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Gnai3 (NM_010306) Mouse Untagged Clone

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SKU
MC202370
Gnai3 (untagged) - Mouse guanine nucleotide binding protein (G protein), alpha inhibiting 3 (Gnai3), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gnai3
Synonyms AI158965; AW537698; Galphai3; Gnai-3
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC041107 sequence for NM_010306
GCGATTCCCGCGGTGTGTGTGAGTGAGCCCGGGCCCGGTCCCCTCTCCCGCCGCCGCCATGGGCTGCACG TTGAGCGCCGAGGACAAGGCGGCGGTGGAGCGGAGCAAGATGATCGACCGCAACTTGCGGGAGGACGGGG AGAAAGCGGCCAAAGAAGTGAAGCTGCTGCTGCTCGGCGCTGGAGAATCTGGTAAAAGTACCATCGTGAA ACAGATGAAAATCATTCATGAGGACGGCTATTCAGAGGACGAATGTAAACAGTATAAAGTAGTTGTCTAC AGCAATACTATTCAGTCCATCATTGCAATCATACGAGCCATGGGACGGTTGAAGATTGATTTTGGGGAAT CTGCCAGAGCAGATGATGCCCGACAGTTATTTGTTTTAGCTGGGAGTGCTGAAGAAGGAGTCATGACTTC AGAACTAGCAGGCGTGATTAAACGTTTATGGCGAGATGGCGGGGTACAGGCATGCTTTAGCAGGTCCAGG GAATATCAGCTCAATGATTCTGCTTCATACTACCTAAATGATTTGGATAGAATATCCCAGACCAACTACA TTCCAACTCAGCAAGATGTTCTTCGGACAAGAGTGAAGACTACAGGCATTGTGGAGACCCACTTCACCTT CAAGGAACTCTACTTCAAAATGTTTGATGTAGGTGGCCAAAGATCCGAACGAAAAAAGTGGATTCACTGT TTTGAGGGAGTGACAGCAATTATCTTTTGTGTGGCTCTCAGTGATTACGACCTTGTTCTGGCTGAGGATG AGGAAATGAACCGAATGCATGAAAGCATGAAATTGTTTGACAGCATTTGTAACAACAAATGGTTTACAGA CACTTCAATCATTCTCTTTCTTAATAAGAAAGACCTTTTTGAGGAAAAAATAAAGAGGAGTCCATTAACA ATCTGTTATCCAGAATACACAGGTTCCAATACATACGAAGAGGCAGCTGCTTACATTCAGTGCCAGTTTG AAGATCTGAACCGAAGAAAAGATACCAAGGAGGTCTACACTCACTTCACCTGTGCCACAGACACCAAAAA TGTGCAGTTTGTTTTTGATGCTGTTACGGATGTCATCATTAAAAACAACTTAAAGGAATGTGGGCTTTAT TGAGAGGATGGCATAGTAAAAGCTATTACAGGGAGGAGTGTTGAGACCAGATGTCATCTACTGTCTCTTG GGTCAGCAGCACGCATGACAGGACCAAGGAATGGCAGCAACACGCAGAATCTTAGCTAGCGCTCTCCAGC AGTCTTCTCTATTAGGGAATGTCTAATTGGCATGAGATGTTCAAATCAGACCTGGGATTGGACAACTGTA AAGTATGACTGGATCGTCAGGGCGTCGCTTGGACTCTGTAATCTAATGTTTAGGGCATATTGAAGTTGAG GTGCTGCCTTCCAGAACTTAAACATGTAGCTCACTTTCCCCCATCTTTTTGACAAATGACCAGTAGTTAA TTTCTAAGGTTTTTCTTATCAAGAGAAGAATACTTAAAAACTCTTCCTTGTTTGCAAAAGAAACTTCTGA GGACTGAGTCTTAACTATGCACACAATGTCACCAGACATCTTGAAACTATTCCTCTTAGTAGGAACTGTT TGTTTTTAACTCTTGGAATGGTCGGGAGTAATATTTCAATAATTAAAATTCTTCCTGGTTATTGGGACTA TATTAATAGAGCTTTTCGGAATGAAATTTATGTTACTGTCATGTTTAAAGTACCACAATGGTTTCTAAAC AGCATTCACCTTGGAGAGTTCTACAGTACGATTCCTGCTCTCATTGCTTTACGCTTACGGTTGGAAGTTG TATTTGTCGGGGTTAACCTTGTTTGTGCTGATTACCAAATTACTAGATGAATACTGATGTAGTGTGTTAA ACTGCTTCTTGGACACACGCAGGATAGCCGTCAATGAGATGCAGATGCTAAATTGAGAACCAGTGGCTTG CTGCTACAGGTCTACTAAGTTGACCACATGGAATCTTGCCTGTTTGTCTCGGTCATGGGGAGGTTTTGAA TAAGATGCCCCTTGTTTTCCATCTTCGTGGCCTTTTTTGTTAGGAGACATCTAAAAGTGTATTAACAGTG CATGCTTTTCCTTTGTAAATGTGGTGCCAAATCCCTGTTTGCCATCGTTTTTACTGTAGCAATGTTAACT GTAGTAGTAGTCCTTACTATATTCTTTTTCTAAAACTTGCATTTGGGGACTTTTTCCACCTTGTCGTGTA CAGATTTGAATCTGTTACAGTTGGCAGCAGTATTAAAACAAACGGTGAGTGAAGAGCCTACGGTTGCTCC GTGCATAACTAGTTCTTTCTGGGGATCCTGTCTGTCTCTTTTTTCCCCTGACCTCTGTCTGAGAGTGGAT TTATTAGGTAGAAAAGTGTCTTTGAAAGTCTAGCTCCTTGATGTAATAGTATTACCAAGTCAGGACTGAG GGCTTTATGTGCAGTCCGTGTACAAGAGGAAAGTCACAATCCGGCTTTCGGATGTGTACAGTTTGCTTGT GCTGAGAGCTACAGTCCTTTTGTACAGTATATATCAAATGTCATTAAACACTGAGTGGACACTGAGGGAC AGCAAACAATGCATTTGGAGCATTTCAGATCAGTAGTTTGATCAGTGCCTTTTGAGGTCCAGTCGCTGTG GCTCGGATCCATTCATCTCTATGTTACCTCCTACTCGTTTGAAAGAAGGTTGATGGTCAGAACTGCTTCA GAAGCAAGCCACGGTGATCAGTGCCTTTGTTGGCAGCTTTTACAATTTTTAATTCTTACGTGTTTTGCAA AATCATTATTATGCAGACAGTATAACTGTTATCTTACAAATATTTCGTTAGGGCTAGGAAAATGTCACTT GAAAGAAAGAAAAGTATTGATTTCAATCGGCGATTGACTCAATTCCCACAATGTAGGGGCTAATAAGTCT TACTCTGAATTTATCTTTCCTTTGTTGTATTCTGTAATATAAGAACATCTGGTGAAATATTGTTTTCATA ATTTTTCTATACTTTGGGTTTAATAAAATGGTGTTGATAATCTAAAATATTAGATAATCCAAATTTAATT TTACTTCAGAATGCATTTTGTATTTTCATTTGAATCTGTCTATTTTTTAAACCTTTCTGTTAATTAAAAA TAGAAGTTCTTCACTTCTTTGTCTCTGCAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_010306
Insert Size 1065 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC041107, AAH41107
RefSeq Size 3193 bp
RefSeq ORF 1065 bp
Locus ID 14679
UniProt ID Q9DC51
Cytogenetics 3 46.83 cM
Summary Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. Stimulates the activity of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.UniProtKB/Swiss-Prot Function
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"NM_010306" in other vectors (4)
SKU Description Size Price
MG205391 Gnai3 (tGFP-tagged) - Mouse guanine nucleotide binding protein, alpha inhibiting 3 (Gnai3) 10 ug
$657.00
MR205391 Gnai3 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), alpha inhibiting 3 (Gnai3) 10 ug
$289.00 $457.00
MR205391L3 Lenti ORF clone of Gnai3 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), alpha inhibiting 3 (Gnai3) 10 ug
$757.00
MR205391L4 Lenti ORF clone of Gnai3 (mGFP-tagged) - Mouse guanine nucleotide binding protein (G protein), alpha inhibiting 3 (Gnai3) 10 ug
$757.00

file-alt Citations

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