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Gad1 (NM_008077) Mouse Untagged Clone

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SKU
MC201947
Gad1 (untagged) - Mouse glutamic acid decarboxylase 1 (Gad1), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $553.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gad1
Synonyms EP1; EP10; Gad-; Gad-1; GAD2; GAD4; GAD6
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC027059 sequence for NM_008077
GGAAGCAGCCCTGGGGTGACACCCAGCACGTACTCCTGTGACAGAGCCGAGCCCAGCCCAGCCCCGGGAC GCTTCGCAGAGGAGTCGCGGGAGGGTCCAGCTCGCTGTCGCTGAACCGAGCCTGTTCCTGCGCCCAGTCT GCGGGGGACCCTTGAACCGTAGAGACCCCAAGACCACCGAGCTGATGGCATCTTCCACTCCTTCGCCTGC AACCTCCTCGAACGCGGGAGCGGATCCTAATACTACCAACCTGCGCCCTACAACGTATGATACTTGGTGT GGCGTAGCCCATGGATGCACCAGAAAACTGGGCCTGAAGATCTGTGGCTTCTTACAAAGGACCAATAGCC TGGAAGAGAAGAGTCGTCTTGTGAGCGCCTTCAGGGAGAGGCAGTCCTCCAAGAACCTGCTTTCCTGTGA AAACAGTGACCAGGGTGCCCGCTTCCGGCGCACAGAGACCGACTTCTCCAACCTGTTTGCTCAAGATCTG CTTCCAGCTAAGAACGGGGAGGAGCAAACTGCGCAGTTCTTGCTGGAAGTGGTAGACATACTCCTCAACT ATGTCCGCAAGACATTTGATCGCTCCACCAAGGTTCTGGATTTCCACCACCCACACCAGTTGCTGGAAGG CATGGAAGGCTTTAATTTGGAGCTGTCTGACCACCCCGAGTCTCTGGAGCAGATCCTGGTTGACTGTAGA GACACCCTGAAGTACGGGGTTCGCACAGGTCACCCTCGATTTTTCAACCAGCTCTCTACTGGTTTGGATA TCATTGGTTTAGCTGGTGAATGGCTGACATCGACTGCCAATACCAATATGTTCACATATGAAATTGCACC CGTGTTTGTTCTCATGGAACAGATTACTCTTAAGAAGATGAGAGAGATCGTTGGATGGTCAAATAAAGAT GGTGATGGGATATTTTCTCCTGGGGGAGCCATATCCAATATGTACAGCATCATGGCTGCTCGTTACAAGT ACTTCCCAGAAGTGAAGACAAAAGGCATGGCGGCTGTGCCCAAACTGGTCCTCTTCACCTCAGAACACAG TCACTATTCCATAAAGAAAGCCGGGGCTGCGCTTGGCTTTGGAACCGACAATGTGATTTTGATAAAGTGC AATGAAAGGGGGAAGATAATTCCGGCTGATTTAGAGGCAAAAATTCTTGATGCCAAACAAAAGGGCTATG TTCCCCTTTATGTCAATGCAACCGCAGGCACGACTGTTTACGGAGCATTCGATCCAATCCAGGAAATTGC GGACATATGTGAGAAATACAACCTTTGGCTGCATGTGGATGCTGCCTGGGGTGGTGGACTGCTCATGTCC CGGAAGCACCGCCACAAACTCAGCGGCATAGAAAGGGCCAATTCAGTCACCTGGAACCCTCACAAGATGA TGGGCGTGCTGCTCCAGTGCTCTGCCATTCTGGTCAAGGAAAAGGGTATACTCCAAGGATGCAACCAGAT GTGTGCAGGCTACCTCTTCCAGCCAGACAAGCAGTATGACGTCTCCTATGACACCGGGGACAAGGCGATT CAGTGTGGCCGCCATGTGGACATCTTCAAGTTCTGGCTGATGTGGAAAGCAAAGGGCACCGTGGGATTTG AAAACCAGATCAACAAATGCCTGGAGCTGGCTGATTACCTCTACGCCAAGATTAAAAACAGAGAAGAGTT TGAGATGGTTTTCGATGGTGAGCCTGAGCACACAAATGTCTGTTTCTGGTACATTCCACAAAGCCTTCGA GGGGTTCCAGATAGCCCTGAGCGACGAGAAAAGCTACACAGGGTGGCTCCCAAGATCAAAGCTCTGATGA TGGAGTCAGGAACAACCATGGTGGGCTACCAGCCTCAAGGGGACAAGGCCAACTTCTTCCGGATGGTCAT CTCTAACCCAGCCGCCACCCAGTCTGACATCGATTTCCTCATTGAGGAGATAGAGAGGTTGGGTCAGGAT CTGTAATCCCTCTTTACAGAACCAGAATCATCGGCCATGCTTGTGCCCCTCTGGTACCCTAGAGCACAGC TCTGTCAGTAGCTGACACATCTAGGCCATCTCACTGAAGGAAATTACAATCTCTTAAAGAATATTTGTCA CATTTCACATAAGCTTGTTTGTTAGAGCTAGTGGGGAAATAATGTTCTTTTTAAAAAATTGCACATTAGA AACACAGTATATATGTACAGTTATATATACCTCTCTCTATGTACATGTATGTATAGTGACTGTGGCTTAG TCCTAGACCATAGCATGTTGCTCGTCCCCAGGAAATTAGCCTTACCCCCAGCAATTACTAAGAGGCTAAA CCATCTCGCAAGCAACTACAGGGCGGATGGTATCCCTACTGCAGTGTCCTAGGGACCCAGGGAAAGGCTG TTGGTGGGAGGCTACCTCCCTGCTAGAGCTGTTCCCACGTGAAGGGATGATGGATGACAGATGTACCAGT AAATGACAAATGTCACACCCTCCCTGTTAGTACCCTGCTAGGGGAAATAGTAGCAGTCTTTGTCACAATT GTGCTTTGCTGTGTTTTAGAGATTAATCTGTGTAAACTGTGTACATTGCCATTGTCTATCTTTGGGCAGG GGGAAGTGCATATAATGATTAAATTGTATGTCAGTGAGATATCTGCTTATTTATATTCAAATATATACCA TGTTAAAGAGACATCTTGTATTTTCTTCCCATTTGTAATGTATCTTATTTATATATTAATGGAGTAAGTT CTGGATACTGTTTATGGTATTTTCGTGCATTTGTGAGCCAAAGAGAAAAGATGAAAATTAGTGAGATTTG CATTTATATTAGAGTGCCCTTAACATAATGATTTGAACATATGTGTACTGTCTGGAAAAGAATTCTGATA CTGTACATAGAGTCATGTTATGGAAATCTTGCTTCAGTAGCCTTCGCTCTTCTCTTTCCCCCCTCAGGCT GTATGTCAGATGTTCTCAAAGCTTTTCTAGTAACTGTTGAATAATAATAACTAGATCTCCTGTAATTTTG TAGTAGTATATGACCAATCTCTGTGACTCGCTTAGCTGAAACCTAAGGCAATGTTTCTGAAGACCTCCGA TACACTGACCAGTCCCACAAGTGTTTTTGAAGACATGAAACCCACACTGTGCATTTAGGGTGTGCAAGAA GAATATAAATAAAATAAAAAATATTCTCCATGAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_008077
Insert Size 1782 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC027059, AAH27059
RefSeq Size 3198 bp
RefSeq ORF 1782 bp
Locus ID 14415
UniProt ID P48318
Cytogenetics 2 41.63 cM
Summary The protein encoded by this gene is an enzyme that catalyzes the production of gamma-aminobutyric acid from L-glutamic acid. Alternative splicing of this gene results in two products, the predominant 67 kDa isoform and a smaller 25 kDa isoform. The smaller isoform is not thought to retain catalytic activity. provided by RefSeq, Aug 2015
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (GAD67).
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"NM_008077" in other vectors (6)
SKU Description Size Price
MG209243 Gad1 (tGFP-tagged) - Mouse glutamic acid decarboxylase 1 (Gad1) 10 ug
$489.00 $753.00
MR209243 Gad1 (Myc-DDK-tagged) - Mouse glutamic acid decarboxylase 1 (Gad1) 10 ug
$289.00 $553.00
MR209243L1 Lenti ORF clone of Gad1 (Myc-DDK-tagged) - Mouse glutamic acid decarboxylase 1 (Gad1) 10 ug
$853.00
MR209243L2 Lenti ORF clone of Gad1 (mGFP-tagged) - Mouse glutamic acid decarboxylase 1 (Gad1) 10 ug
$853.00
MR209243L3 Lenti ORF clone of Gad1 (Myc-DDK-tagged) - Mouse glutamic acid decarboxylase 1 (Gad1) 10 ug
$853.00
MR209243L4 Lenti ORF clone of Gad1 (mGFP-tagged) - Mouse glutamic acid decarboxylase 1 (Gad1) 10 ug
$853.00

file-alt Citations

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