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Micu2 (NM_028643) Mouse Untagged Clone

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SKU
MC201768
Micu2 (untagged) - Mouse EF hand domain family A1 (Efha1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Micu2
Synonyms 1110008L20Rik; 4833427E09Rik; AU041871; Efha1; Smhs2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC031172 sequence for NM_028643
CGGACGCGTGGGCGCTTCCGCAAAGATGGCGGCGGCTGCGGGAAGAAGCGCTTGGCTGGCGGCCTGGGGC GGAAGGCTGCGGCGCGGGCTCGCGGCCGGCCGACGAGCTGTGCCGACTCGCGGCCCTCTCGCAGCGGCTG TGGCCGGGGTAGCCCTGGCAGGCGCAGGAGCGGCATGGCATCACGGCCGCGTGAAAGCAGCCGCGCGCGA GGGCTCCCGAACGGTGTCGGCGCAGAAAAATTATTTGGGACCAATAGAGAAACTTTCTCTCCGCAAACAG CGGTTCATGCAGTTTTCATCCCTGGAGCACGACGGAGAGTATTACATGACACCCCGAGACTTCCTCTTCT CAGTCATGTTTGAGCAAGTGGAGCGTAAAACACTGGTCAAGAAGCTGGCAAAAAAGGATATCGAGGATGT ACTGTCAGGAATCCAAACAGCACGCTGTGGATCAACATTTTTTAGAGACCTGGGTGATAAAGGGGTAATT TCATATACCGAGTATCTTTTCTTGCTTACAATCCTCACAAAACCTCACTCTGGGTTCCATGTTGCTTTTA AAATGCTGGATGTGGATGGGAATGAGATGATCGAGAGGAAGGAGTTTGTTAAGCTGCAGAAGATCATAAG TAAACAAGATGGCTTCAAGACAGTGAAGACTAATGAGACAGAATACCAGGACCCAACAGTGAAAGAGCCT GGCGTTAACACAACCCTTCAAGTGCGTTTCTTTGGGAAAAGAGGAGAAAAAAAACTCCATTATAAAGAAT TTCGAAGATTTATGGAAAATTTACAAACAGAAGTTCAAGAAATGGAATTCCTTCAGTTCTCTAAAGGCCT GAATTTCATGAGAAAAGAAGACTTTGCAGAATGGCTACTTTTCTTCACTAACACTGAAAATAAAGATATT TACTGGAGAAACGTGAGAGAAAAGTTGTCAGTAGGAGAGAGCATTAGTTTGGATGAGTTCAAGTCCTTTT GCCATTTTACAACGCATTTGGAAGACTTTGCTATCGCCATGCAAATGTTTAGTTTAGCTCATCGCCCTGT GCGGCTGGCAGAGTTCAAGAGAGCTGTGAAGGTAGCGACTGGACAGGAGCTCTCGGACAATCTTCTGGAC ACCGTCTTCAAGATCTTTGACCTGGACGGGGACGAGTGCCTCAGCCACGGCGAGTTCCTTGGGGTACTGA AAAACAGGATGCACCGAGGCTTATGGGTGTCACAGCAGCAGAGTGTGCAAGAATACTGGAAGTGTGTGAA GAAGGAAAGCATCAAGGGAGTAAAGGAAGCCTGGAGACAACAAGCCGGCAAGGGCCCCTTCTAAGGGGAA GCTGATGGAGCTCCAGGAGCCAGAAAGTGGTTTTTCCAAGAGCTGACTGTTTCTATGTCTTCATTGAGTT TCTTTTGAGTGTGAAGATGTGAAGACTCACTTTCTGATTTGGGGGATTTGGGAGGGATGACGGGAGGTGA ATAAGTGTGTGCCACAGTGGGAAGTCAGAGGCCAGCCTGTAGGAGCTGGTTCTCTCTGTCTACCATGGGA GTCCCAGAGATTGAACTCAGGTCTCGAGCTTTGACATCAAGTGCCTTTACCTGATGAGCCATCTCACCAG CTCCGACTCCATCATTTCTTGGTGAATTTTCATTACACAAAGTATTCCTTTATGAAAATACACACTGCTC TATTAAAGACTTGTGTACAAAGTGTGCTTTCTGGAGGGCTAAACAAGTCAGGGAAGGAACTTCATTCCCA AGACAGGTACTGATCCCAGAAGCCGTGATAGCTTAGGGAATACTTGCAGTTAAAGGCTTGCTCCAGTGTT CTTTGCTCCCTGCCTTGAAATAGCACATAATGGCAGTGTCCGGTCTGGCAGCAGGATGAAGTATTTACAT TGATACAAAAAAATTTTTTCTTTTTTAAATGTTCCATGCTTTTGAAATATAAATAGAGTGTCCTCTCCCA GACACTCCCAGGGGAGCTCAGCCAGCAAAGGACACAGCTGTGTACCATGCTTTCCTAACAAGCAGCACAC AGAGTTCCATCTAAAACCACTCATACACACACAGATCACACACAGATCACACACATACAGTGATCATAAT AGCAAGTTTTCTTAAGCTACGGGATCTTGGGTTGTGGGAAATATGGCACTATAAAGTAGCTTGCAATAAA ATAAATAGGAAATAATGTTATGTGGAGATGGACATGTGTATGTATACAGGTGGACATACATGTGTGTGTG GAAGCCCCTGCTCCTGTAAATACCCCCATGTTTCTGAAGTGATCCCAATAAACCCATGAGCTCACCAAGC TAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_028643
Insert Size 1299 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC031172, AAH31172
RefSeq Size 2333 bp
RefSeq ORF 1299 bp
Locus ID 68514
UniProt ID Q8CD10
Cytogenetics 14 C3
Summary Key regulator of mitochondrial calcium uniporter (MCU) required to limit calcium uptake by MCU when cytoplasmic calcium is low (PubMed:23409044, PubMed:24560927). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulate and inhibit MCU activity, depending on the concentration of calcium (PubMed:24560927). MICU2 acts as a gatekeeper of MCU that senses calcium level via its EF-hand domains: prevents channel opening at resting Ca(2+), avoiding energy dissipation and cell-death triggering (PubMed:24560927).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028643" in other vectors (4)
SKU Description Size Price
MG206892 Efha1 (tGFP-tagged) - Mouse EF hand domain family A1 (Efha1) 10 ug
$657.00
MR206892 Efha1 (Myc-DDK-tagged) - Mouse EF hand domain family A1 (Efha1) 10 ug
$289.00 $457.00
MR206892L3 Lenti ORF clone of Efha1 (Myc-DDK-tagged) - Mouse EF hand domain family A1 (Efha1) 10 ug
$757.00
MR206892L4 Lenti ORF clone of Efha1 (mGFP-tagged) - Mouse EF hand domain family A1 (Efha1) 10 ug
$757.00

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