Nmnat3 (NM_144533) Mouse Untagged Clone

SKU MC200575

Nmnat3 (untagged) - Mouse nicotinamide nucleotide adenylyltransferase 3 (Nmnat3), nuclear gene encoding mitochondrial protein, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Nmnat3
• Synonyms: 4933408N02Rik; PNAT3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC005737 sequence for NM_144533; TGGGAGCGCAAGGTCAAGTTCACTCGCGGCTCCGCCCCCAGCCTGGGTCAAGACTAGTTAGTCTTAGGGC GATCTGCGGGGTGACCCATCCTGGTCGAGCAGCAGATCCGCGGTCCCTGTTGACTCTGAGCGGAGCCCCG CGACGCGCAGGTAAGCACAGCCAGCGACCTTGGCTGCCTGCTTCGCCCGCGTGGGTGTCCACGAAGCCTT GAGTCCCCGCGTACCTACCAGGCCCACGTGACTCTCGGGGGCGGCGACTCGGGCGCGCGCGCGCACGACA GAGTCAGATGGCTGTGGAGCGCAGAACCACGCTAGCCCCACGGTCACTTTTCTGACCGATTGGCTCGAGC TGACACGGGTTCGGATCCCCCCTGCAGCACGTTTACAGTCAGTTAAAACAGGGTGGCCACTGCGGGTGTG GAGGTGTGTGACAGCATGAAGAACCGAATCCCTGTGGTGCTTCTGGCCTGTGGTTCCTTCAACCCCATCA CGAATATGCACCTGCGCTTGTTTGAGGTGGCCAGAGACCACCTACACCAAACAGGAAGGTACCAGGTGAT TGAGGGCATCATCTCACCCGTCAATGACAGCTATGGGAAGAAAGACCTGGTGGCTTCCCATCACCGAGTG GCCATGGCCCGGCTGGCCCTGCAGACATCTGACTGGATTCGGGTGGACCCCTGGGAGAGTGAGCAGGCGC AGTGGATGGAAACGGTGAAGGTGCTGAGGCACCATCACAGGGAGCTGCTCAGATCCTCAGCCCAGATGGA TGGCCCAGACCCCAGCAAGACACCATCAGCCTCTGCAGCACTGCCAGAGTTGAAACTCCTCTGCGGAGCT GATGTCCTCAAGACCTTCCAGACCCCCAACCTCTGGAAAGACACGCACATCCAGGAAATAGTGGAGAAGT TCGGCTTGGTGTGCGTGAGCAGGAGCGGTCATGACCCGGAAAGGTACATCTCGGACTCGCCCATCCTCCA GCAGTTTCAGCACAACATTCACCTGGCCAGGGAACCCGTTCTGAACGAGATCAGTGCCACATACGTCAGG AAAGCCTTGGGACAAGGGCAGAGCGTGAAGTACCTCCTCCCTGAGGCCGTCATCACCTACATCAGGGACC AGGGCCTCTACATCAATGACGGTTCCTGGAAAGGGAAAGGAAAGACTGGCTAGAGGGCCTCCTGGGGAGT AGGGGTATTCTGCTCTGGGTTCTGGGTTTGTTTCCACAGTGGTTTTTTTTTACCATGGGTGACTTCCTAC TCCAGGAAGAGATCGTGCCTGTGGCAGAGACAGTCGAGTATCAGAGCAACTTAAGAGGCATGACAGGTCA TAAGATTAGGGTCACTAAGGAGGAGACCTGCTTACTGTGTAAATGGCAGTCATGGGAGGCACACTGATGC TGTCCTGAGCATGGTTGGCCCCTGAGGGGTCCATCAAGTCGCCTTCGTTCGGGTTTTGTTTTTGTTTTTT GTTTTATAACTCGGGCCAGGTGCAGCATGCTGGTCTTGATAGGCAGAGTTTGACAGGGCGCTAATTACTG AGCAGAGGACCTTTTCAACATCCTCGGCTCAAACAATAATAGCAAGTGAGGATTCAGAAATCTGGAAACA GAACATCATTGATTTCCAATGTATCCTCCCTTGTAACCATGGCCTGCCTGCCTGTCTGTCTGTCTGCCAC CTGCCTGTGGTGTAACATAAAATTTTTGACTACCTTCTTTCTATGTTGTGGTCAAGCAATATGTGTTTAA GATCTGTTTTATCATATGATCGTATGTGTGTGTGTGCATGTGTCTTTACATGTGTGTGTACATGCACATG GGAGGGACATCAGAGTGCCTGAAACCCAAGTTACTGGCAGTTGTGAGCCCCCTGATGTGGGTGCTGGAAC CCAAACTCCAGTCTTAGGGAAGAGCAGCAAGAGAGTTCTTAACCACTAAGCTGTCTCTCCAGCCCCCTCA AGCAATATTTTTGATACACCATGTATTTTTACACTGCTATACTCTAAGCACCAGGGTTCCCAATATCCTG ATTTCTAAAAGGGAACCGAAGTAAAACAGATGCCTGACTGTTTTAGAAGATCTTTTGAATGTTTTATGAC TGCCTGCTTGTTTGAATATTGGCAAAAAGATAAATAATAAATTGACATCAAAAATACTAAAAAAAAAAAA AAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_144533
• Insert Size: 738 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC005737, AAH05737
• RefSeq Size: 2173 bp
• RefSeq ORF: 738 bp
• Locus ID: 74080
• UniProt ID: Q99JR6
• Cytogenetics: 9 E3.3
• Summary: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD(+), NADH, NaAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following injury.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.