Dhfr (NM_010049) Mouse Untagged Clone

SKU
MC203752
Dhfr (untagged) - Mouse dihydrofolate reductase (Dhfr), (10ug)
$300.00
In Stock*
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dhfr
Synonyms 8430436I03Rik; AA607882; AI662710; AW555094
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC005796
CGGCAATCCTAGCGTGAAGGCTGGTAGGATTTTATCCCCGCTGCCATCATGGTTCGACCATTGAACTGCA TCGTCGCCGTGTCCCAAAATATGGGGATTGGCAAGAACGGAGACCTACCCTGGCCTCCGCTCAGGAACGA GTTCAAGTACTTCCAAAGAATGACCACAACCTCTTCAGTGGAAGGTAAACAGAATCTGGTGATTATGGGT AGGAAAACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGACAGAATTAATATAGTTCTCAGTA GAGAACTCAAAGAACCACCACGAGGAGCTCATTTTCTTGCCAAAAGTTTGGATGATGCCTTAAGACTTAT TGAACAACCGGAATTGGCAAGTAAAGTAGACATGGTTTGGATAGTCGGAGGCAGTTCTGTTTACCAGGAA GCCATGAATCAACCAGGCCACCTCAGACTCTTTGTGACAAGGATCATGCAGGAATTTGAAAGTGACACGT TTTTCCCAGAAATTGATTTGGGGAAATATAAACTTCTCCCAGAATACCCAGGCGTCCTCTCTGAGGTCCA GGAGGAAAAAGGCATCAAGTATAAGTTTGAAGTCTACGAGAAGAAAGACTAACAGGAAGATGCTTTCAAG TTCTCTGCTCCCCTCCTAAAGCTATGCATTTTTATAAGACCATGGGACTTTTGCTGGCTTTAGATCTATG AGTAATTATTTCTTTAGGGAGGGGTAGTTGGAAGAATTGTTTGTTTTGTGATCCTGGGGATGGAACCTAA GACCCAGTGCGTGCTGAGCAAATGCTATACTGCTGAGCCACCCCAACCCTAGCCCCTATATAATTCTAAA CAATATGTTGTCATTTCCCAGTAATCTAACAAGGTTATAGTAAAAGTGCCTTAAGAAATGTCACTTGCTA TAAAGGTCTCAGTGCCCCTCCCATGAGACCTCAAGTGGCTCCCCAGCATATGCACAGGGTACTGTGTGTA CAAGAGACCCCAGTGATGTAGAGCCCCTGGAGCATGAGCAGATGTGTGGGCTCATAAAAGTAGGAGCTAG GCAGGTAAGTCCAAAGGGCAGAAACAGGTTTTAAACAGCAGAGCTGGAACTCAGACTATAAAGAAAATTC CATCAAAGTAGAGACTGGATTATTGTATGCACATCACACTTGCAGCAAAGCTCTGCTCACTCAGACAGAA AATCAGTAAATGGAGAACTCCATTGTGTTCCATGGAGACGAGAGCAGGTGGAAGATTATGTAAGATCTGA AACACTGAAATTGTCTGCTTCTCATCTTCAGTGAGATTCCAAAGGATAGTACAGTGACAGAACAAGAATA GGCACTCTCTACAAAAAAAAGAAAGAAAAAACTAAGTAATAGCAAGCATAATAGCTACTGTTAAGAACTC AGAGATAATGAATTGAGAATGGATACTGCTTGAAATGAAAATTTAATAAGTTAGAAACTAAACTTTATAA AAATAAAAAAATGAGCATTAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI
ACCN NM_010049
Insert Size 564 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC005796, AAH05796
RefSeq Size 1510 bp
RefSeq ORF 564 bp
Locus ID 13361
UniProt ID P00375
Cytogenetics 13 47.64 cM
Summary Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway (PubMed:25980602). Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (PubMed:25980602). Binds its own mRNA.[UniProtKB/Swiss-Prot Function]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.