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Ess2 (NM_001081633) Mouse Untagged Clone

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SKU
MC200734
Dgcr14 (untagged) - Mouse DiGeorge syndrome critical region gene 14 (Dgcr14), transcript variant 2, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ess2
Synonyms AI462402; D16H22S1269E; Dg; Dgcr1; Dgcr14; Dgsi; ES; ES2; Es2el
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC013711 sequence for NM_001081633
CGGACGCGTGGGTGGGATAGCGATGGGGACGCCCGGGACCTCGGCGGGTGCTCTGTTTCTGTCCTCCGCG TCCGCGCCCTCAAGGAAGCGCGCGGCTGGGGAGGCTGGAGAGGCCGGAGTTGCGAGAAGCAGGCAGCGGG TCCTGGATGAAGAAGAGTACATCGAGGGACTTCAGACAGTTATCCAGAGAGACTTCTTCCCTGATGTGGA GAAGCTACAGGCACAGAAGGAGTACCTGGAGGCCGAGGAAAACGGAGATTTGGAGCGCATGCGCCAGATT GCCATCAAGTTTGGCTCTGCCCTGGGCAAGATATCTCGGGAACCTCCACCACCCTATGTTACTCCAGCCA CCTTTGAAACTCCTGAGGTACACCCAGGCTCTGCTGTGCTGGGCAACAAGCCCCGGCCCCAGGGCCGAGA CCTAGATGATGGAGAGGCTGGAGAGGAAGAGGAGAAGGAGCCACTGCCCAGCCTGGATGTCTTCTTGAGC CAGTACACAAGTGAGGACAATGCCTCCTTCCAGGAGATCATGGAGGTGGCCAAGGAAAAAAGCCATGCAC GGCACGCGTGGCTCTACCAGGCTGAGGAGGAATTTGAGAAGCGACAGAAAGATAATCTTGAACTCCCATC GGCAGAGCATCAAGCCATTGAGAGCAGTCAGGCTGGAGTGGAGACCTGGAAGTACAAGGCCAAGAACTCT CTCATGTACTATCCCGAGGGTGTCCCTGATGAAGAGCAGTTGTTTAAGAAGCCGAGACAGATAGTACATA AGAACACACGTTTCCTCCGAGATCCCTTCAGTCAGGCTTTGAGCAGGTCCCAGCTTCAGCAGGCGGCTGC CCTCAATGCCCAGCACAAACAGGGCAAGGTCGGCCCTGACGGCAAGGAACTCATTCCCCAGGAGTCCCCC AGAGTGGGCGGCTTTGGATTTGTTGCCACTCCTTCTCCTGCTCCTGGTGTGAATGAGTCCCCACTGATGA CATGGGGAGAGGTTGAGAACACGCCCCTGCGAGTAGAAGGATCCGAGAGCCCCTATGTGGACAGGACACC TGGACCCACATTCAAGATCTTGGAGCCAGGACGCAGGGAGCGTCTGGGCCTGAAGATGGCCAATGAAGCG GCTGCCAAAAACCGGGCGAAGAAGCAGGAAGCGTTGCGGAGAGTTACAGAGAACTTGGCCAGTCTTACTC CCAAAGGCTTGAGCCCAGCCATGTCCCCAGCCCTACAGCGCCTCGTAAGCAGGACAGCCAGCAAGTACAC AGATCGTGCCCTGCGGGCCAGCTATACACCATCCCCAGCACGATCTTCCCACCTCAAGACCCCAGCTGGT GGGCCGCAGACACCCACAAGCACTCCAGCCCCTGGGTCTGCTACACGCACACCCCTCACACAGGACCCAG CCTCCATCACAGACAACCTGCTGCAGCTCCCTGCCCGACGCAAAGCTTCAGACTTCTTTTAGAGCCAGGC CTGACTGGACCTGTGGATGTTTTGTGGAACACTTTGTGGAGCATCTAGGGCAGCTTTCTACCACTCAGAG GATTCCAGGCCTTGGAGACACAGACCACAGTTGATTATTCCAGGAGCCACAAATATCTAGTGTTGTTCCC GTGGCCAGGTTGGCACATTACGCGGACCAGTCCCTTGGAAGTACAGCGTTGGTGCTGTTCCCAGGGCCCT GCTGAAATGCCTTCTAGCTAACCCCCAATTGAATCCTCATTAAAGAACACCTGGAACTGTCTGGCCAGGC TGCTGATCCATGTCCTGGGGCCCACGTGGGTGTCTCAGGGTTGGAAGCAAGGGGTGCTGGGGAGCTGCCT TCTATGCTGAGATCACCCCCCGAGCCTCCCACTGTAAAGGATACCACAGACCCCAAAAGTGGAGCCATTG AGCTTGTGGGCCTCCTAGTCAGAGCTGGCCATTCGGTTTGTTGGTTCTGGGCTTTCGTATCAGGTGTCTT CATATCAGGCTCACCAGGACCAGTTCTCTTCCTGTGACTCGAACCAGTACCTCCCAGCAACCTGGCTGCC TCCCTGTCCTCCTCTCTGCTTGACCCACTGTTCTTCAGCTCTGCCTGCTGGACTCTGCTGAGACACCAGC TTTACCTCAGCCTGAGCTGTACTATGAAAAGAAAGAGTGCAGGCTCCCTGCAAACATGCAAATGCCATGC GCACCAGCAACTAAGAGTAACTAGTCCTCAGGTGACTAGAGGCCAGCTACGCCTGTTAGCCTGTGCCTAC AGAGAGCTGAAGGGTCCCTTCTCGGCCTAACAGAGTCAAAAACTATGACCTAATTCCCCAGCCTACGTAC GGTTTTCAGGCCACAAGTGGCATCCTATGGCCGGCCCTATGTTAAGGGCAGCCTTAAGCACACCAGACTT TTCTGACACCTGGGTCTTGCTTTGATTGCATGAGAGCCACCTTTTCTAGTCTGCTGGCATTGGGCCTCTG AGGCGGGGTTGGGTACCTTCCTCCTTGGGCCTCATACTACTTAGTTTTTGTCAGTTTGACAGAAGCTAGA ATCACCTGGGAAGAAGGAACCTCAATTGAATTACCTCTCATCAGATGGGCTTGACGGCATGTCTGCAGGC ATTTTCTTGATCGTCAATCGATGTAGGAGGGCAGTGGTCTTGGGTTGAGTCAGAAAGCCTGGGAGGAAAC TAGAGAGGAAGCCAGCCAGGACGTGACACTCCTTCATGGTGTCTGCTTCAGCTCCTACTTGAGGTCCTGC TCTGGCTTCCCTCAGTGATGGACTGTGATGGAAAGTGTGAGCTGAATAAACCCATTTCCTCCCTTAAAAA AAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_001081633
Insert Size 1440 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC013711, AAH13711
RefSeq Size 2813 bp
RefSeq ORF 1440 bp
Locus ID 27886
UniProt ID O70279
Cytogenetics 16 11.09 cM
Summary The human ortholog of this gene is located within the minimal DGS critical region (MDGCR) thought to contain the gene(s) responsible for a group of developmental disorders. These disorders include DiGeorge syndrome, velocardiofacial syndrome, conotruncal anomaly face syndrome, and some familial or sporadic conotruncal cardiac defects which have been associated with microdeletion of human chromosome band 22q11.2. The encoded protein localizes to the nucleus, and the orthologous protein in humans co-purifies with C complex spliceosomes. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. The resulting protein (isoform 2) is one aa shorter than isoform 1.
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Documents
Resources
"NM_001081633" in other vectors (4)
SKU Description Size Price
MG207680 Dgcr14 (tGFP-tagged) - Mouse expressed sequence 2 embryonic lethal (Es2el), transcript variant 2 10 ug
$657.00
MR207680 Dgcr14 (Myc-DDK-tagged) - Mouse DiGeorge syndrome critical region gene 14 (Dgcr14), transcript variant 2 10 ug
$289.00 $457.00
MR207680L3 Lenti ORF clone of Dgcr14 (Myc-DDK-tagged) - Mouse DiGeorge syndrome critical region gene 14 (Dgcr14), transcript variant 2 10 ug
$757.00
MR207680L4 Lenti ORF clone of Dgcr14 (mGFP-tagged) - Mouse DiGeorge syndrome critical region gene 14 (Dgcr14), transcript variant 2 10 ug
$757.00

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