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Cd200 (NM_010818) Mouse Untagged Clone

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SKU
MC202690
Cd200 (untagged) - Mouse CD200 antigen (Cd200), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $450.00
5 Days*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Cd200
Synonyms Mox2; OX2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC054759 sequence for NM_010818
GGGGGCTCTCTCTACGGCGAATAGTAGTGTCCCTGCTCACAGGTGTTGCGGAGATATCCTCCATCGTGGA AGAGCTCAGACCCCGAGAAGCTGGTGTCTAGCTGCGGCCCAGAGCAAGGATGGGCAGTCTGGTATTCAGG AGACCTTTCTGCCATCTCTCCACCTACAGCCTGATTTGGGGCATGGCAGCAGTAGCGCTGAGCACAGCTC AAGTGGAAGTGGTGACCCAGGATGAAAGAAAGGCGCTGCACACAACTGCATCCTTACGATGTTCTCTAAA AACATCCCAGGAACCCTTGATTGTGACATGGCAGAAAAAGAAAGCCGTGAGCCCAGAAAACATGGTCACC TACAGCAAAACCCATGGGGTTGTAATCCAGCCTGCCTACAAAGACAGGATAAATGTCACAGAGCTGGGAC TCTGGAACTCAAGCATCACCTTCTGGAACACAACATTGGAAGATGAGGGCTGCTACATGTGTCTCTTCAA CACGTTTGGTTCTCAGAAGGTCTCAGGAACAGCTTGCCTTACCCTCTATGTACAGCCCATAGTACACCTT CACTACAACTATTTTGAAGACCACCTAAACATCACTTGCTCTGCGACTGCCCGTCCAGCCCCTGCCATCT CCTGGAAGGGTACTGGGACAGGAATTGAGAATAGTACCGAGAGTCACTTCCATTCAAATGGGACTACATC TGTCACCAGCATCCTCCGGGTCAAAGACCCCAAAACTCAAGTTGGGAAGGAAGTGATCTGCCAGGTTTTA TACCTGGGGAATGTGATTGACTACAAGCAGAGTCTGGACAAAGGATTTTGGTTTTCAGTTCCACTGTTGC TAAGCATTGTTTCTCTGGTAATTCTTCTGATCTTGATCTCCATCTTACTATACTGGAAACGTCACCGAAA TCAGGAGCGGGGTGAATCATCACAGGGGATGCAAAGAATGAAATAAGAGCTCTAAAGAAATTATACAGAA CCCTGAACGTGTTTCCCTGGTCTACTTGAATCTGATGTGAAAGAAAAGCAGGAGGGAAAAGGCCATTCTC CATAGGACCTAAGGAGAGCAAAAGACCAGACACGAGCCTGTGAGGGATTTGACTTTTTGCTGTTGTCCCA GGTCCTCGGTGTTTGCATTCCAAGAGGAAGTCGAGTGCCTCGGGTCTGTTGTAGGACTTGATTTTTTTTT TTTTTGTAGAGCAATGCAGTGCCATGCTGTTAGAAAGGCTCCAGACTTAGAACCACCAGTGCCAAGCCAG CTCTCAGACCGACTAGGGCTCCCATCGGAGGAACAAATCGTAGTCAACTTACCTCACAGAGCTCTCTGGT CCTTACACAAAGTAGAAAGGAGTGGGACCAGAAAATTGGCCATGTCTGAAATCTGATGGAATTTTTAGGA AGAAAACTGAAGAATAAGCAAAAGAAGAAAGAACACAGAAGGGTCCAAAGAGCTTCTGAGAGTACCTTTT GCCTTTCTGTTGGTGTCCCAGCTCTGGTTTTGTTCTTAGGTCCGCCAGTGTGTTTCCCTGTTGTTTGAGT ATCTAGTTGACTACCTGCTACTGTTCTGCTGATGGTTGGCCTTGCTAGAATCCCTGACTCCCCTGCCGTT CTCTATGTGCTTCTATGAGGGTTACTATGATGAAAATAGAGCAGAAGATAGTGTGAAGTAACATTGGCAA CTGTAATGTGTCCATTTAACTTATTTTTATAGCACTTAGGCAATATTGTTAGTCTTAGTGAGTAGTTCAC ATCTTTACAAAAGCATGCTCTCCCTATCCATTGGGCCCACAATAACACTCTCTTTGAGGCCATTCTGAAT CCTGTCTCGTGTAATGATAATATATTATGAAAACAGATACTTTAAGAATTTCCTGTACAGCAGTCAGTTG TTTATTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCTCCCCCACCCCAGCTTCTTTTTCTGTGACTTTGTT TTTCATAAAGAGAAGGCATCTCCTGAATACAATCGCTTTGTTCTGAAGACATCGTGAACTATTAATTCTT AACCCTTTGACAAAACTAGTGAAGTTGTTTTCTGTATCTTTTGCTTCATCTGTCTTTATAGAGTGACCTA GGAATTCAAGTGTAAGTTGTTTCCATTGTTGAACTGGATATTTATATACTTGGTATGCTTTTCACGTGTT ATTTAATTCTGTATAATTTCCTATATTTGTATTAAAATATTGAGCAATTAAAAGTGTCAACTAAATATTT GATGTGGCATTCCCTTGAGAAATATAGAAATAAAGAATAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI |
ACCN NM_010818
Insert Size 837 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC054759, AAH54759
RefSeq Size 2296 bp
RefSeq ORF 837 bp
Locus ID 17470
Cytogenetics 16 29.53 cM
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Documents
Resources
"NM_010818" in other vectors (4)
SKU Description Size Price
MG203755 Cd200 (tGFP-tagged) - Mouse Cd200 antigen (Cd200) 10 ug
$489.00 $500.00
MR203755 Cd200 (Myc-DDK-tagged) - Mouse CD200 antigen (Cd200) 10 ug
$289.00 $300.00
MR203755L3 Lenti ORF clone of Cd200 (Myc-DDK-tagged) - Mouse CD200 antigen (Cd200) 10 ug
$600.00
MR203755L4 Lenti ORF clone of Cd200 (mGFP-tagged) - Mouse CD200 antigen (Cd200) 10 ug
$600.00

file-alt Citations

Forced expression of CD200 improves the differentiation capability and immunoregulatory functions of mesenchymal stromal cells
Kim, Kim, Kwon et al
Biotechnol. Lett. (2018)
AI Summary: OriGene’s CD200 Human cDNA ORF Clone was used as the source of the human CD200 gene for forced expression experiments, enabling the study of its effects on mesenchymal stromal cell differentiation and immunoregulatory functions.
Snippet: The coding region of human CD200 from the CD200 Human cDNA ORF Clone (Origene, Rockville, MD, USA) was amplified by polymerase chain reaction (PCR) (Table 1) and cloned into the pEGFP-C1 expression vector (Takara Bio, Shiga, Japan)
Showing 1-1 of 1 papers.
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