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Supt5 (NM_013676) Mouse Untagged Clone

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SKU
MC203677
Supt5 (untagged) - Mouse suppressor of Ty 5 homolog (S. cerevisiae) (Supt5h), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,414.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Supt5
Synonyms AL033283; AL033321; AU019549; Spt5; Supt5h
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC007132
GGAAGCTTCAGGTGGCAAGCGATCGCTGCGGAAGATGTCGGACAGCGAAGACAGCAACTTCTCCGAGGAG GAGGACAGCGAGCGCAGCAGCGAGGCGGAGGAGGCCGAGGTTGAGGAAGACCAGCGAAGTGCTGCAGGCA GTGAAAAAGAGGAGGAGCCTGAGGAAGAGGAGGAGGAAGAGGAAGAATACGATGAGGAAGAAGAGGAGGA AGATGATGATAGGCCCCCCAAGAAACCACGCCATGGGGGCTTCATTTTAGATGAGGCTGATGTAGATGAT GAATATGAAGATGAAGACCAGTGGGAAGATGGAGCTGAAGATATCCTGGAGAAAGAAGAGATTGAAGCCT CCAATATTGATAACGTTGTGCTGGATGAAGACCGCTCTGGGGCTCGTCGTCTGCAGAATCTCTGGAGGGA CCAGAGAGAAGAAGAACTGGGCGAGTATTACATGAAGAAATATGCTAAGTCGTCTGTGGGAGAGACGGTG TATGGAGGGTCTGACGAGCTCTCAGATGACATCACTCAGCAGCAGCTGCTCCCAGGAGTCAAGGACCCCA ACCTGTGGACTGTCAAGTGTAAGATTGGGGAGGAACGAGCCACGGCAATTTCCTTGATGCGGAAATTCAT CGCTTACCAGTTCACAGACACACCCCTGCAGATTAAGTCAGTGGTTGCCCCTGAGCACGTGAAGGGCTAC ATCTATGTGGAGGCCTACAAACAGACCCATGTGAAGCAGGCCATTGAAGGTGTGGGCAACCTGCGGCTTG GCTACTGGAACCAGCAGATGGTGCCTATTAAGGAGATGACCGATGTACTCAAGGTGGTGAAGGAGGTGGC CAACCTGAAGCCGAAGTCCTGGGTCCGCCTCAAGCGAGGCATCTACAAAGATGACATTGCTCAGGTGGAC TATGTGGAGCCCAGCCAGAACACCATTTCCCTGAAGATGATCCCGCGCATCGACTACGACCGCATCAAAG CCCGAATGAGCCTGAAAGACTGGTTTGCCAAAAGGAAGAAGTTCAAGCGGCCTCCACAGAGGCTCTTTGA TGCAGAGAAGATCAGGTCCTTGGGGGGTGATGTTGCCTCTGATGGTGACTTCCTCATCTTTGAGGGGAAC CGCTACAGCCGAAAGGGCTTCCTGTTCAAGAGCTTTGCCATGTCTGCTGTGATTACAGAAGGTGTGAAGC CCACACTGTCAGAGCTGGAAAAGTTTGAAGATCAGCCTGAGGGCATCGACCTGGAGGTTGTGACTGAAAG CACAGGGAAGGAGCGGGAACACAATTTCCAGCCTGGGGACAATGTGGAGGTGTGCGAGGGCGAGCTCATC AACCTACAGGGCAAGGTCCTCAGCGTGGATGGCAACAAGATCACTATCATGCCCAAGCACGAGGACCTCA AGGACATGCTTGAGTTCCCCGCTCAGGAACTTCGGAAGTACTTCAAGATGGGGGACCACGTAAAAGTGAT TGCTGGCCGATTTGAGGGTGACACAGGTCTCATTGTGCGGGTGGAAGAGAACTTTGTTATTCTCTTTTCA GACCTCACCATGCATGAGTTGAAAGTGCTTCCCCGAGACCTGCAGCTCTGCTCAGAGACAGCCTCAGGCG TGGATGTTGGGGGCCAGCATGAGTGGGGTGAGCTGGTGCAGCTGGATCCCCGTACTGTGGGTGTCATCGT GCGGCTAGAGCGAGAAACCTTCCAGGTTCTCAACATGCACGGGAAGGTGGTGACCGTTAGGCACCAGGCC GTGACGCAGAAGAAGGACAACCGCTTCGCTGTGGCTCTGGACTCGGATCAGAACAACATCCATGTGAAGG ACATTGTCAAGGTCATCGATGGCCCTCACTCAGGCCGAGAGGGTGAGATCCGCCATCTGTACCGCAGCTT TGCCTTCCTGCACTGCAAGAAGCTGGTGGAGAACGGCGGCATGTTTGTCTGTAAGGCACGGCACCTGGTG TTGGCTGGGGGCTCAAAGCCCCGGGATGTGACCAATTTAACTGTTGGTGGCTTCACTCCTATGAGCCCCC GGATCAGCAGCCCTATGCACCCCAGTGCTGAAGGTCAGCATGGTGGCTTTGGAAGCCCAGGTGGCATGAG CAGGGGTCGAGGGAGAAGAGACAATGAACTCATAGGCCAGACTGTGCGTATCTCCCAGGGACCCTACAAA GGCTACATTGGTGTGGTGAAGGATGCCACAGAGTCCACGGCCAGAGTAGAACTGCATTCTACCTGCCAGA CCATCTCTGTGGATCGCCAGCGGCTCACCACGGTCGACTCCCAGCGTCCAGGTGGCATGACCTCTACATA TGGACGGACTCCCATGTATGGCTCTCAGACACCCATGTATGGCTCTGGCTCCCGAACACCTATGTATGGC TCCCAGACACCTTTGCAGGATGGCAGCCGCACTCCACATTATGGCTCACAGACACCCCTGCATGATGGTA GCCGCACTCCTGCTCAGAGTGGGGCCTGGGACCCCAACAACCCAAACACGCCTTCCAGGGCTGAGGAGGA ATACGAATATGCCTTTGACGACGAACCCACCCCATCCCCACAAGCATATGGGGGAACCCCGAATCCCCAA ACACCCGGCTACCCAGACCCATCATCCCCCCAGGTCAATCCACAGTACAATCCACAGACACCAGGGACGC CAGCCATGTACAACACAGACCAGTTCTCCCCCTACGCTGCCCCCTCTCCACAGGGGTCTTACCAGCCCAG CCCCAGCCCCCAGAGCTACCACCAAGTGGCACCAAGTCCAGCAGGCTATCAGAACACCCATTCCCCAGCC AGCTATCACCCGACACCGTCACCTATGGCCTATCAGGCTAGTCCCAGCCCAAGCCCTGTTGGCTACAGTC CAATGACCCCTGGAGCACCTTCTCCTGGAGGTTACAATCCCCACACACCGGGCTCAGGCATTGAGCAGAA CTCTAGCGACTGGGTGACCACTGATATCCAGGTGAAGGTTCGAGACACCTATCTGGACACACAAATAGTG GGGCAGACAGGCGTCATCCGCAGTGTCACGGGAGGCATGTGCTCCGTGTACCTGAAGGACAGTGAGAAGG TGGTCAGCATCTCCAGTGAACATCTGGAGCCCATCACGCCCACCAAGAACAACAAGGTGAAGGTGATTCT GGGTGAGGACCGAGAGGCTACCGGGGTCCTACTGAGCATTGATGGTGAGGATGGCATCATCCGCATGGAC CTAGAGGACCAGCAGATCAAGATTCTAAACCTCCGCTTCCTAGGGAAGCTCCTGGAAGCCTGACGCCCAA GTGTGCAGTGCAGACATGGAGCCAGCAGCCCCTCTGGCCTTGTTTGGCCTAGCATGGAACCCTGAGTCTG GAGCAAGCCAGGGCAATCACTCTGTATTTCCTTTTCCTCCTTCCCTGATACTCTAGGAGTCAGAGCAGAG TCTGAGGAATGTTCCTGCCTTCTTCTGCAGCCCCAGCTTGACTTTGTGTTCAGGTCTTTCAATAAAGAGA AGCTGTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_013676
Insert Size 3249 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC007132, AAH07132
RefSeq Size 3561 bp
RefSeq ORF 3249 bp
Locus ID 20924
UniProt ID O55201
Cytogenetics 7 16.68 cM
Summary Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (By similarity).UniProtKB/Swiss-Prot Function
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Resources
"NM_013676" in other vectors (4)
SKU Description Size Price
MG211605 Supt5 (tGFP-tagged) - Mouse suppressor of Ty 5 homolog (S. cerevisiae) (Supt5h) 10 ug
$1,613.00
MR211605 Supt5 (Myc-DDK-tagged) - Mouse suppressor of Ty 5 homolog (S. cerevisiae) (Supt5h) 10 ug
$1,413.00
MR211605L3 Lenti ORF clone of Supt5 (Myc-DDK-tagged) - Mouse suppressor of Ty 5 homolog (S. cerevisiae) (Supt5h) 10 ug
$1,713.00
MR211605L4 Lenti ORF clone of Supt5 (mGFP-tagged) - Mouse suppressor of Ty 5 homolog (S. cerevisiae) (Supt5h) 10 ug
$1,713.00

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