Tbce (NM_178337) Mouse Untagged Clone

SKU MC202031

Tbce (untagged) - Mouse tubulin-specific chaperone E (Tbce), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Tbce
• Synonyms: 2610206D02Rik; C530005D02Rik; pmn
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC050206 sequence for NM_178337; GCCGAAAGCTTCGTCGCTGATTGCGTGAGGGTCTCTGGGTGCTGACAGACCGGCAGAGGGAAGATCTCCT ATTGTGGATTCCTGCTATCTTATAATGAGTGACATTTTGCCATTAGATGTCATTGGTAGAAGAGTTGAAG TTAATGGAGAATATGCAACAGTGCGCTTTTGTGGTGCTGTTCCTCCTGTGGCAGGACTCTGGTTAGGAGT TGAGTGGGACAATCCCGAGAGAGGAAAGCATGACGGGAGCCACGAAGGTACTATGTATTTTAAATGCAGG CACCCAACAGGAGGCTCTTTTGTTCGTCCAAGCAAAGTTAATTTCGGAGATGACTTCCTTACTGCCCTTA AGAAGCGCTATGTGCTCGAAGATGGACCTGATGATGATGAAAATTCGTGTTCCTTAAAAGTTGGAAGTAA ACAAGTGCAAACTATTGGTTTTGAACATATTACAAAGAAGCAGAGTCAGCTGAGAGCACTGCAGGACATT TCCCTCTGGAACTGTGCCGTGAGCCATGCTGGTGAACAAGGAAGAATTGCTGAAGCCTGCCCCAATATTC GAGTTGTAAATTTATCCAAAAACTTGTTGTCAACTTGGGATGAAGTGGTTCTTATTGCAGAGCAGCTCAA AGACCTGGAGGCTCTTGATCTCAGTGAAAATAAACTGCAGTTTCCCTCTGACTCACCAACTCTAACTAGA ACATTTTCTACTCTGAAGACTTTAGTCCTAAACAAAACAGGAATAACGTGGACTGAGGTGCTGCACTGTG CTCCTTCATGGCCAGTCCTCGAGGAACTTTACCTCAAGTCTAACAATATTTCCATTTCTGAAAGGCCAGT GAATGTCCTGCAGAAAATGAGGTTACTAGACCTTTCCTCCAACCCATCAATTGATGAAAGCCAGCTGAGC CTTATAGCAGATCTTCCAAGATTAGAACACCTAGTCCTTTCTGACATTGGACTTTCTTCTATACATTTTC CAGATGCTGAAATTGGATGCAAAACATCCATGTTCCCGGCCTTGAAGTACCTCATAGTCAATGACAACCA GATATCAGAATGGTCATTTATCAACGAGCTGGATAAACTGCAGAGCCTGCAGGCCCTGTCCTGCACTAGA AACCCTTTGAGCAAAGCGGACAAAGCAGAGGAGATCATTATCGCCAAGATTGCTCAGCTGAGGACCCTGA ACAGGTGCCAGATTCTTCCAGAGGAAAGGCGTGGAGCTGAGCTAGATTATCGAAAAGCCTTTGGAAATGA ATGGAGAAAGGCTGGTGGACACCCAGATCCAGACAAAAACAGGCCAAATGCAGCGTTTCTCTCAGCCCAT CCTAGATACCAGCTTCTCTGCTGCAAATATGGTGCACCTGAAGATGAGGAGCTCAAAACACAGCAGCCAT TCATGCTAAAAAAACAGCTACTAACCCTGAAGATAAAATGCTCAAATCAACCTGAACGGCAAATATTAGA AAAGCAGTTGCCAGACTCCATGACAGTTCAAAAGGTAAAGGGACTGTTGTCACGTCTTCTCAAAGTTCCT GTGTCAGAACTTCTGTTGTCCTACGAAAGTTCCAAGATGCCGGGCAGAGAAATTGAACTAGAAAATGACC TACAGCCATTACAGTTTTATTCTGTGGAAAATGGAGATTGCCTACTAGTGCGATGGTAACAGTCCAACCG TAAGAGTTTAACGACTGGAATGTATACACCAGAAACAAAACCTTTGCCCTCACAAATGATGTTATGTTTT GTTGGGAAAAGACCATTTGGTTGTATATTAATAGAATAATAAAGGCTTTGATTACTAAAAAAAAAAAAAA AAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_178337
• Insert Size: 1575 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC050206, AAH50206
• RefSeq Size: 1827 bp
• RefSeq ORF: 1575 bp
• Locus ID: 70430
• UniProt ID: Q8CIV8
• Cytogenetics: 13 5.29 cM
• Summary: This gene encodes a tubulin binding cofactor that participates in microtubule dynamics. A mouse model of progressive motor neuropathy (pmn) was discovered to harbor a single amino acid deletion in this gene. Mice that are homozygous for pmn allele exhibit progressive atrophy and premature death due to respiratory failure. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2015]; Transcript Variant: This variant (1) encodes the functional protein.