Kcnn4 (NM_008433) Mouse Untagged Clone

SKU MC200643

Kcnn4 (untagged) - Mouse potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (Kcnn4), transcript variant 1, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Kcnn4
• Synonyms: IK1; IKCA1; KCa3.1; KCA4; mIKCa1; SK4; SKCas
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010274 sequence for NM_008433; GCAAAAGTCCCAGAGTGAGCAGAACAGGCCGCTGGCCTGGAAGCCAAGCTGGCTGCCATTTGCGTACTGA GAGGTGGGGGACCCTGGGCAGGAAGCTGGCTGAGCCCCAAGACCTCAGGGGCCATGGGCGGGGAGCTGGT GACTGGCCTGGGGGCCCTGAGACGGAGAAAGCGCCTGCTGGAGCAGGAGAAGAGGGTGGCCGGCTGGGCG TTGGTGCTGGCGGGAACTGGCATCGGACTCATGGTTCTGCACGCTGAGATGTTGTGGTTCCTGGGCTGCA AGTGGGTGCTGTACCTGCTCCTGGTTAAGTGTTTGATCACCCTGTCCACTGCCTTCCTCCTTTGTCTTAT TGTGGTCTTCCATGCCAAGGAGGTCCAGCTGTTCATGACTGACAACGGGCTCCGGGACTGGCGCGTGGCG CTGACCCGGCGGCAGGTGGCGCAGATCCTGCTGGAGCTGTTGGTGTGCGGGGTGCACCCGGTGCCCCTAC GGAGCCCGCACTGCGCCCTGGCGGGGGAGGCCACCGACGCGCAGCCCTGGCCGGGTTTCCTGGGCGAAGG CGAGGCGTTGCTGTCCCTGGCCATGCTCCTGCGTCTCTACCTGGTGCCCCGCGCGGTGCTGCTGCGCAGC GGGGTCCTGCTCAACGCGTCCTACCGCAGCATCGGGGCGCTCAACCAAGTCCGCTTCCGCCACTGGTTCG TGGCCAAGCTGTACATGAACACGCACCCGGGTCGCCTGCTGCTGGGCCTCACGCTGGGTCTCTGGCTCAC CACAGCTTGGGTGCTGTCTGTGGCTGAGAGGCAGGCTGTCAATGCCACGGGGCACCTCACAGACACACTG TGGCTGATTCCGATCACATTCCTGACCATTGGCTATGGGGACGTGGTACCTGGCACCATGTGGGGCAAGA TTGTCTGCCTGTGCACCGGAGTCATGGGGGTCTGCTGCACAGCTCTCCTGGTGGCTGTGGTGGCTCGGAA GCTGGAGTTCAACAAGGCGGAGAAACACGTGCACAACTTCATGATGGACATCCATTATGCCAAAGAGATG AAGGAGTCAGCGGCGCGGCTGCTGCAGGAAGCCTGGATGTACTACAAGCACACTCGAAGGAAGGACTCCC GGGCTGCCCGCAGACATCAGCGCAAGATGCTGGCCGCCATCCACACGTTCCGCCAGGTACGGCTGAAACA CCGGAAGCTCCGGGAACAAGTGAATTCCATGGTGGACATCTCCAAGATGCACATGATCCTGTGCGACCTG CAGCTGGGTCTCAGCTCCTCGCACCGTGCCCTGGAGAAGAGAATCGACGGTCTGGCAGGAAAGCTGGATG CCCTGACAGAGCTGCTCGGCACTGCCCTGCAGCAACAGCAGCTACCAGAACCCAGCCAGGAGGCCACATA GCTCCACATGGACTCACAGAAGAACCAGGCTAAGTACCCAAGGACCGAGCTCAAGGACATGCTCCCTGCC AATTCCGACCAAGCCCCACGATAAATCACCTCAAGATGCCAGGACCCACGTGGATCCACGTTGAGGTGCA TGGACTCTGGTGGTGGCAGCCATGAGTGGACACTGAATTGGACCTCCCACGTCAATGGGAACATGGCCCC ACTACACATATGCCCTCATCAGAAGCCCTTTCTGCTGTGCTGCTGTTCAGGACCCCTTAATCTCTGTTGG AGGAGGAGGTGCCTGGGGTCTAGGATGCTAGGAAGCTTCAGTTAACCGCGGGCGGGTCTCGCTGAAGGAA CCAGGTCCGAGCGACGGGAGGTGCTGCCCCCTGGTGGACACCTGGGAAGATGCCGTTTCCTCCTGATTGC AGAGACTGTGCCAGAAGAGGGTACAGGGTGCTGCTCACCAATCCACCCTCATACTTTGTAATAAATGGTA AACAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008433
• Insert Size: 1278 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC010274, AAH10274
• RefSeq Size: 1921 bp
• RefSeq ORF: 1278 bp
• Locus ID: 16534
• UniProt ID: O89109
• Cytogenetics: 7 A3
• Summary: Forms a voltage-independent potassium channel that is activated by intracellular calcium (PubMed:9705284). Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells (PubMed:20884616). Plays a role in the late stages of EGF-induced macropinocytosis (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) and variant 2 encode the same protein.