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Phc1 (NM_007905) Mouse Untagged Clone

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SKU
MC202540
Phc1 (untagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 1, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $880.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Phc1
Synonyms AW557034; Edr; Edr1; Mph1; Rae-28; rae28
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC052394 sequence for NM_007905
GTTTAGGGTGGCCTTTGACTCTTAGTTTTAATTTTTGATTGAGTTTGCCACCTAAACGGTGTGCTCTCTT CACTTTCTATACTGAATCTGGAACTCACAACATTCATTGAAAAATGAGGATTTTAAGATAGATTTGTGTG CCTGCGAGGTTTTTGATGGCAGTTTCCCTGGTGAAAAGACTTTAAAATTGGCCCTCTCTGTTTCATTATC TGGGGGAAATGAGGGGGTCATCGAATTGATGAACGTGTTTTATATAAGGCTCTATTGCAAGTAACAGGGT CTTACGTCTCTGAGTGCAGTGAGATAACTCCAGTAATCTGTACAACTGTGCCATGCTGCCTCTAATCCTT ACCCACTTTTTTTCCTTCACATGCTGATATTGAGGATTCATGGGGCTTGAGTCAGAGCGCCATTCTTGGG GACCCTGGAACGCTATCATGGAAACGGAGAGTGAGCAGAACTCTAGTTCGACCAATGGGAGTTCCAGTTC AGGGGCCAGCTCTCGGCCCCAGATAGCACAGATGTCCCTGTATGAACGGCAAGCAGTGCAGGCTCTGCAA GCACTGCAGCGGCAACCTAATGCGGCTCAATATTTCCACCAGTTCATGCTCCAGCAACAGCTCAGCAATG CCCAACTGCACAGCCTGGCAGCTGTCCAGCAGGCCACGATTGCTGCCAGTCGACAGGCTAGCTCCCCAAA CAGCAGCACTGCACAGCAGCAGACCGCTACCACCCAGGCCTCGATGAATCTCGCGACCACGTCAGCTGCT CAGCTCATCAGCCGATCGCAGAGTGTGAGCTCCCCCAGTGCTACCACCCTGACGCAGTCGGTGCTGCTGG GGAACACCACTTCCCCACCTCTCAACCAGTCCCAGGCCCAGATGTATCTGCGGCCACAGCTGGGCAACCT ATTGCAGGTTAACCGGACCTTGGGCCGGAATGTGCCTCTTGCCTCCCAGCTCATCCTGATGCCTAATGGG GCAGTGGCTGCAGTCCAGCAGGAGGTGCCACCCGCTCAGTCTCCTGGAGTTCATGCAGATGCAGATCAGG TGCAGAACTTGGCAGTGAGGAACCAACAGGCTTCAGCTCAAGGACCCCAAATGCCAGGCTCCACTCAGAA GGCCATCCCTCCTGGAGCTTCTCCTGTTTCTGGCCTCTCCCAGACTTCTAGCCAGGCCCTAGCCGTGGCA CAGGCTTCTTCTGGGGCCTCAGGCCAGTCACTCAACCTTAGTCAAGCCGGTGGAGGCAGTGGGAATAGCC TCCCAGGCTCCATGGGTCCAGGTGGAGGTGGCCAGGCGCCTGGGGGTTTAGGTCAGTTGCCTTCTTCAGG ATTGACTGGTGGAAGCTGTCCCCGGAAGGGCACAGGAGTGGTGCAGCCCTTACCTGCAGCTCAAACAGTG ACTGTGAGCCAAGGAAGCCAAACAGAAGCAGAAAGTGCAGCAGCCAAGAAGGCAGAAGCAGATGGGAGTG GCCAGCAGAGTGTGGGCATGAACCTGACTCGGACAGCCACACCTGCCCCCAGCCAGACGCTTATTAGCTC AGCCACATACACACAGATCCAGCCCCATTCACTGATTCAGCAACAGCAGCAGATCCACCTCCAGCAGAAG CAAGTGGTGATCCAGCAGCAGATTGCCATCCACCACCAGCAACAGTTCCAACACCGCCAGTCCCAGCTGC TTCACACAGCCACACACCTCCAGTTGGCCCAGCAGCAGCAGCAGCAACAGCAGCAACAGCAGCAACAACA GCAACAGCAGCAGCAGCAGCAGCAAGGAACAACCCTCACTGCCCCTCAGCCACCCCAGGTCCCACCTACT CAGCAGGTCCCACCTTCCCAATCACAACAGCAAGCCCAGACTCTGGTGGTCCAACCCATGCTTCAGTCTT CACCCCTGACTCTTCCACCTGAACCAACCTCTAAACCACCCATCCCCATTCAGTCCAAACCTCCTGTAGC GCCTATCAAACCTCCTCAACTAGGAGCTGCTAAGATGTCAGCTACACAGCAGCCACCACCACATATCCCC GTGCAAGTTGTCGGTACCCGGCAGCCAGGTTCAGCCCAAGCACAGGCTTTGGGATTAGCACAGCTGGCTG CTGCTGTACCTACTCCCCGTGGAATAACAGGAGCAGTCCAGCCTGGCCAGGCTCATCTGGCCTCCTCGCC ACCTTCATCACAGGCGGCTCCTGGTGCACTTCAAGAATGTCCTCCTGCATTGGCTGCTGGGATGACCCTT GCTCCTGTGCAGGGGACAGCACATGTGGTAAAGGGTGGGCCTACAGCCTCCTCTCCTGTTGTGGCCCAGG TCCCTGCTGCTTTCTACATGCAGTCGGTGCACCTGCCGGGTAAAGCCCAGACCTTGGCTGTGAAACGCAA AGCTGAGTCTGAGGAGGAGAGAGATGACCTCTCCGCATTGGCCTCTGTGCTTCCTACAAAGGCCTCCCCA GCAGCAGAGAGCCCAAAGGTAATAGAGGAGAAGAATAGTCTTGGAGAGAAAGCTGAACCAGTGGCCAGTT TGAATGCTAATCCTCCAAACAGTGATCTAGTAGCCTTGGCCCCTACCCCATCAGCACCACCTCCTACTCT GGCCTTGGTGTCCAGACAGATGGGTGACTCAAAACCCCCACAGGCCATTGTGAAGCCCCAGATTCTCACG CACATCATTGAAGGCTTTGTTATCCAGGAAGGAGCAGAGCCTTTTCCGGTGGGATGTTCTCAGTTCCTGA AAGAAACCAAGAAGCCACTGCAGGCTGGCCTTCCGACCGGGCTGAATGAGAGTCAGCCAAGTGGCCCTTT GGGGGGAGACAGCCCCTCTGTAGAGTTAGAGAAGAAGGCAAATCTCCTCAAGTGTGAGTACTGTGGCAAG TACGCCCCAGCAGAGCAGTTTCGAGGCTCTAAGAGGTTCTGCTCCATGACGTGTGCAAAGAGGTACAATG TGAGCTGCAGCCACCAGTTCCGCTTGAAGAGGAAAAAAATGAAAGAGTTTCAGGAAGCCAGCTATGCACG TGTTCGGAGGCGTGGACCCCGCCGCAGCTCCTCTGATATTGCTCGTGCTAAGATCCAGGGCAAGCGCCAT CGGGGTCAAGAGGACTCTAGCCGGGGATCAGATAATTCCAGCTATGATGAAGCACTCTCTCCAACATCTC CTGGGCCACTGTCAGTAAGAGCCGGGCACGGAGAACGTGATCTAGGAAACACCATTACAACCCCATCCAC GCCAGAGTTACAGGGCATCAATCCCGTGTTCTTGTCCAGTAATCCTAGCCAATGGAGCGTCGAGGAGGTC TACGAGTTTATTGCTTCTCTGCAAGGCTGCCAAGAGATTGCAGAAGAGTTTCGTTCCCAGGAGATTGATG GACAGGCCCTTTTATTACTTAAAGAAGAACATCTGATGAGTGCCATGAACATCAAATTAGGTCCTGCTCT CAAGATCTGTGCCAAGATCAATGTCCTCAAGGAGACCTAAGTGACCCTCTGCACAATTCAGACTAAGGCA GAAGGCCTGGCTGTTCAGGTTATAACCTGGAAGAGCTGTTCCTTTTGTGTGACCTGTGGGGTTTGGAGGC AGAACTCTAAGGATTGGTTGCTGGTGCTACATGGTGACAGCTTTGATACCTTCTCTGGGTTTCACTTTAA CAATAAAAACCTTCTCACTATTCACACCTACCCTAATCCAGTTTTTAAACAGGCAGACTAGAGACTAGGA CTGCTTAGCCTTCCTTATCCTGGAGTGGAGGGACTAGCCAGGGTTCACTTCTCCAAGGAGGAATGGATGG TAAGGCAAGGAAATGGATGCCATGTAGGTTTGCCTCCCTATGGGAGAGGTCAGCTTTCCTAGCTTATGAC AGGAGCAGCAAACCTGGTACTCTGCTGGAGTCTCACTGTGGCTGTAGCTCAGTTTTCTGTTTCATTTTCT CCCTTAATACTGAATATTTCTTCTGCATTCATGGCAGGGTCACCAGCCATCATAGACACTTGGTTGGTAT CGGTTTTGCTGCAGGGACGAAGTGTTTGAGTGACACATGTGGCTGTTGCCATTCTTTCTGCACCCCAGCT CCCAACTGTTCCCACCTTCAACCCTCTTTCCCATTCCAACGCTGCTCTGTCCTATAGCTTCACAAAACAG GAGCGTGTGGGGCTGAGGTCAGGACTGTACCTTGGGCACTATTCCTTATACAAAATATTAAATATTTTTT TTCCTCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI |
ACCN NM_007905
Insert Size 3033 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC052394, AAH52394
RefSeq Size 4237 bp
RefSeq ORF 3033 bp
Locus ID 13619
Cytogenetics 6 57.52 cM
Summary Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Required for proper control of cellular levels of GMNN expression (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the predominant isoform (a). Both variants 1 and 3 encode the same isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_007905" in other vectors (4)
SKU Description Size Price
MG211438 Phc1 (tGFP-tagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 1 10 ug
$1,520.00
MR211438 Phc1 (Myc-DDK-tagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 1 10 ug
$1,320.00
MR211438L3 Lenti ORF clone of Phc1 (Myc-DDK-tagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 1 10 ug
$1,620.00
MR211438L4 Lenti ORF clone of Phc1 (mGFP-tagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 1 10 ug
$1,620.00

file-alt Citations

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