Acot1 (NM_012006) Mouse Untagged Clone

SKU MC202249

Acot1 (untagged) - Mouse acyl-CoA thioesterase 1 (Acot1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Acot1
• Synonyms: ACH2; CTE-1; CTE-I; Cte1; D12Ucla1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028261 sequence for NM_012006; CCACGCGTCCGGCTCGAGATGGAGGCGACGCTGAACCTTGAGCCCTCGGGCCGCAGCTGCTGGGACGAAC CGCTGAGCATCGCCGTGCGCGGCCTGGCCCCGGAGCAGCCCGTCACGCTGCGCTCTGTCCTGCGCGACGA GAAGGGCGCGCTCTTCCGAGCCCACGCGCGCTACCGCGCGGACTCCCACGGCGAGCTGGACCTGGCGCGC ACGCCCGCGCTGGGCGGCAGCTTCTCGGGGCTCGAGCCCATGGGGCTGCTCTGGGCCATGGAGCCCGATC GGCCTTTTTGGCGATTGGTCAAGCGCGACGTGCAGACGCCCTTCGTGGTGGAGCTGGAGGTGCTGGACGG ACACGAGCCCGACGGCGGGCAGCGGCTTGCACATGCGGTGCACGAGCGTCACTTCTTGGCTCCGGGGGTG CGGCGCGTGCCCGTACGAGAGGGCCGGGTGCGCGCCACGCTCTTCCTGCCCCCAGAACCTGGACCCTTTC CTGGGATCATAGACCTTTTTGGAGTTGGAGGTGGCCTTCTGGAGTATCGGGCGAGCCTGCTGGCTGGGAA GGGCTTTGCCGTCATGGCTCTGGCTTATTACAACTACGATGACCTCCCCAAGAACATGGAAACCATGCAC ATGGAGTACTTTGAAGAAGCCGTGAACTACCTGCGCAGCCACCCCGAGGTAAAAGGACCTGGAATTGGGC TGCTTGGGATTTCCAAAGGGGGTGAACTTGGCCTTGCTATGGCCTCCTTCCTGAAGGGCATCACAGCTGC TGTGGTCATCAATGGCTCCGTGGCTGCTGTTGGGAACACCATCTCCTACAAGGATGAGACTATACCCCCT GTGACTATCCTGAGAAATCAGGTCAAAATGACCAAGGATGGCCTCAAGGATGTTGTAGATGCTTTGCAAA GCCCTCTGGTAGACAAGAAGAGCTTCATTCCCGTGGAAAGGTCTGACACGACCTTCCTGTTCCTCGTAGG TCAGGACGACCACAACTGGAAGAGCGAGTTCTATGCTGATGAGATCTCCAAACGCCTGCAGGCGCACGGG AAGGAGAAGCCCCAGATCATCTGCTACCCAGCAGCAGGGCATTACATCGAGCCCCCTTACTTCCCGCTGT GCAGCGCTGGCATGCACCTCCTGGTGGGTGCCAACATCACCTTTGGAGGGGAGCCCAAGCCTCATGCCAT GGCCCAGTTGGATGCATGGCAGCAGCTCCAGACTTTCTTCCACAAACAGTTGGGTAGTGAGTGTTTGCAT GTGTCTCCTAAAATATAACCTATTATATGATGGTTTGGAGGTTGGGGAAAGGTACAAATACATTGTAAGA ATATCATTTCAGATGACTTCATCTGAACATAAAACTGCTTTAGATTTACATTTAAAAAAATTGATATAAT TATCAAAATAGTGCTGATTCAAGGGCTGGGAATGGAGTTTCATTGTCAAGTCCTTGACTGGCGCATGCAG GATCCTGGGTTCAATCCAGCTGCGAGAAATAAAGCACGTTCTATGGAAACTGGAGCTATCCAAGAAAAGT GCCAGGAATAAAAAGGAACCATGATTAAACACAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_012006
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC028261, AAH28261
• RefSeq Size: 1587 bp
• RefSeq ORF: 1260 bp
• Locus ID: 26897
• UniProt ID: O55137
• Cytogenetics: 12 39.0 cM
• Summary: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating intracellular levels of acyl-CoAs, free fatty acids and CoASH. More active towards saturated and unsaturated long chain fatty acyl-CoAs (C12-C20).[UniProtKB/Swiss-Prot Function]