Bid (NM_007544) Mouse Untagged Clone

SKU MC200325

Bid (untagged) - Mouse BH3 interacting domain death agonist (Bid), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Bid
• Synonyms: 2700049M22Rik; AI875481; AU022477
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC002031 sequence for NM_007544; GGAGTAGAGCGCCGGGCCGAGTGTGGCTCCGCAAACCTTTGCCTTAGCCCGTTCGCCGCCCGGTACCGGC GCAGCGGCGTCTGCGTGGTGATTCCCTGGACTCTGTGAGCTGGCAGTGCTTGGAGCTACACAGCTTGTGC CATGGACTCTGAGGTCAGCAACGGTTCCGGCCTGGGGGCCGAGCACATCACAGACCTGCTGGTGTTCGGC TTTCTCCAAAGCTCTGGCTGTACTCGCCAAGAGCTGGAGGTGCTGGGTCGGGAACTGCCTGTGCAAGCTT ACTGGGAGGCAGACCTCGAAGACGAGCTGCAGACAGATGGCAGCCAGGCCAGCCGCTCCTTCAACCAAGG AAGAATAGAGCCAGATTCTGAAAGTCAGGAAGAAATCATCCACAACATTGCCAGACATCTCGCCCAAATA GGCGATGAGATGGACCACAACATCCAGCCCACACTGGTGAGACAGCTAGCCGCACAGTTCATGAATGGCA GCCTGTCGGAGGAAGACAAAAGGAACTGCCTGGCCAAAGCCCTTGATGAGGTGAAGACAGCCTTCCCCAG AGACATGGAGAACGACAAGGCCATGCTGATAATGACCATGCTGTTGGCCAAAAAAGTGGCCAGTCACGCA CCATCTTTGCTCCGTGATGTCTTCCACACGACTGTCAACTTTATTAACCAGAACCTATTCTCCTATGTGA GGAACTTGGTTAGAAACGAGATGGACTGAGGAGCCCGCACAAGCCCGATGGTGACACTGCCTCCAGAGGA ACCGCGACCATGGAAAGACCTTGGCCTGAAGACAGGTCCCAGAGAACAGCTGTCTCCCTATTTCCAGGTG GTGGGAACCCCAAGCTGGTGATTCACTGGACATCTCTGCGTTCAGCTTGAGTGTATCTGAAGAGTTTACG CCGGCTCCTGCATCCACACCATGTACCTTTGTCCTATCAGCTGTATGGGTTCCCACTTGGGAATGAAACT TAACAGCAGGCTGTAAGGCAGAAAAGCATCTTTGTAATGCCAAGTGACTGTTCCTGAGAGCCAGCTCTGG GCTGTCTTCACCATGTAGGTGGGCTTCTGTCTAAGGAGAACAGCATTAGGAGAGGTGCATCGGCCCATGA GCGTGAAGTCCACCCAGCCTAGTGGACACTGAAGTGCTCACAAGGCCTCCACCTGCCTTTGTAAAAGCCG AATGGCTGATCTCAAACCATGGGAAGCCCGACCGCCCCACCCCTCCTCACCCCAGCGTTTAGCTGTTTCA GGGGTCAGCTATTATCTCAAGATTTCTATCCAAGTGGAAACAAACTGAATCATGCACACGACTTATCTGT GTGGTGTCAGTTACACTCAGGCTCTTGCTACGGAATGCAAAGAACAACTCACATACCAGTGTCAAACAGA ATGCACAGAAGAGACCTAAAACAGCAGCAGGTCACTCGGTTCACAAAAGGTGACTCCCAGTCAAGTCTGA CACACTGTCTTGGTTGTAGAGCACAGCTGCCATCCTCTTTCCCTGGGTAACATCACAGAAGATTCCATAT CAAAAGCAAATGTTCCCTCCGCTTCTGTATTTCAGAGACAAGGCCTCACTGTATCCTCAAGCGTTGTTAC GTCTTGTGCTGAACTTTGCTTAAAGCTGGGATCGTCAGCACGAGCCGCCACAGCCTACAAGTATTCTAGT TCTGAACTCATCCCAGCCATGGTGGCTGTGATGGCTTGGGTGTATCATACCTGTAAATTAGTGGATTTTT CTTTAGGAACATGACCTTTGGGTGAGTATAATTGAGAAATTATTTTAATTCAGAAAGTACTTTTCATTGT GTTCTAAAAATATGTGAATTGTCTTAAGTGGTAGAAATTTGTTTCTTCAAAATAAAAGGCTCTTCTCTAG AAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007544
• Insert Size: 588 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC002031, AAH02031
• RefSeq Size: 1906 bp
• RefSeq ORF: 588 bp
• Locus ID: 12122
• UniProt ID: P70444
• Cytogenetics: 6 57.02 cM
• Summary: Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c.[UniProtKB/Swiss-Prot Function]