Ggh (NM_010281) Mouse Untagged Clone

SKU MC200869

Ggh (untagged) - Mouse gamma-glutamyl hydrolase (Ggh), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Ggh
• Synonyms: gamma-GH
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC009809 sequence for NM_010281; CCACGCGTCCGCGATGGCGAACCTGGGCTACCTGCTGTGCTTGTTGGGTTTGCTACTATGCGGGCTTTCA AGTCCCGGGATGTCCAGACCCTACAACCATGGCTCCGAGAGGCCCATCATCGGAGTAGTAATGCAAGAAT GTTTTGGGAAAATGGCGAAGTTGGGAAACTACTACATTGCTGCATCCTATGTGAAGTATATAGAGTCTGC AGGTGCACGAGTTGTGCCCATAAGGCCTGATCTTAGTGATGCAGAGTATGAAGAACTTTTTAGATCTATT AATGGAGTTCTCCTTCCTGGAGGAGGTGCTAACCTAACAGATTCAGGTTATTCCCGAGTGGCCAAAATAT TTTTCAGCAAGGCTCTAGAGAGCTTTGATAATGGAGGCCATTTTCCTGTGTGGGGAACATGCCTTGGATT TGAGGAGCTTTCTGTCCTGGTTAGTGGAGAGAACTTACTAACGAGTACAGACACTAAGTCAAAGAAATTG CCCTTGAACTTCACTGAAGGTGCAAGGAAGAGCAGAATGTTCAAACATTTTCCTACTGAGTTATTGGATT CATTAGCACTAGAAAATCTGACCGCAAATTTTCACAAATGGAGCCTCTCTGTGAAGAATTTTACAGAAAA TGAAAAGTTAAAGAAGTTTTTCAATATATTAACAACCAATACAGATGGCAAAACTGAGTTCATTTCATCC ATGGAAGGATTTAAGTATCCAGTGTATGCTGTCCAGTGGCATCCTGAGAAAGCACCATTTGAGTGGAAGA ATCTGGGCGGCATTTCTCATGCACCAAATGCTGTGAAGACTTCATTCTACTTAGCAGAATTTTTAGTTTC TGAAGCTCGGAAGAACAGTCACCACTTTGAGAACGTGGTTAAGGAGACGGCATCCTTGATTTACAAGGTC TATCCCATTTATACTGGAAATATTTCTTCATTTCAGCAAGCTTATATGTTTGACTGAAATTCTTCAATAT GGTAACCCAGCAGCTTTGAGTAATTCTGTGATTGAATTGCTAACACAGCTCAGGGCAGTGTGAGAAAGCC ACACAGATATCTTTGCTGTGTGCCTGGTTCTGATTCCTGGCTTAATACATGATTGTTTTTATCAGATTTG ATAACCTGACAATGAAAAATATAAAAAATCAGAGTGTTTTTCATGAAAATGCCTTCTTATGTCTGAAGAT TCTAAAATACAAGTTTTTGAAAATAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_010281
• Insert Size: 954 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC009809, AAH09809
• RefSeq Size: 1231 bp
• RefSeq ORF: 954 bp
• Locus ID: 14590
• UniProt ID: Q9Z0L8
• Cytogenetics: 4 A3
• Summary: Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Acts as endopeptidase. Lysosomal enzyme is activated by sulfhydryl compounds.[UniProtKB/Swiss-Prot Function]