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Slc7a8 (NM_016972) Mouse Untagged Clone

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SKU
MC202413
Slc7a8 (untagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 (Slc7a8), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $495.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Slc7a8
Synonyms AA408822; LAT2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC059004 sequence for NM_016972
CGGAGCTCGGAGCCGACAAGGGCGAGAAGGAAGGGGCGACCGCAGCAGGTGGAAACCTGATCCAGAGCCG GCGAGCAAGAAAGTACCTGAGCACGGCTCCTCCTCCGCTCCCTCAGCTAAGAAGAACTACTTTTACCCTC ATCGTAGAAGAGACTTAAGGGAAGGTGGAGGCGATCTGTTTCATTAGGAGGGCTGCAGGGAAATCCTGAC CAGAGCAAGCCGCCGCCCGGTACACCGAGCAGAGCGATCCTGGGCTTCTGAAGATAAACGCTTTCCGGAG AATTCCAGCTGGAGTCGCTGGTACAGTTCTATTTTTGTATTTATATATATATATATTCTTTGCCTTCCAT TTAAAAACACGGCCCTTGTTTGTTAGGCAGTGACACGTGTGACTCTCATTTGCTGTCACTTTTAGAGGGT AGGAGCGCACTCTGTTTTCGGAAAAGGGCGTTCTAATTGGAAACTCTGTTTTTCCACATATCTATACGTT TACACGCACGAGCGTTTAGAAAAAGACTGCTCTTTTTCGCTTTGGGTGGTCGGAACCCTACGGAGAAAGA TGGAAAAGGGAGCCCGCCAGCGAAACAACACCGCGAAGAACCACCCGGGTTCTGACACCAGCCCTGAGGC CGAGGCTAGCTCGGGAGGGGGCGGAGTAGCCCTGAAGAAAGAGATCGGATTGGTCAGCGCCTGTGGTATC ATTGTAGGGAACATCATTGGCTCCGGAATCTTTGTCTCACCGAAAGGTGTGCTGGAAAACGCGGGCTCTG TGGGCCTTGCTCTCATTGTCTGGATCGTGACAGGCATCATCACAGCCGTGGGAGCTCTCTGCTATGCTGA GCTAGGCGTCACCATCCCTAAGTCTGGAGGTGATTACTCTTATGTGAAGGACATCTTCGGAGGACTGGCT GGGTTCCTGCGGCTGTGGATTGCTGTGCTGGTGATCTACCCCACCAACCAAGCTGTCATCGCCCTCACCT TCTCCAACTACGTGCTGCAGCCGCTCTTCCCTACCTGCTTCCCCCCTGAGTCCGGTCTGCGACTCCTGGC TGCCATCTGTTTGTTGCTCCTCACATGGGTCAACTGCTCCAGTGTCCGATGGGCCACCCGGGTTCAAGAT ATCTTCACAGCTGGGAAGCTCCTGGCCCTGGCTCTGATCATCATCATGGGTATTGTGCAGATATGCAAAG GAGAATTCTTCTGGCTGGAGCCAAAGAATGCATTTGAGAATTTCCAAGAACCTGACATCGGCCTCGTTGC TCTGGCATTCCTCCAGGGCTCCTTTGCCTATGGTGGCTGGAACTTCCTTAATTATGTGACTGAGGAACTT GTGGATCCTTACAAGAACCTTCCTAGAGCCATCTTCATCTCCATCCCACTGGTCACATTTGTGTACGTCT TTGCTAATATTGCATACGTCACTGCAATGTCCCCCCAGGAGCTGCTAGCCTCCAATGCAGTTGCTGTGAC TTTTGGAGAGAAACTCCTCGGGGTCATGGCCTGGATCATGCCCATTTCTGTTGCCCTGTCCACGTTTGGT GGAGTCAATGGCTCCCTCTTCACCTCCTCCCGGCTGTTCTTTGCTGGAGCCAGAGAAGGCCACCTTCCCA GTGTGTTGGCCATGATCCACGTGAAGCGCTGCACTCCAATCCCAGCCCTGCTCTTCACATGCCTCTCCAC CCTGCTGATGCTGGTCACCAGTGACATGTACACACTCATCAACTATGTGGGCTTCATCAACTACCTCTTC TATGGGGTAACGGTTGCAGGACAGATAGTCCTTCGCTGGAAGAAGCCTGACATTCCCCGCCCCATCAAGG TCAGCCTGCTGTTTCCCATTATCTACCTGCTGTTCTGGGCCTTCCTGCTGATCTTCAGCCTGTGGTCAGA GCCAGTGGTGTGTGGCATTGGCCTGGCCATTATGCTGACAGGAGTTCCTGTCTACTTCCTGGGTGTCTAC TGGCAACACAAACCCAAGTGTTTCAATGACTTCATTAAGTCCCTAACCCTAGTGAGTCAGAAGATGTGTG TGGTCGTGTATCCCCAGGAGGGGAACTCGGGGGCTGAGGAAACAACTGATGACTTAGAGGAGCAACACAA GCCCATCTTCAAGCCTACTCCTGTCAAGGACCCGGATTCGGAGGAGCAGCCCTGAAGACTGCCAGCCTGT AACTGGCCACTCCTCCCTTCATCCTTTCTGCCCTGTATTCCTGCCTAGGTCCCCCCAACACACACACACA CACACACACACACACACACACACACACACACTTCTGTAAGGCAGGGGCCAGACCTGGGTGTCCACAGTGA GACATTCTAAACAAAGACCCTGACCTTTGTACCCAAAGAACCAACCTGCTTCCAGGACCGAGGCCCATGG TCAAGGTCAATGCACCAGGGCCTGCACACACCCTAAGGTTTGGAGGAGAGTGGAGGTGCCATTAGTACCC TACAGAGCCCTTCCTCCTGGGCCCATGCCTGTTAGGTGCCTCTAAGAAACCTGGGTTCACTACTATTTCT TCTCCCTAACCCTGAGCCCAGGCGAAACTTCCATTGGGGACAAAAGGTGCAGCCCCAGCAATAGCTAGGA AGACAACTCAGATTGTCACCGTTAAGTCAGCTGGAGAGACTCAGATGTGGCTTCATTCTCCAGGGAACCA AAAGGCAGAGGGTACACTTCTCTTTGCCTTCTTCTCCACTCTGTACTACCAAGACTCCACTGCCCAGCCT CAACCCACTACCCAGAGAGTTCCCTTTCAGGAAGCCTCCACCCCACTGAGCTAAGGACCAAGAAGGAGGA CTGCCCCACCCCCAGCTCTAAGCCAAGCTTGTGGAATATACAGGGAATGCTCAGACTGCAGAAGCCAAGC CCTGCCCCACCCCTGCCGCTGCCTCCTATCCCCTTGTGGTGCCAAAGCTCCCTGAAGCCGGAAAGGCTTC TATTTAGTCTAATAGACATCTCTCTCTTAAGGGCAAAGTGACTCTGCCCCTCCCTGTCCCCCTGTGAAAG AGCAGTCACCCTCTTATCCCAGCAGCTCTTCCCACCCACAGCTCTGCCTACCACCCTCACCCCTTTCCTC TCCTGGACCACGGCTCAAGGACCAGGTATGAAGGATCTCCAAGTCCTTCAGGCCTGAATTAGCATCAAAC CAGCCTTAAGTCATCTCCCATCCAAGAACTGGGCCTAAAGTGCTCCCAGTTTCCAACCTCCAGGACAGAC CTGATATTAAGTCCTTCCCTGGAAGGAAGGGTGCTTCCCCCTTCCCAGCAGTGCTCAGCCCTCACCCTGG GGAGACCATGTTGTTGGGGGGCACGGGGGAGTGAGGAGAATGTTTTCCGAAGGCCTGTTCTTCTCCCCTC CCCCAACTCAAACAACCCTACCACCGTTCTGCCATCCCACCTGGGACAAGAGGACAGAATGGCACTGACT TTGGAGACCTTAGAAAAACCCCTTCCAGGAGAGCTTCTGGCCTGTGCTCTACAGGAAGGTGACAGTGTCT GCCTTCTCCTGGCAGCCTCCACACCTGCTGTGCTTAGGACAGGATCCTTTGATCACAAGGCAGAACCCTA ACCCCGGTCCATCTACTCAAACTGGAATATGCAGGGACCTTTCCCTGGCCCCTGCCAGCAGGTCTCCCAA CTTCCTACCTAGCTCACCCTACCCCTAACTGGTGAGAGGCTGAGTTTGGGCTGAATTATATGCCCTTAGG AATACAGTCCAGGCAGGCTGTGGGGCCTCTGGGGGGCCAGGGACCACCCGCTACCCCTCATTCCTCTCAT TCCTTCAGCCTGTACCTGCCCCTCCTTGAATTATTGTCTCTCTCTCTCTCTCTTTTTTTTTAAGTGGATG CCTTACTTTTTGGATAACTATTTTTGAAGCTGGTATTTCTATTTCTTTTGGATTTTTTAATGTATGGCGG TTTGGGGGCAGAGCTAGAACCTTAATAATCTGTCACTGAGTTCATTTAGGTTTTAAATTTTATTGGTTTG TTTTATGGAGTATTTTCTTTTCATGTAATAAAATTTTAAATGGAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Restriction Sites AscI-NotI |
ACCN NM_016972
Insert Size 1596 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC059004, AAH59004
RefSeq Size 4064 bp
RefSeq ORF 1596 bp
Locus ID 50934
UniProt ID Q9QXW9
Cytogenetics 14 C2
Summary Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.UniProtKB/Swiss-Prot Function
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Resources
"NM_016972" in other vectors (4)
SKU Description Size Price
MG208519 Slc7a8 (tGFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 (Slc7a8) 10 ug
$695.00
MR208519 Slc7a8 (Myc-DDK-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 (Slc7a8) 10 ug
$289.00 $495.00
MR208519L3 Lenti ORF clone of Slc7a8 (Myc-DDK-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 (Slc7a8) 10 ug
$795.00
MR208519L4 Lenti ORF clone of Slc7a8 (mGFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 (Slc7a8) 10 ug
$795.00

file-alt Citations

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