Eif2ak1 (NM_013557) Mouse Untagged Clone

SKU MC202874

Eif2ak1 (untagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Eif2ak1
• Synonyms: HCR; Hri
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028923 sequence for NM_013557; GGGGAGGCTCTAGTGGCCGGCAGCGTCTGGGGACCGGCAGAGCGAGGTTACTATCCACGCTCCGAACGGC AATGCTGGGGGGCAGCTCCGTGGACGGCGAGCGCGACACGGACGACGACGCGGCGGGGGCGGTGGCCGCG CCTCCTGCCATCGACTTCCCCGCAGAGGTGTCGGACCCCAAGTATGATGAGTCCGATGTCCCCGCAGAGC TCCAAGTGTTAAAAGAGCCCCTACAGCAGCCCACGTTCCCTTTCCTGGTGGCAAACCAGCTGCTGCTCGT CTCCTTACTGGAACACTTGAGCCATGTGCACGAGCCAAACCCGCTCCACTCCAAACAGGTGTTTAAATTA CTGTGCCAGACTTTTATCAAGATGGGGCTGCTCTCTTCCTTTACCTGCAGTGATGAGTTTAGCTCTCTGA GACTCCACCACAACAGAGCCATCACTCATTTAATGAGGTCTGCCAAAGAGAGAGTCCGTCAGGATCCTTG TCAAGATAATTCTTACATGCAGAAAATCAGATCCAGAGAGATAGCCTTCGAAGCACAAACGTCACGCTAC TTAAATGAATTTGAAGAGCTTGCCATCTTAGGGAAAGGAGGATATGGAAGAGTTTACAAGGTCCGGAACA AATTAGATGGTCAGCATTATGCAATTAAGAAAATCCTGATTAAGAGCGCAACTAAAACAGATTGTATGAA GGTGCTACGGGAAGTGAAGGTTCTGGCAGGTCTCCAGCATCCCAATATTGTTGGCTACCACACTGCGTGG ATAGAACATGTTCATGTGGTTCAGCCACAAGACAGAGTTCCGATTCAACTGCCCTCTCTCGAAGTGCTGT CGGAGCAGGAAGGGGACAGAGACCAAGGTGGTGTTAAAGATAATGAAAGCAGTTCGTCCATTGTCTTTGC TGAACTCACCCCAGAAAAAGAAAAACCTTTTGGGGAGTCTGAGGTTAAAAATGAGAATAACAACCTGGTG AGCTACACGGCCAACTTAGTGGTCAGGAACAGCAGTGAAAGTGAATCGTCCATTGAGCTCCAAGAAGACG GCTTGACTGATTTGTCCGTCAGACCAGTTGTCAGGCATCAGCTGCCGCTGGGGCATAGCTCGGAATTGGA AGGGAATTTTACATCCACGGATGAGTCTTCTGAAGGCAACTTGAACCTGCTGGGGCAGACGGAGGTTCGG TACCACCTGATGTTGCACATCCAGATGCAGCTGTGTGAGCTCTCCCTGTGGGACTGGATAACTGAGAGGA ACAAGCGGAGCCGGGAGTATGTGGACGAAGCTGCTTGTCCCTATGTTATGGCCAGTGTTGCAACAAAAAT TTTTCAAGAATTGGTGGAAGGTGTCTTTTACATACATAACATGGGCATTGTCCACAGAGATCTGAAGCCT AGAAATATTTTTCTTCATGGCCCTGATCAGCAAGTAAAAATAGGAGACTTTGGTCTGGCCTGTGCAGACA TCATCCAGAATGCAGACTGGACCAACAGAAATGGGAAAGGAACACGGACACACACATCCAGAGTGGGGAC TTGTCTCTACGCGTCACCGGAACAGCTGGAGGGATCCCAGTACGATGCCAAGTCAGATATGTATAGCTTG GGTGTGATCCTGCTCGAGCTCTTTCAGCCATTCGGGACAGAAATGGAGAGGGCAACAGTCTTAACAGGCG TAAGGACTGGTCGGATACCCGAATCCCTCAGTAAAAGGTGTCCGGTGCAAGCCAAGTATATCCAGCTCCT AACCGGGAGGAATGCGTCACAGAGACCATCTGCCCTTCAGCTGCTGCAGAGTGAGCTTTTTCAAACAACT GGAAATGTTAATCTCACATTGCAGATGAAGATAATAGAACAAGAGAAGGAAATTGAAGAACTAAAGAAGC AACTAAGCCTCCTTTCTCAGGACAGAGGGCTGAAGAGATGAGAGGGAAAATGGGGCCTACCCTGTGGCCT GAGTTATGCCGCCAAGCATGTGAAAGTGGACTTGCATTACAGTCAATAGGAATGTACATTTCCAACCCTT AGTGAGGACAGGTGTGACTCCCAGTTGTACTGAGAAAACCTATGTAAAGACTTAAAAGGACCCCAGCTTC CTTTTCCTCAACCCTTCCATGACCCAAGACTGGACTAAATGAAGCCTCTTCCTCCCACCTCCTCCAAGTT TTGATTCCTCAGTTTCCTACTCAAATGTTACTCCCCACCCCTGAACCTATTTTTTTAAGCTCTGCCTATT GACCAGGAATCCTGCCTTCCTTCCCGAGGGCTGTACTACAGAATCAGTGCTCCCTCCCCACACCCGCTTG CCTGCCCCAGGATGACTGCCAGCCTGGCTGCTGTTGCTGTGGCAGCTGCAAGTCTTCCCTGGTCCTTCTG TACTGCTGCCATGGCCAGTTACCAGGACACTTGATCACTCCTTGGCTCAAAATGTCTGTGGGCTCTGTAC TGCCTGGAAGGTCAGAGTTCTGCTTCGAAGCACTCTTCCAGCCCTCTGTTCTTTCCACTTGGCATCATCC TGCAGCTTGCCCTAAAGTGATCTCAGCAGCTAAAATGTCTTCTACCTTTATTCTGTATAGTCTCCCATGA TCCGTTGCCTCCTTAAAGCCCCAACTCCCCAAACCACCTGAAGTCCTCAGATAGCAATCTCTGACGCCAT AAAGTGCCTGCCTCTGGCTGGCAGCCATCTCAGTGCACTGCACTTGGTTTTACTCACTTTAAAATAAAAT AAACTCCATAGATGAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_013557
• Insert Size: 1860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC028923, AAH28923
• RefSeq Size: 2761 bp
• RefSeq ORF: 1860 bp
• Locus ID: 15467
• UniProt ID: Q9Z2R9
• Cytogenetics: 5 G2
• Summary: Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions.[UniProtKB/Swiss-Prot Function]