Gja5 (NM_008121) Mouse Untagged Clone

SKU MC203593

Gja5 (untagged) - Mouse gap junction protein, alpha 5 (Gja5), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Gja5
• Synonyms: 5730555N10Rik; Cnx40; Cx40; Gja-5
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC053054; ATAACAGTGGGCAGTTGAACAGCAGCCAGAGCCTGAAGAAGCCAACTCCAGGGAGGAGGAAAGGAAGCAG AAGGCTCGGCCTCGGTCTCCTACTCTTGGCAAGATGGGTGACTGGAGCTTCCTGGGGGAGTTCCTGGAGG AGGTCCACAAGCACTCCACAGTCATCGGCAAGGTCTGGCTCACTGTCCTGTTCATTTTCCGCATGCTGGT CCTGGGCACCGCTGCTGAGTCCTCCTGGGGAGATGAGCAGGCCGACTTCCGGTGCGATACCATTCAGCCT GGTTGCCAAAATGTCTGCTATGACCAAGCCTTCCCCATCTCCCACATTCGTTATTGGGTACTGCAGATCA TCTTTGTGTCCACGCCTTCTCTAGTGTACATGGGCCATGCCATGCACACTGTGCGCATGCAGGAAAAGCA GAAATTGCGGGATGCTGAGAAAGCTAAAGAGGCCCACCGCACTGGTGCCTATGAGTACCCAGTAGCCGAA AAGGCCGAGCTGTCCTGCTGGAAAGAAGTAGATGGGAAGATTGTCCTCCAGGGCACCCTACTCAACACCT ATGTCTGCACCATTCTGATCCGCACCACCATGGAGGTGGCCTTCATCGTAGGCCAGTACCTCCTCTATGG GATCTTCCTGGATACCCTGCATGTCTGCCGCAGGAGTCCCTGTCCCCACCCAGTCAACTGTTATGTTTCG AGGCCCACGGAGAAGAATGTCTTCATTGTCTTTATGATGGCTGTGGCTGGACTGTCTCTGTTTCTCAGCC TGGCTGAACTCTACCACCTGGGCTGGAAGAAGATCCGACAGCGCTTTGGCAAGTCACGGCAGGGTGTGGA CAAGCACCAGCTGCCTGGCCCTCCCACCAGCCTCGTCCAGAGCCTCACTCCTCCCCCTGACTTCAATCAG TGCCTAAAGAACAGCTCCGGAGAGAAATTCTTCAGCGACTTCAGTAATAACATGGGCTCCCGGAAGAATC CAGACGCTCTGGCCACTGGGGAAGTGCCAAACCAGGAGCAGATTCCAGGGGAAGGCTTCATCCACATGCA CTATAGCCAGAAGCCAGAGTACGCCAGTGGAGCCTCTGCGGGCCACCGCCTTCCTCAGGGCTACCATAGT GACAAACGGCGCCTTAGTAAGGCCAGCAGCAAAGCAAGGTCAGATGACCTGTCAGTGTGATCCTCCTTTT AGGGAGGGCCAGGCCCAGGAGGGATCTAAGGAGTGTGAGGCAGGAAAGAAACAGTCTCTCTCGTGATGGT ATACTCTCCTCAGCACTACTTTGCTCTGTCTGTCCTGGGTCTGGGTGGCATTCATTGGCATTCATTGCTC GTTATCCTCAAAATTACAGTTCTGAGTCTTCTCAATGACTGGGCTACTCCTCAAGTCTCATTCCCTCCTA CTCATCTACCAGTATTCACAGTGAAGCCATAGTGGAGGCAGGATCAGGAACCGTGGCAAAATCTGGTCTG GGGAGGACAGGTTCAACACCAGCAACATACCAGATAGACCCCCCACCCACCCCAAGTCTTCTCTTTGGTA CTATGCCCATCCTCTTGTTTTGTTTTGTTTTGTTTTTTCTTAAGAGCCCAAGGTTGAGAGTATTCATCAA GTAATCTGCCCTGTATCATTCCTTTGAATCAGTTGTTTCCATGTGCCATACCTCGGAGTGCTGGTGAGGT AACTGTGGGCTGCCCTTGGCTGCATCTCCTTCCTCCCAGTCTTAAAGGGGTATCCTTGGTCCTTGGCCAG CATCAGATTTACAAGTGCCTTGGTATGTACCTTTGGCAAATGTTTCATGTTGATGATGTCTCAGCCTTAC TGCCAGGCTAGACACCCAGCTCGTAAGGGTGCCAAGACCTCCAGAAGTCACTGTGCACACAAGCATCGCT GGAATTGCCACCAGCTGTCATCCTGCGGTCCTTTATAGTTTAACCTAATGACAGAAACCATCCTGGCCGG CTCACCCACCGTTCCTCTAAAATCTTTGTTAACAGGAATGTCCAAAGTCACCATCCTCTCTTGGATCCTG GCCAGATTACCAGCCACCAAGTTCAGTGGAATTTCCCCAGGATTTACTGAAACAAAGAGAGCATTGTGCT TCTCGGGTTCTTCTGGGTCTGGGGATGGGGGTGGAGGGGGAAATTCTGCTGTGAAGATGAAAAGGTGGGG GGAGGACACTGAAAAACTATTTTCCCTTCTGTGTATGCCTTCCACTGTCTGCCAGCTTTTAATGCCGAGA GGTGTGGTGATAGCAATTGAGGCCCAAGATCTCTCTTAGAGGCATCAAGGAGACATGGAAGCAGAAAGGG TCCAGTTGGTTTGGACCCTTATTTAGTGCAGATATTCCTATGTTTTCTTGGTACCCCTAGGTACAACAGG GGAGGACTGTAGAAAATCCCTGCCTTCCTCAGGGTCTATCCTGGGGTATCTACTGAGTGGATCCATCTTA TGCAGATGGAAGAAGCTCAGCACCAGATACCGAGATTTACAAACACAATACTGTGTGTGTGTGTGTGTGT GTGTGTGTGTGTGTGTGTTTGTGTGTGCATGCCCATCCATCTCTGTGTGCCTCTGTGTGTGTGTGCTCGT GTGTGCATGCAGATAGAATGGTGAAGAAGAGCCCTGAAGAACGATAAGGAAACATGTAGAGAAACTTGGG TCTTTCTGCTTCCTTCTCAGTTGTCTGGCAGGAGGGCTGAGCTTGCTTCTTAATCAGCCTAAAGATCAGG CATGAGAAGTTCAGGAGGGTGAAGGGTCAGAGGTCAGTCCCTGTCCCAGTGACCCATCTCTGGGTCCCTC TGCTGGCATTTGGTTGCTACTAATGGATAGAAAGAAATTGACTGAGGATACCTGGGAAAGGAAGACGGGC TGTTCCAGTCCCAGTGCATTTCATCCATTCCCGACACTGGCACTAAGACTCTCTGTTCTGGCTATGGAGT GTGTGTTTGTTTTCTGAAATGGGATTTTTTTGAATTAGAAAATCTGAGACCAGTCGTTGATGGTGAGGTT TTTTTTGTTTTCTGTTTTTTTTTTTTTTTTTTTTTCAGAAGAGAAAGAGGAAATACTATGTTTGACTGGT TTTAAATAAAATCTATCTCTACATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_008121
• Insert Size: 1077 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC053054, AAH53054
• RefSeq Size: 3166 bp
• RefSeq ORF: 1077 bp
• Locus ID: 14613
• UniProt ID: Q01231
• Cytogenetics: 3 42.04 cM
• Summary: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.