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Pcbp1 (NM_011865) Mouse Untagged Clone

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SKU
MC203380
Pcbp1 (untagged) - Mouse poly(rC) binding protein 1 (Pcbp1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pcbp1
Synonyms alphaCP-1; hnRNP E1; WBP17; aCP-1
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC069915
CGGACGCGTGGGTGCCGCCGGAGTCGCCACCGCCGCGCCCTCGCCCACCCGCCCGTCCACCGCTCCCGGT CCCGCTCGCCCCCGTCGCCGCCGCCGCCAGTCCTGCGACGCCGCCGCGGCCTCCCGCCCGCGCCCGCCCG CTCCTGCGGCCCCCCGCTCGCCTCGCGCCGGCAGTTCGGGCCTACTCCTCCCCTCCCCCGCCAGCCGCCC AGGACTTGACCACGTAACGAGCCCAACTCCCGAACGCCGCCCGCCGCTCGCCATGGACGCCGGTGTGACT GAAAGCGGACTCAACGTGACTCTCACCATTCGGCTGCTGATGCACGGAAAGGAAGTAGGCAGCATCATCG GGAAGAAAGGGGAGTCGGTGAAGAGGATCCGCGAGGAGAGCGGCGCGCGGATCAACATCTCGGAGGGGAA CTGCCCGGAGAGGATCATCACGCTGACTGGGCCTACCAATGCCATCTTTAAGGCCTTCGCCATGATCATC GACAAGCTGGAGGAAGATATCAACAGCTCCATGACCAACAGTACGGCGGCCAGCAGGCCCCCGGTCACAC TTCGGCTGGTCGTGCCCGCCACCCAGTGTGGCTCCCTGATCGGCAAGGGCGGCTGCAAGATCAAGGAGAT CCGCGAGAGCACCGGGGCCCAGGTCCAGGTGGCGGGGGATATGCTGCCCAACTCGACCGAGCGGGCTATC ACTATCGCCGGCGTGCCGCAGTCCGTCACCGAGTGTGTGAAGCAGATCTGCCTGGTCATGCTGGAGACGC TCTCCCAGTCTCCACAGGGGAGAGTCATGACCATCCCGTACCAGCCCATGCCGGCCAGCTCGCCAGTCAT CTGCGCGGGCGGCCAAGATCGCTGCAGCGACGCGGCGGGCTACCCCCACGCCACCCACGACCTGGAGGGA CCACCTCTAGACGCCTACTCGATTCAAGGACAACACACCATTTCTCCGCTGGATCTGGCCAAGCTGAACC AGGTGGCAAGACAACAGTCTCACTTTGCCATGATGCACGGCGGGACGGGATTCGCCGGAATTGACTCCAG CTCTCCAGAGGTGAAAGGCTATTGGGCAAGTTTGGATGCATCTACTCAAACCACCCATGAACTCACCATT CCAAATAACTTAATCGGCTGCATAATCGGGCGCCAAGGCGCCAACATCAATGAGATCCGCCAGATGTCCG GGGCCCAGATCAAAATTGCCAACCCGGTGGAAGGCTCTTCTGGAAGGCAGGTCACTATTACTGGCTCGGC TGCCAGTATTAGCCTGGCCCAGTATCTAATCAATGCCAGGCTTTCCTCTGAGAAGGGGATGGGGTGCAGC TAGAACAGCGTAGGTTCGCTCGTAACCCCCTTCCTGCTGTTTCCCATGATCCAACTGTGTAATTTCTGGT CGGTAATTCCAGGTTTTAAATAATTTGTAAGTGTTCAGTTTCTACACTTTATCATCCGCTAAGAATTTAA AGAAAAATCACATTCTCTGTTCAGCCGTTAATGCTGGGATCCATATTTAGTTTTATAAGCTTTTCCCTGT TTTTAGTTTCGTTTGGGTTTTTTTGGCTCATGGATTTTATTTTCTGTTTGTCGATAAGAAATGTAAGAGT GGAATGTTAATAAATTTCAGTTTAGTTCTGTAATGTCAAGAATTTAAGAATTAAACAAATGGATTGGTTA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_011865
Insert Size 1071 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC069915, AAH69915
RefSeq Size 1711 bp
RefSeq ORF 1071 bp
Locus ID 23983
UniProt ID P60335
Cytogenetics 6 D1
Summary Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011865" in other vectors (4)
SKU Description Size Price
MG205429 Pcbp1 (tGFP-tagged) - Mouse poly(rC) binding protein 1 (Pcbp1) 10 ug
$657.00
MR205429 Pcbp1 (Myc-DDK-tagged) - Mouse poly(rC) binding protein 1 (Pcbp1) 10 ug
$289.00 $457.00
MR205429L3 Lenti ORF clone of Pcbp1 (Myc-DDK-tagged) - Mouse poly(rC) binding protein 1 (Pcbp1) 10 ug
$757.00
MR205429L4 Lenti ORF clone of Pcbp1 (mGFP-tagged) - Mouse poly(rC) binding protein 1 (Pcbp1) 10 ug
$757.00

file-alt Citations

Maternal PCBP1 determines the normal timing of pronucleus formation in mouse eggs
Shi, Zhao, Yang et al
Cell. Mol. Life Sci. (2015)
Cited by 4
AI Summary: OriGene contributed to this study by providing the full-length mouse Pcbp1 plasmid, which was used for cloning and in vitro transcription experiments to investigate the role of PCBP1 in pronucleus formation.
Snippet: Construction of Pcbp1 plasmid and in vitro transcription of RNA. The plasmid-containing full-length mouse Pcbp1 was purchased from OriGene (MD, USA), and the Pcbp1 coding sequence was amplified and cloned at FseI and AscI of pCS2 + vector. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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