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Nit1 (NM_012049) Mouse Untagged Clone

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SKU
MC200930
Nit1 (untagged) - Mouse nitrilase 1 (Nit1), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nit1
Synonyms AI255805; ESTM30; W57327
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC021634 sequence for NM_012049
CCACGCGTCCGGGCAGCTCTTGAGATCCGACCCCGTGAATGGTTTTGGCTGTGTCTTCATGCTGGGCTTC ATCACCAGGCCTCCTCACCAACTCCTGTGTACCGGATACCGATTACTTCGAACCCCAGTACTTTGTACTC AGCCCAGGCCCAGAACCATGTCCTCATCAACTTCCTGGGAGCTGCCCCTGGTGGCTGTGTGCCAGGTAAC ATCAACACCAAACAAGCAAGAGAACTTTAAAACATGTGCTGAGTTGGTTCAAGAGGCTGCCAGACTGGGT GCTTGCCTGGCCTTTCTGCCTGAGGCATTTGACTTTATTGCACGAAACCCTGCCGAGACATTACTCCTGT CCGAACCACTGAATGGGGATCTTTTGGGCCAATATAGCCAGCTTGCCAGGGAATGTGGAATCTGGCTGTC CTTGGGCGGTTTCCACGAGCGTGGCCAAGACTGGGAGCAGAATCAGAAAATCTACAATTGTCATGTGCTT TTGAACAGCAAGGGATCAGTAGTGGCCAGTTACAGGAAGACACATCTGTGCGATGTAGAGATCCCAGGTC AGGGGCCGATGAGAGAAAGCAACTATACCAAGCCTGGAGGCACTCTTGAGCCACCTGTCAAGACACCGGC TGGCAAGGTTGGTCTAGCAATCTGTTATGACATGCGGTTCCCTGAACTTTCTTTGAAATTGGCTCAAGCT GGGGCAGAAATACTTACTTATTCTTCAGCCTTTGGATCTGTTACAGGTCCAGCCCACTGGGAGGTGCTGC TGCGGGCCCGCGCCATTGAATCTCAGTGCTATGTAATAGCAGCAGCGCAGTGTGGACGCCACCATGAAAC AAGAGCAAGTTATGGCCATAGCATGGTGGTTGACCCGTGGGGCACAGTGGTGGCCCGCTGCTCCGAGGGA CCAGGCCTCTGCCTTGCTCGAATTGATCTCCACTTTCTACAACAGATGCGCCAACACCTGCCTGTGTTCC AGCACCGCAGACCTGACCTCTATGGCAGTCTGGGTCATCCACTCTCTTAATGCTTTTAACTTCTGTTGGT TAAGACACAGACACAGCCAACTGGGAGGTGATGCTACTGTGACTTGTAGGCAGGACACAAGCACGGTTCC CTTCACTTGCAGAACTTTAACCTCTTTTAATGGAACACAGGCTTAATCAAGTCCATGGGAAAGAAGAGAC TTTGACCTGACCTCAGATTTCAGTTTCAGAAAAGTGGGATTTTATATAGTCACTGTTTATTTCATGAAAA CTGAAGTTATGCTGAGGGCTGAGCAGCACTGGCATTGAAAAAAAAATTTATTATTCTTAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_012049
Insert Size 972 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC021634, AAH21634
RefSeq Size 1365 bp
RefSeq ORF 972 bp
Locus ID 27045
UniProt ID Q8VDK1
Cytogenetics 1 79.35 cM
Summary Catalyzes the hydrolysation of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_012049" in other vectors (4)
SKU Description Size Price
MG204685 Nit1 (tGFP-tagged) - Mouse nitrilase 1 (Nit1) 10 ug
$500.00
MR204685 Nit1 (Myc-DDK-tagged) - Mouse nitrilase 1 (Nit1), transcript variant 1 10 ug
$289.00 $300.00
MR204685L3 Lenti ORF clone of Nit1 (Myc-DDK-tagged) - Mouse nitrilase 1 (Nit1), transcript variant 1 10 ug
$600.00
MR204685L4 Lenti ORF clone of Nit1 (mGFP-tagged) - Mouse nitrilase 1 (Nit1), transcript variant 1 10 ug
$600.00

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