Tsg101 (NM_021884) Mouse Untagged Clone

SKU
MC200893
Tsg101 (untagged) - Mouse tumor susceptibility gene 101 (Tsg101), (10ug)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tsg101
Synonyms AI255943; CC2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC005424 sequence for NM_021884
CCCACGCGTCCGCGGCGATTGTCTGGCGGCCTGGGGCGGCCCCGTGGCGCCTACCCTCTGCCTGTGGGGA CGGAGGAGCGCGCCATGGCGGTGTCCGAGAGTCAGCTGAAGAAGATGATGTCCAAGTACAAATATAGAGA TCTAACCGTCCGTCAAACTGTCAATGTCATCGCTATGTACAAAGATCTCAAACCTGTATTGGATTCATAT GTTTTTAATGATGGCAGTTCCAGGGAGCTGGTGAACCTCACTGGTACAATCCCAGTGCGTTATCGAGGTA ATATATATAATATTCCAATATGCCTGTGGCTGCTGGACACATACCCATATAACCCCCCTATCTGTTTTGT TAAGCCTACTAGTTCAATGACTATTAAAACAGGAAAGCATGTGGATGCAAATGGGAAAATCTACCTACCT TATCTACATGACTGGAAACATCCACGGTCAGAGTTGCTGGAGCTTATTCAAATCATGATTGTGATATTTG GAGAGGAGCCTCCAGTGTTCTCCCGGCCTACTGTTTCTGCATCCTACCCACCATACACAGCAACAGGGCC ACCAAATACCTCCTACATGCCAGGCATGCCAAGTGGAATCTCTGCATATCCATCTGGATACCCTCCCAAC CCCAGTGGTTATCCTGGCTGTCCTTACCCACCTGCTGGCCCATACCCTGCCACAACAAGCTCACAGTACC CTTCCCAGCCTCCTGTGACCACTGTTGGTCCCAGCAGAGATGGCACAATCAGTGAGGACACTATCCGTGC ATCTCTCATCTCAGCAGTCAGTGACAAACTGAGATGGCGGATGAAGGAGGAAATGGATGGTGCCCAGGCA GAGCTTAATGCCTTGAAACGAACAGAGGAAGATCTGAAAAAAGGCCACCAGAAACTGGAAGAGATGGTCA CCCGCTTAGATCAAGAAGTAGCTGAAGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACT AAGTTCTGCTCTGGAGAAAATGGAAAATCAATCTGAAAATAATGATATTGATGAAGTTATCATTCCCACA GCCCCACTGTATAAACAGATTCTAAATCTGTATGCAGAGGAAAATGCTATTGAAGACACTATCTTTTACC TTGGAGAAGCTTTGCGGCGGGGAGTCATAGACCTGGATGTGTTCCTGAAACACGTCCGCCTCCTGTCCCG TAAACAGTTCCAGCTAAGGGCACTAATGCAAAAGGCAAGGAAGACTGCGGGCCTTAGTGACCTCTACTGA CATGTGCTGTCAGCTGGAGACCGACCTCTCCGTAAAGCATTCTTTTCTTCTTCTTTTTCTCATCAGTAGA ACCCACAATAAGTTATTGCAGTTTATCATTCAAGTGTTAAATATTTTGAATCAATAATATATTTTCTGTT TCCTTTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_021884
Insert Size 1176 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC005424, AAH05424
RefSeq Size 1463 bp
RefSeq ORF 1176 bp
Locus ID 22088
UniProt ID Q61187
Cytogenetics 7 B3
Summary Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
Resources
"NM_021884" in other vectors (4)
SKU Description Size Price
MG206104 Tsg101 (tGFP-tagged) - Mouse tumor susceptibility gene 101 (Tsg101) 10 ug
$657.00
MR206104 Tsg101 (Myc-DDK-tagged) - Mouse tumor susceptibility gene 101 (Tsg101) 10 ug
$457.00
MR206104L3 Lenti ORF clone of Tsg101 (Myc-DDK-tagged) - Mouse tumor susceptibility gene 101 (Tsg101) 10 ug
$757.00
MR206104L4 Lenti ORF clone of Tsg101 (mGFP-tagged) - Mouse tumor susceptibility gene 101 (Tsg101) 10 ug
$757.00

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