Trib3 (BC012955) Mouse Untagged Clone

SKU MC200945

Trib3 (untagged) - Mouse tribbles homolog 3 (Drosophila) (cDNA clone MGC:18731 IMAGE:3980838), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Trib3
• Synonyms: Trb3, Nipk, SINK, SKIP3, TRB-3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC012955; CGGGGATCCGAGCCCGCGTCCCGGTGCCGCAGCACTTTAGCAGCGGAAGAGGCTGCTGCCGAGTACGTGA CGGCTGGGGTCTGGCACTAGCGTGCAGGAGACTCCGAGAGCTGCTCAGTTAGGCTGGCGGCGGTCCACGG GCGTGGGCGCAGGCGGCTCTGAGGCTCCAGGACAAGATGCGAGCTACACCTCTGGCTGCTTCTGCTGATG TTTCCTGCAGGAAGAAACCGTTGGAGTTTGATGACAATATTGATGCCAAGTGTCCAGTCCTAAAACGAGT GAGAGATGAGCCTGAGCCCGGACCACTCCCCAGCCTGCTGCCCCCCAGCCCACCTCCCGCCTCAGACTTG TCACCTGCTGTGGCCCCTGCAACTCGACTGGGGCCTTATATCCTTTTGGAACGAGAGCAAGGCAGCTGCA GCTATCGAGCCCTGCACTGCCCCACAGGCACAGAGTACACCTGCAAGGTGTACCCTGCCAGCGAGGCCCA GGCGGTGCTGGCACCTTATGCCCGGCTGCCTACCCACCAGCATGTGGCCCGTCCCACAGAGGTCCTGCTG GGCTCTCGGCTCCTTTACATCTTTTTCACGAAGACCCATGGGGACTTGCACAGCCTGGTGCGCAGCCGCC GCGGTATCCCAGAGCCCGAGGCTGCCGGGCTCTTCCGGCAGATGGCTAGTGCCGTGGCACACTGCCACAA GCACGGGCTTGTCTTGCGCGACCTCAAGCTGCGTCGCTTTGTCTTCAGCAACTGTGAGAGGACGAAGCTG GTGCTGGAGAACCTGGAAGATGCCTGCGTGATGACTGGATCAGATGACTCTCTGTGGGACAAGCATGCGT GCCCTGCCTACGTGGGACCAGAGATACTCAGCTCCCGGCCATCCTACTCTGAGCCAGTCCTGCTCTTTGG CAAGATCCGTAGAGGGACCTTTGCCCTGCCTGAGGGCCTATCAGCCCCAGCCCGCTGTCTGATCCGCTGT CTCCTCCGCAAGGAACCTTCAGAGCGACTTGTGGCCCTGGGAATCCTCTTGCATCCCTGGTTGAGAGAGG ATCACGGCCGAGTCTCTCCTCCACAGTCTGACCGAAGGGAGATGGACCAGGTGGTCCCAGATGGGCCACA GCTGGAGGAGGCTGAGGAAGGGGAGGTGGGGCTGTACGGCTAGGGCCACCTTACTGGCCCCTCAGCTCCA AGGTGTGAGTTGAGTACCTGAGTCTCAGCTTTCTCCTGACTTTTTGGGCCAAGCTAAACCTTAAGTGCCT TTCTGGAGGAAGAAACAGCCGGCGTGCCTTATTCGTTCCTGTGCCTAGTGGGGTGATGCTCCCTGAGTGC CACGCCCTGCTCTAGGTGCTGTGAACAGCAAAGGAAAAAGAGGGAGAAATATACCTTGCTGGCCAAGTTG CCACAGTGCCGCATGCTCTCCGGCCAGCCCCTGCCTTGGGCACGTTTCCTACCGGGGCTGTCTTCTCTGG TGCTGGTTCATGGGACAGTCCCTTTTATCAGTGCCCCAGCTGCCTTCTGTCGTGTGCCTTTAAACAAAGG AAACATCTCTGTGCCAAAGACCAAAGTCCTCACTCACCATGTGCCTCAGGACCACAAATCAGGCCGGGCT GGGATTGTGGCCTCCAGGCAGGAGAACCTGTTTGAAGAAGACTTGGCTGTGGGATTCAAGCCAAAAGGAA GAGACTCCAATAAAGAGGCATTTGACTCAAGGAATAGTCTGGAATAAGGATCCGGGTCTGTTTACCCTGG TAGCCTCCGGCTGAAGCACCAAAGTTGGAGGGGGAGGACCAGGCAGGGTCTGTCCTACTGCCTACAGACA ATCTCAGGCCCTGTTTTGGGGACTTTTAGTACCCAGGCCCACAGTCAGTTTGATGACTTTGGTCTGGTTA AGTGTGTATTTTCATCATGTGCCCAATAAAAGAGAGCTGTGTGGTTTGATATGTGAAAAAAAAAAAAAAA AAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC012955
• Insert Size: 987 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC012955, AAH12955
• RefSeq Size: 1969 bp
• RefSeq ORF: 987 bp
• Locus ID: 228775
• Cytogenetics: 2 74.83 cM
• Summary: Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (By similarity). Can inhibit APOBEC3A editing of nuclear DNA.[UniProtKB/Swiss-Prot Function]