 (Nek2) 10 ug
$757.00
MR207063L4 Lenti ORF clone of Nek2 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 2 (Nek2) 10 ug
$757.00

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