Spi1 (NM_011355) Mouse Untagged Clone

SKU MC200585

Sfpi1 (untagged) - Mouse SFFV proviral integration 1 (Sfpi1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Spi1
• Synonyms: Dis-1; Dis1; PU.1; Sfpi-1; Sfpi1; Spi-1; Tcfpu1; Tfpu.1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003815 sequence for NM_011355; CTGGTCCTGAGGGGGATCCGCCTTGATCCCCACCGAAGCAGGGGATCTGACCAACCTGGAGCTCAGCTGG ATGTTACAGGCGTGCAAAATGGAAGGGTTTTCCCTCACCGCCCCTCCATCGGATGACTTGGTTACTTACG ATTCAGAGCTATACCAACGTCCAATGCATGACTACTACTCCTTCGTGGGCAGCGATGGAGAAAGCCATAG CGATCACTACTGGGATTTCTCCGCACACCATGTCCACAACAACGAGTTTGAGAACTTCCCTGAGAACCAC TTCACAGAGCTGCAGAGTGTGCAGCCCCCGCAGCTACAGCAGCTCTATCGCCACATGGAGCTGGAACAGA TGCACGTCCTCGATACTCCCATGGTGCCACCCCACACCGGCCTCAGTCACCAGGTTTCCTACATGCCCCG GATGTGCTTCCCTTATCAAACCTTGTCCCCAGCCCACCAGCAGAGCTCAGATGAGGAGGAGGGTGAGAGG CAGAGCCCTCCCCTGGAGGTGTCTGATGGAGAAGCTGATGGCTTGGAGCCTGGGCCAGGTCTTCTGCACG GGGAGACAGGCAGCAAGAAAAAGATTCGCCTGTACCAGTTCCTGCTGGACCTGCTGCGCAGCGGCGACAT GAAGGACAGCATCTGGTGGGTGGACAAGGACAAAGGTACCTTCCAGTTCTCGTCCAAGCACAAGGAGGCG CTGGCGCACCGCTGGGGCATCCAGAAGGGCAACCGCAAGAAGATGACCTACCAGAAGATGGCGCGCGCGC TGCGCAACTACGGCAAGACAGGCGAGGTGAAGAAAGTCAAGAAGAAGCTCACCTACCAGTTCAGCGGCGA GGTGCTGGGCCGTGGGGGCCTGGCCGAGCGGCGCCTCCCGCCCCACTGATCGCCCGCAGAGACCGCCAGG CTCCTGGACCCCGCCGGCCATAGCATTAACCCGTCGCCCGGCCCGGACACAGGGAGGACATTCCCAGGGC CGAGGCAGGACTGGGGGCCCGGCCTCGCCCTCCCATGCCCGGCCTGGCCCGCCCCACCCGCTTTGCCTCC CACCAGGACTCTAGCCCGCTCCAAGGGCCGCCTGGGCCTCGGACCTCAACCGAGGGTCAGCCTGGCTTAG TGGCCACGGTGCTTCCTTGGGAGTCTGGCGCTGGCACCTTTTTGTATATTGAATGCTTTTTAAAAAGCTC TTCCTCCCCACCCCCTCATTAGTCACTAAAGACAAGTAAAATTATTGACAGCTATTCTCCCAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011355
• Insert Size: 819 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC003815, AAH03815
• RefSeq Size: 1286 bp
• RefSeq ORF: 819 bp
• Locus ID: 20375
• UniProt ID: P17433
• Cytogenetics: 2 50.44 cM
• Summary: Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]