Gulo (NM_178747) Mouse Untagged Clone

SKU MC201979

Gulo (untagged) - Mouse gulonolactone (L-) oxidase (Gulo), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Gulo
• Synonyms: 5730581M22; AU018375; BC028822; GLO; LGO; sfx; unh; unhip
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028828 sequence for NM_178747; GCCTGAGGTAACCCAGAGCCGAGGTTGCCTGACCACTGCATCTGCTGCTGCCCAGGGCTTTGTTCAACTT CCTGTGGGAACGCTTCAAGTCAAGTCGTCTGACCCTGTTGATCGCTGGAATCATGGTCCATGGGTACAAA GGGGTCCAGTTCCAAAACTGGGCGAAGACCTATGGCTGCAGTCCAGAGATGTACTACCAGCCCACATCAG TGGGGGAGGTCAGAGAGGTGCTGGCCCTGGCCCGGCAGCAGAACAAGAAAGTGAAGGTGGTGGGTGGCGG CCACTCGCCTTCAGACATCGCCTGCACCGATGGCTTCATGATTCACATGGGCAAGATGAACCGGGTTCTC CAGGTGGACAAGGAGAAGAAGCAGGTCACAGTGGAAGCCGGTATCCTCCTGACTGACCTGCACCCACAGC TGGACAAGCATGGCCTGGCCCTGTCTAATCTGGGAGCCGTGTCTGATGTGACGGTTGGTGGCGTCATTGG GTCTGGAACACATAACACCGGGATCAAGCACGGTATCCTGGCCACCCAGGTGGTGGCCCTGACCCTGATG AAGGCTGATGGAACAGTTCTGGAATGTTCTGAGTCAAGTAATGCAGATGTGTTCCAGGCTGCAAGGGTGC ACCTGGGCTGCCTGGGTGTTATCCTCACTGTCACCCTGCAGTGTGTGCCACAGTTCCACCTTCTGGAGAC ATCCTTTCCTTCGACCCTCAAGGAGGTCCTTGACAACCTGGACAGCCACCTGAAGAAGTCTGAGTACTTC CGCTTCCTCTGGTTTCCTCACAGTGAGAACGTCAGCATCATCTACCAAGATCACACCAACAAGGAGCCCT CCTCTGCATCTAACTGGTTTTGGGACTATGCCATTGGGTTCTACCTCCTGGAATTCTTGCTCTGGACCAG CACCTACCTGCCACGCCTCGTGGGCTGGATCAACCGCTTCTTCTTCTGGCTGCTGTTCAACTGCAAGAAG GAGAGCAGCAACCTCAGCCACAAGATCTTCTCCTACGAGTGTCGCTTCAAGCAGCATGTCCAAGACTGGG CCATCCCCAGGGAGAAGACCAAGGAGGCCCTGCTGGAGCTAAAGGCCATGCTGGAGGCCCACCCCAAGGT GGTAGCCCACTACCCCGTGGAGGTGCGCTTCACCCGAGGTGATGACATCCTGCTGAGCCCGTGCTTCCAG AGGGACAGCTGCTACATGAACATCATTATGTACAGGCCCTATGGGAAGGATGTGCCTCGGTTGGATTACT GGCTGGCCTATGAGACCATCATGAAGAAGTTTGGAGGCAGGCCCCACTGGGCAAAGGCCCACAATTGCAC CAGGAAGGACTTTGAGAAAATGTACCCCGCCTTTCACAAGTTCTGTGACATCCGCGAGAAGCTGGACCCC ACTGGAATGTTCTTGAATTCGTACCTGGAAAAGGTTTTCTACTAAAGTAGAAGCAGAAGCAAGCTGCCCT GACCCCTCACTTCTGCTGCCTTGGGGGGGGGGGGCAGGGCAGTGTCTCACAGGCACAGTGGGAGACGACC TCTCCTGAACACAAGGAGGGGCTGGGCTCTGGCCGAGGCCTCTGCCTTCTTTATCATCTTCATAACACCC CAGGCAAGAAGTGGCCTCTCACTCAAATTCCTGTTTGCATCTCCACGAGCCATACATAAACTACAATCAT CTCAGGAAAAGGGGTTCCCCTTGCATCATATCTGTCCAGGCTAAGGATTTGGTCCTTCTAGGTTCTACTG GTCCACCAAGTATAGAGAGATCCCTGGGGCCTGCAGTTTTCCCTCCCTCTTCAGAAGGGATCTCTTGGCA ACAGAGGTAGCATGAGGCATGCTCTGCTTACTTTTATCCTTAAAGGCCTTTCAGATGCCCAGAGTCTGTC TGTTGGTCCTGAGCAAGCCATCTTCCAGATGGGTCCACGTGGCCTTCTGACTGCCATGGCCTGGCCCTCA CAGTGTCTCTTTCGGGTGGTGTTTAGAGTGGAATTTGCCTCGTCTTCTTAACCAGTTCCTGTTAGATCCC TGTGTTTTCTCCCTTCACCTCAGAGACAATTCTTTGGGCTGGGATCTCGCCGTGTCCCTGGGTTTCCCTG GGTCTTGGTTTCATCTTTCTCTTCACAGAGATGATTTCAGTTTATTTGTGGCCTTTCTGGAATGTTCCTT GGAGAGCCAATATGTTCCAGGTACTTTGTCAGAATTAAAAGACTCTGAAAGATAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_178747
• Insert Size: 1323 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC028828, AAH28828
• RefSeq Size: 2240 bp
• RefSeq ORF: 1323 bp
• Locus ID: 268756
• UniProt ID: P58710
• Cytogenetics: 14 D1
• Summary: Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate.[UniProtKB/Swiss-Prot Function]