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Sema3e (NM_011348) Mouse Untagged Clone

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SKU
MC203244
Sema3e (untagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $711.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Sema3e
Synonyms 6430702L12; AA408817; mKIAA0331; Semah
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC057956
GGCGGCATCCCCGCTGGGTGGATCCCGCGCTCCTGGCGCAAGTGGCACTTCTTGCTTCTAATTATCGAGA GGAGAGGCGAATACGAACTAGCTGCTCGGCAAGTCAGTGCAGGAGGCTGACTTCTGGGAGGCTGGCGGGG AGGCTGGGGGAAGAGCTGGGGGAGGCTGCTGCTCTGCTCCGACTGTTTTCTCAATGAATAGCTGGCGGGG AGACTGAAGCTAGCCACAGCCTCCTCCTCACTCCGCGTCTGGGTGACGGCGACAGCACCAGCCCGGACCT GGCTCTCAAGACGCGCTCCTTGGACGGTCTCTTGCTCCGCGCTTCTAACCACCGGGCCCAAGGACAGAAA GGCTTAGCGGATCCAAATATTGCCCGGCAAATGGCACTTGGGAATGGTATTTTCTGATGACAACCCCTTC TGTTTGTGACAAAGCCTGTCGCCCGCAGTTGCCCCTGGAGGGAAGTACTAAGTAAAACTCAATCCTGTCT TAAAGTGTGGCTGCAGGGGCCAGAGGAGAGCCAGCACGCACCATGGCACCGGCCGGACACATCCTCACCT TGCTGCTCTGGGGTCACCTGCTGGAACTCTGGACCCCAGGTCACTCCGCGAACCCCTCCTACCCCAGGCT ACGCCTGTCACATAAAGAACTTTTGGAACTGAATAGGACTTCAATATTTCAAAGCCCCCTTGGATTTCTT GATCTCCATACAATGCTGCTGGATGAGTATCAAGAACGGCTCTTTGTGGGAGGCAGAGACCTTGTCTATT CCCTGAACTTGGAACGAGTCAGTGACGGCTACAGAGAGATATACTGGCCGAGCACAGCAGTAAAGGTAGA AGAATGCATAATGAAAGGAAAAGACGCAAATGAGTGTGCCAATTATATCCGGGTTTTGCATCACTACAAC AGGACACACCTTCTGACCTGTGCTACTGGAGCTTTTGATCCACACTGTGCCTTCATCAGAGTCGGGCACC ATTCAGAGGAACCCCTGTTTCACCTGGAGTCACACAGATCTGAGAGAGGAAGGGGCAGATGTCCTTTTGA CCCCAACTCCTCCTTTGTGTCCACGCTAGTTGGGAATGAGCTGTTTGCTGGACTCTACAGTGACTATTGG GGCAGAGACTCGGCGATCTTCCGCAGCATGGGGAAGTTAGGCCATATTCGCACTGAGCATGACGATGAGC GGCTCCTGAAAGAACCAAAATTTGTAGGTTCATATATGATTCCTGATAACGAAGACCGAGATGACAACAA AATGTACTTTTTCTTTACTGAGAAGGCGCTGGAGGCGGAGAACAACGCCCACACGATCTACACCCGAGTG GGGCGGCTGTGCGTGAATGACATGGGAGGACAGAGAATCCTGGTGAACAAGTGGAGCACTTTCCTTAAAG CGCGGCTGGTTTGCTCAGTGCCGGGAATGAATGGAATCGACACATACTTTGACGAACTAGAGGATGTGTT TTTACTGCCGACCAGAGATCCTAAGAATCCAGTGATATTTGGACTGTTTAATACTACCAGCAATATATTT AGAGGCCATGCTGTATGTGTGTATCACATGTCAAGTATCCGGGAAGCCTTTAATGGCCCATATGCTCATA AAGAAGGCCCTGAATACCACTGGTCACTATATGAAGGAAAAGTCCCCTACCCAAGGCCTGGTTCCTGTGC CAGCAAAGTAAACGGAGGCAAGTATGGAACCACCAAAGATTACCCCGATGACGCCATCCGGTTCGCAAGG ATGCATCCTCTAATGTATCAGCCCATAAAACCTGTTCATAAAAAACCAATACTGGTAAAAACAGATGGAA AATACAACCTGAGGCAACTTGCCGTGGATCGGGTGGAAGCGGAGGATGGCCAGTATGACGTCTTATTTAT TGGGACAGACACAGGAATTGTGCTGAAAGTAATCACAATTTACAACCAAGAAACAGAGTGGATGGAGGAA GTCATTCTAGAGGAACTTCAAATATTCAAGGATCCAGCCCCTATCATTTCTATGGAAATTTCTTCAAAGA GACAACAGCTTTACATTGGATCAGCCTCTGCTGTGGCACAAGTCAGATTCCATCACTGCGACATGTATGG CAGTGCTTGTGCTGACTGCTGCCTGGCTCGAGACCCGTACTGTGCCTGGGATGGCATATCCTGCTCCAGG TACTACCCAACAGGTGCACACGCAAAGAGGAGGTTCCGCAGGCAGGACGTTCGGCATGGCAACGCCGCCC AACAGTGCTTTGGACAGCAATTTGTTGGAGACGCGTTGGACAGGACTGAAGAGAGGCTGGCTTATGGCAT AGAGAGCAACAGTACTCTGTTGGAATGCACCCCGCGATCACTACAAGCAAAAGTCATCTGGTTTGTACAG AAGGGACGCGACGTAAGAAAAGAAGAGGTGAAGACGGATGACAGAGTTGTCAAGATGGACTTGGGCTTGC TCTTCCTCAGAGTACGCAAGTCAGATGCAGGGACCTATTTTTGCCAGACAGTAGAACACAATTTTGTCCA TACTGTGCGTAAAATCACCTTGGAGGTGGTCGAAGAGCATAAAGTGGAGGGCATGTTTCATAAGGACCAT GAAGAGGAAAGACATCACAAGATGCCCTGCCCTCCCTTAAGCGGTATGTCTCAGGGGACAAAACCGTGGT ACAAGGAATTCTTGCAGCTGATTGGCTACAGCAACTTCCAGAGAGTGGAAGAATACTGCGAAAAGGTGTG GTGTACAGATAAGAAGAGGAAAAAGCTTAAAATGTCTCCCTCCAAGTGGAAGTATGCCAACCCCCAGGAA AAGAGGCTTCGCTCTAAAGCTGAGCACTTCCGCCTGCCCAGGCACACGCTGCTCTCCTGAGGGCGCCCTC TGCCGGCGGCTGAGGAACCTAGGATGGAAACATTTTTTTAAAGGGGGTTAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_011348
Insert Size 2328 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC057956, AAH57956
RefSeq Size 2935 bp
RefSeq ORF 2328 bp
Locus ID 20349
UniProt ID P70275
Cytogenetics 5 A1
Summary Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011348" in other vectors (4)
SKU Description Size Price
MG210592 Sema3e (tGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e) 10 ug
$910.00
MR210592 Sema3e (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e) 10 ug
$589.00 $710.00
MR210592L3 Lenti ORF clone of Sema3e (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e) 10 ug
$1,010.00
MR210592L4 Lenti ORF clone of Sema3e (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e) 10 ug
$1,010.00

file-alt Citations

New functions of Semaphorin 3E and its receptor PlexinD1 during developing and adult hippocampal formation
Mata, Gil, Pérez-Clausell et al
Sci Rep (2018) 8 (1), 1381 DOI: 10.1038/s41598-018-19794-0
AI Summary: OriGene's full-length cDNA clones for Sema3A (MC205153), Sema3E (MC203244), and Sema3F (MC200862) were used in experiments to investigate the roles of these semaphorins in hippocampal development and function.
Snippet: n addition, some experiments were conducted using commercial full-length cDNA clones Sema3A (MC205153), Sema3E (MC203244) and Sema3F (MC200862) purchased from OriGene Technologies (Rockville, MD, USA). …
Showing 1-1 of 1 papers.
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