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Btg1 (NM_007569) Mouse Untagged Clone

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SKU
MC201025
Btg1 (untagged) - Mouse B-cell translocation gene 1, anti-proliferative (Btg1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Btg1
Synonyms AI426953; AW546738
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC018309 sequence for NM_007569
CGGAGCTGGAAATGCAACTCTCGGGATCCTCGGAGGCTACCGAGCTGGAGGCGGAGGCGGCTGGGGAGGT CCGAGCGATGTGACCAGGCCGCCATCGCTCGTCTCTCTCTGTCTCTCCTGCCGCCTCCTGGCTTGAAAAT AACTTTTTTACTCTAAAGGAAAGAAAAAAAATCCCCTAGAACCAGTAGCCGTCCGCGCCTCGCGCCCTCG CTTCCTCTCGGCCGTCGGGTCTGGGCGAGAGCCCGGGGTGGCCGCTTCGCCGTCGGGGCCGAGCCCCGGC CGCCCCGGGCCACCCCCGGCCGCCGCCCCCATGCATCCCTTCTACACCCGGGCCGCCACCATGATAGGCG AGATCGCCGCCGCGGTGTCCTTCATCTCCAAGTTCCTCCGCACCAAGGGGCTCACGAGCGAGCGACAGCT GCAGACTTTCAGCCAGAGCCTGCAGGAGCTGCTGGCAGAACATTACAAACATCACTGGTTCCCAGAAAAG CCGTGCAAGGGATCAGGTTACCGTTGTATTCGCATCAACCATAAGATGGATCCTCTGATTGGACAGGCAG CCCAGCGGATTGGACTGAGCAGTCAGGAGTTGTTCAGGCTTCTCCCAAGTGAACTCACACTCTGGGTTGA CCCCTACGAAGTGTCCTACCGGATTGGAGAGGATGGCTCCATCTGCGTGCTGTACGAAGCCTCACCAGCA GGAGGTAGCACTCAAAACAGCACCAACGTGCAAATGGTAGACAGCAGAATCAGCTGTAAGGAGGAACTTC TCTTGGGCAGAACAAGCCCTTCCAAAAACTACAATATGATGACTGTATCAGGTTAAGATATAGTCTATTG GATGGATCATCTTATAATGGATGGATGAATTTGATTTTTTTTGCTTTGGGTGGGCTCCTCTTGGGGATGG ATTATGGAATTTAAACCATGTCACAGCTGTGAAGATCTGGCACAAGATAGAGTGGTAATAATTTTTTTAT TTCTTTCTTTTTTTTTTTTTTTTAATTTAGTGACAGTGCCATAGTTTGGACAGTACCTTTCAGTGATTTA AATAGCCTGTGAGTCCAAGGAAAGGATCACTTTATTTGCTAGGGAGTGAAGTCGAAGGGTGGTTTCAGTT TCTCCCAGACCTTATACCCAATTTGTCACAGCAGTCCCTTTAAGGAAATTCTGTCTTTCAAAGAACCCTC TTTCGCAGTCAACCTTGCAGGGGAATTTGCACTATTACACTTGAAAGTTCTCAGTAACTTTTTTGGCAGC TCAATAGGAAAGCTCAATGTTTTAAGCATGGTAGTACTGGAAAAATTTTTACACGGAGACTTTTACCTAG CACTTAAAATGTATAAATGTACATAAAGACAAACTTAGTACGCATGACCTGGGGGAAATGGTCAGACCTT GTGTTTTTGGCGTTGAGAGTAGGGAGCGACCAGGCTCTGCCATGGCAGCAGGCTTTGAAAAGACCCTTCA AAAAGACACTGTCTCAACTGTGGTTGTTAGCACCAGCCAGCTCTCTGTACATTTGCTAGCTTGTAGTTTT CTAAGACTGAGTGGAGTAAACTTCTTATTTTTAGAAAGTGGAGGTCTGGTTTGTAACTTTCCTTGTACTC AATTGGGTAAGAGTCTTTCCCACAAACCACCATCTATTTTGTGAACTTTGTTAGTCATCTTTTATTTGGT AAATTATGAACTGGTGTAAATTTGTACAGTTCATGTATATTGATTGTGGCAAAGTTGTACAGATTTCTAT ATTTTGGATGAGAAATTTTTCTTCTCTCTATAATAAATTGTTTCTTATCTTGGCATTTTAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_007569
Insert Size 516 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC018309, AAH18309
RefSeq Size 1841 bp
RefSeq ORF 516 bp
Locus ID 12226
UniProt ID P62325
Cytogenetics 10 C3
Summary Anti-proliferative protein.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007569" in other vectors (4)
SKU Description Size Price
MG201435 Btg1 (tGFP-tagged) - Mouse B-cell translocation gene 1, anti-proliferative (Btg1) 10 ug
$500.00
MR201435 Btg1 (Myc-DDK-tagged) - Mouse B-cell translocation gene 1, anti-proliferative (Btg1) 10 ug
$289.00 $300.00
MR201435L3 Lenti ORF clone of Btg1 (Myc-DDK-tagged) - Mouse B-cell translocation gene 1, anti-proliferative (Btg1) 10 ug
$600.00
MR201435L4 Lenti ORF clone of Btg1 (mGFP-tagged) - Mouse B-cell translocation gene 1, anti-proliferative (Btg1) 10 ug
$600.00

file-alt Citations

The tumor suppressor BTG1 is expressed in the developing digits and regulates skeletogenic differentiation of limb mesodermal progenitors in high density cultures
Lorda-Diez, Montero, Garcia-Porrero et al
Cell Tissue Res. (2015)
Cited by 6
AI Summary: OriGene's mouse Btg1 gene construct (MC201025) cloned into the pCMV6-XL5 vector was used for gain-of-function experiments to investigate the role of BTG1 in regulating skeletogenic differentiation of limb mesodermal progenitors.
Snippet: For gain-of-function experiments, skeletal progenitors were electroporated with a construct of mouse Btg1 gene (MC201025) cloned into the pCMV6-XL5 vector (Origene) employing the Multiporator System (Eppendorf) and cultured in high-density conditions as ... Cite SaveSaving
Showing 1-1 of 1 papers.
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