Bpifa1 (NM_011126) Mouse Untagged Clone

SKU
MC203603
Bpifa1 (untagged) - Mouse palate, lung, and nasal epithelium associated (Plunc), (10ug)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Bpifa1
Synonyms LUNX; NASG; Plunc; SPLUNC1; SPURT
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC054375
GGACGACCACTGAGACCTTGAGACTCAGACACCAAGAGAGATGTTTCTAGTTGGGAGCCTCGTTGTCCTC TGTGGGCTGCTGGCCCACAGCACAGCACAGCTGGCAGGCTTGCCATTGCCCCTGGGCCAGGGTCCACCCT TGCCACTGAACCAGGGCCCACCGTTGCCACTGAACCAGGGCCAGCTGTTGCCCCTGGCTCAGGGTCTGCC TTTGGCTGTAAGCCCAGCACTGCCTTCAAATCCCACAGATCTTCTTGCTGGAAAATTCACAGATGCTCTC AGCGGTGGCCTGCTGTCTGGGGGGCTGCTGGGCATTTTGGAAAATATTCCACTCCTGGATGTTATAAAGT CTGGAGGAGGCAATTCTAATGGCCTTGTTGGGGGCCTGCTGGGAAAACTGACGTCATCAGTTCCTCTCCT GAACAACATCCTCGACATAAAAATCACTGATCCGCAGCTGCTAGAACTTGGTCTTGTGCAGAGTCCTGAT GGCCATCGTCTCTATGTCACCATCCCTCTGGGCTTGACACTCAACGTAAATATGCCCGTAGTTGGAAGTC TTTTGCAATTGGCTGTGAAGCTGAACATTACTGCAGAAGTCTTAGCCGTGAAAGACAATCAGGGGAGGAT TCATCTGGTTCTTGGTGACTGCACCCACTCCCCTGGCAGCCTGAAAATCAGCTTGCTCAATGGAGTCACT CCTGTTCAAAGCTTTTTAGACAACCTCACAGGGATATTGACTAAAGTCCTTCCTGAGCTGATCCAGGGCA AGGTATGTCCTCTGGTCAATGGGATTCTCAGCGGTTTGGATGTCACCCTGGTGCACAACATTGCTGAATT ACTGATCCATGGACTACAGTTTGTCATCAAAGTTTAGGCATCCCAGGAAGGAAGGCTATCTTGGCTGAGC TGAATCATTTCTTGCTGCTCAGTCTCCTGCCTCTTGCCCAGTCTCCCATGGCTCACAGAAAGGGGCCCAC ATCCTGGAAAATTATGTCTTCCTTCTCCTCACGGAGCCTGATCTCTTCCCATCAGGCACGATTAATCCTG TGATCCTCACTAAATAAAATAGCTCTTCATCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAG
Restriction Sites RsrII-NotI |
ACCN NM_011126
Insert Size 837 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC054375, AAH54375
RefSeq Size 1156 bp
RefSeq ORF 837 bp
Locus ID 18843
UniProt ID P97361
Cytogenetics 2 H1
Summary Lipid-binding protein which shows high specificity for the surfactant phospholipid dipalmitoylphosphatidylcholine (DPPC) (By similarity). Plays a role in the innate immune responses of the upper airways (PubMed:23499554). Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae (PubMed:23499554). Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus (By similarity). Plays a role in the airway inflammatory response after exposure to irritants (By similarity). May attract macrophages and neutrophils (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011126" in other vectors (4)
SKU Description Size Price
MG203753 Bpifa1 (tGFP-tagged) - Mouse palate, lung, and nasal epithelium carcinoma associated (Plunc) 10 ug
$500.00
MR203753 Bpifa1 (Myc-DDK-tagged) - Mouse palate, lung, and nasal epithelium associated (Plunc) 10 ug
$289.00 $300.00
MR203753L3 Lenti ORF clone of Bpifa1 (Myc-DDK-tagged) - Mouse palate, lung, and nasal epithelium associated (Plunc) 10 ug
$600.00
MR203753L4 Lenti ORF clone of Bpifa1 (mGFP-tagged) - Mouse palate, lung, and nasal epithelium associated (Plunc) 10 ug
$600.00

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