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Dnajb12 (NM_019965) Mouse Untagged Clone

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SKU
MC201823
Dnajb12 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dnajb12
Synonyms Dj10; mDj10
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC034162 sequence for NM_019965
CCGGCCTGCCCCGCGACGCGCCGGCGGCTGGCGCAGCCCTTCGCTCGCCCGGCCTCCCCCTCCCTGGTTC CGTGCTCGGGTTCCGCCATGGAATCCAACAAGGATGAAGCCGAGCGCTGTATCAGCATCGCCCTCAAGGC CATCCAAAGCAACCAGCCCGAGCGGGCGCTGCGCTTCCTGGAGAAGGCGCAGCGGCTCTACCCGACGCCA CGAGTCAGCGCACTGATCGAGTCCCTCAACCAAAAACCACAGTCCACCGGTGATCACCCTCAACCCACAG ACACTACCCATACCACCACCAAGAAGGCAGGCGGGACCGAAACCCCCTCAGCCAACGGCGAGGCAGGAGG AGGGGAGAGCGCCAAGGGCTATACCTCAGAGCAGGTGGCAGCTGTGAAAAGGGTCAAGCAGTGTAAAGAT TACTATGAGATCCTTGGGGTAAGCAGAAGTGCCTCAGATGAGGACCTAAAGAAGGCCTACCGCAAGCTGG CCCTCAAGTTCCATCCAGACAAGAACCATGCGCCTGGGGCCACTGAAGCTTTCAAAGCCATTGGCACAGC GTATGCCGTACTCAGCAACCCAGAGAAAAGGAAACAGTACGACCAGTTTGGTGATGACAAGAGCCAGGCT GCCCGGCACGGCCACAGCCACGGGGACTTCCACCGAGGCTTTGAGGCTGACATCTCCCCAGAAGACCTCT TTAACATGTTCTTTGGTGGCGGCTTTCCTTCCAGTAACGTTCATGTCTACAGCAATGGCCGAATGCGGTA CACCTACCAGCAGAGGCAAGACCGCAGAGACAACCAGGGTGATGGCGGGCTAGGAGTGTTCGTGCAGCTG ATGCCCATCCTCATCCTCATCCTCGTGTCTGCGCTCAGCCAGCTCATGGTGTCCAGTCCTCCCTACAGCC TGAGCCCGAGACCGTCAGTGGGCCACATCCACAAGCGAGTCACTGACCACCTGAACGTCGCCTACTACGT GGCAGACACCTTCTCCGAAGAGTACACGGGCTCCAGCCTCAAAACAGTGGAACGGAATGTAGAGGATGAC TACATCGCCAACCTGCGCAACAACTGCTGGAAGGAGAAGCAGCAGAAGGAAGGCTTGCTGTACCGAGCCC GCTACTTTGGTGACACAGACATGTACCACAGAGCACAGAAGATGGGCACCCCGAGTTGCAATCGGCTGTC AGAGGTGCAGGCCTCCCTGCATGGATAGTTCTGGGCCAGCCACACCACCCAGGTCCAAACTATGAAATCC CTGGAGAATTTTTGGTGACACACACTGAGCCAGCCACAGTGATGGACTGTATATTTAAGGAAAGACATAC ACAGAAAAAATTTAAATGGAACTGGAAGCCAAACACCGCACAGCCATCCTCTCTCAGACAGTGAATGCAG AAAGCTCTTAGGACAGACAGACAGACAGCCTACCTCGGGCTGCTTCCTCCTCTCCCAGGGCAGGCAAGAA GCTCCGCATCACTGGCTCCTAGGATACCGAAACCATGGCAACGAAAGTAGAATGTAAAACTTGCAGCAAA TGTCTGTGGGGAAGGACTGGGGGAGGGGACACCCCACTGTGTCTCCTCCCTCCTCCCTGAGCTGCCACCA GTCACTCAAGGAGAAATCAGGCTGAGGCATGGGCCAAGGAACAGAGAGGAGAGAATTCCAGAGAACGTAA CTTAGAAATGCGTATGTATGTATATATATATCTATTTATATGTAAATAGACATATATAAAGATATATATA TATATATAAAGTCTTTTAAAACTAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_019965
Insert Size 1131 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC034162, AAH34162
RefSeq Size 1789 bp
RefSeq ORF 1131 bp
Locus ID 56709
UniProt ID Q9QYI4
Cytogenetics 10 B4
Summary Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway. Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities. Can also act independently of HSPA8/Hsc70: together with DNAJB14, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers. While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70. When overexpressed, forms membranous structures together with DNAJB14 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear.UniProtKB/Swiss-Prot Function
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"NM_019965" in other vectors (5)
SKU Description Size Price
MC201824 Dnajb12 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12), (10ug) 10 ug
$457.00
MG205857 Dnajb12 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12) 10 ug
$657.00
MR205857 Dnajb12 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12) 10 ug
$289.00 $457.00
MR205857L3 Lenti ORF clone of Dnajb12 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12) 10 ug
$757.00
MR205857L4 Lenti ORF clone of Dnajb12 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12) 10 ug
$757.00

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