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Becn1 (NM_019584) Mouse Untagged Clone

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SKU
MC200283
Becn1 (untagged) - Mouse beclin 1, autophagy related (Becn1), (10ug)
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ORF Plasmid:
Tag Free DDK
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Becn1
Synonyms 4921513J16Rik; 5430417M23Rik; Atg6
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC005770 sequence for NM_019584
GGGAAGTAGCTGAAGACCGGGCGATGGGAACTCTGGAGGTCTCGCTCCGGGGCGGACCCGAGGCCACCGT GCCCCCGCGGTAGAACGAGCCTGGGTGGCGGGCTCCGGGCTCCTGAGGCATGGAGGGGTCTAAGGCGTCC AGCAGCACCATGCAGGTGAGCTTCGTGTGCCAGCGCTGTAGCCAGCCTCTGAAACTGGACACGAGCTTCA AGATCCTGGACCGGGTCACCATCCAGGAACTCACAGCTCCATTACTTACCACAGCCCAGGCGAAACCAGG AGAGACCCAGGAGGAAGAGGCTAACTCAGGAGAGGAGCCATTTATTGAAACTCGCCAGGATGGTGTCTCT CGAAGATTCATCCCCCCAGCCAGGATGATGTCTACAGAAAGTGCTAATAGCTTCACTCTGATCGGGGAGG CATCTGATGGTGGCACCATGGAGAACCTCAGCCGGAGACTCAAGGTCACTGGAGACCTGTTTGACATCAT GTCGGGCCAGACCGATGTGGATCACCCGCTGTGTGAGGAATGCACAGACACTCTTTTAGACCAGCTGGAC ACTCAGCTCAATGTCACTGAGAATGAATGTCAGAACTACAAACGCTGTTTGGAGATCCTAGAGCAGATGA ATGAGGATGACAGTGAGCAGCTGCAGAGGGAGCTGAAGGAGCTGGCCTTGGAGGAGGAGAGGCTGATCCA GGAGCTGGAAGATGTGGAAAAGAACCGCAAGGTGGTGGCAGAGAACCTGGAGAAGGTCCAGGCTGAGGCG GAGAGATTGGACCAGGAGGAAGCTCAGTACCAGCGGGAGTATAGTGAGTTTAAAAGGCAGCAGCTGGAGT TGGATGACGAACTCAAGAGTGTGGAGAACCAGGTGCGCTACGCCCAGATCCAGCTGGACAAGCTCAAGAA AACCAATGTCTTCAATGCCACCTTCCACATCTGGCACAGCGGACAGTTTGGCACAATCAATAATTTCAGA CTGGGTCGCTTGCCCAGTGTTCCTGTGGAGTGGAATGAAATCAATGCTGCCTGGGGCCAGACAGTGCTGC TGCTCCATGCTTTGGCCAATAAGATGGGTCTGAAGTTTCAGAGGTACCGACTTGTTCCCTATGGAAATCA TTCCTATCTGGAGTCTCTGACAGACAAATCTAAGGAGTTGCCGTTATACTGTTCTGGGGGTTTGCGGTTT TTCTGGGACAACAAGTTTGACCATGCAATGGTAGCTTTTCTGGACTGTGTGCAGCAGTTCAAAGAAGAGG TGGAAAAAGGAGAGACTCGATTTTGTCTTCCGTACAGGATGGACGTGGAGAAAGGCAAGATTGAAGACAC TGGAGGCAGTGGCGGCTCCTATTCCATCAAAACCCAGTTTAACTCGGAGGAGCAGTGGACAAAAGCGCTC AAGTTCATGCTGACCAATCTCAAGTGGGGTCTTGCCTGGGTGTCCTCACAGTTCTATAACAAGTGACTTG CTCCTTAGGGGATGTTTGCCTTTAAGGTTTTATACTTTGTTTGGTTTGGAAAGATGCTTTAAATTAAATT TGGGTAATATTAAACCACATGTTTACAATACCAAAATCCACAAAAGCTACTTTATTTTCAAATATGACAG ATAGTTTCCAGAGTACGCCATGTATAGCAAAGAACCCTGCCATAGTTTTGACTCAGCCCCATGCATCCTT TCCCTCTTTCCTGAAAACAACTAATTTAAATTTGCTTTGTTTTCTTTTTTAAGTTGAATTGACGTTAATG TGTTTTCACTGGATTTTATCTCTCTCAACTTCCTGCACTTAAAATTTGAAACAGCAAAGGTTTGAGATGA GATGCTTGTGGCACACAGTTGGGTGATGTGGGGAAAGGACACCGGGTCAGGAGTTGCAAGTTTAACTCCG TCCTCACTTGTAGCATTGAATGCCTCCTGTGCTGTCTAGTGGGACTACAGAATGCTGTTTGATACTGTGT GCGACGTGGAGAGATTTAATTATTTGTAATAAAGGATTTGCTATGGTCTATTAAAAAAAAAAAAAAAAAA AAAA
Restriction Sites RsrII-NotI |
ACCN NM_019584
Insert Size 1347 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC005770, AAH05770
RefSeq Size 2034 bp
RefSeq ORF 1347 bp
Locus ID 56208
UniProt ID O88597
Cytogenetics 11 D
Summary Plays a central role in autophagy (PubMed:10604474, PubMed:12372286, PubMed:19270693, PubMed:28445460). Acts as core subunit of different PI3K complex forms that mediate formation of phosphatidylinositol 3-phosphate and are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed:19270693, PubMed:25275521). Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (By similarity). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms (PubMed:25275521). Involved in endocytosis including endosome formation in neuronal cells (PubMed:25275521). May play a role in antiviral host defense (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
Resources
"NM_019584" in other vectors (3)
SKU Description Size Price
MR207162 Becn1 (Myc-DDK-tagged) - Mouse beclin 1, autophagy related (Becn1) 10 ug
$686.00
MR207162L3 Lenti ORF clone of Becn1 (Myc-DDK-tagged) - Mouse beclin 1, autophagy related (Becn1) 10 ug
$986.00
MR207162L4 Lenti ORF clone of Becn1 (mGFP-tagged) - Mouse beclin 1, autophagy related (Becn1) 10 ug
$986.00

file-alt Citations

Autophagic program is regulated by miR-325
Bo, Su-Ling, Fang et al
Cell Death Differ. (2014)
Cited by 43
AI Summary: In this study, OriGene's mouse E2F1 cDNA and Beclin 1 products were used to investigate the molecular mechanisms by which E2F1 and autophagy-related genes regulate the autophagic program in cardiomyocytes.
Snippet: Cells culture, anoxia/reoxygenation, apoptosis and cell death assay. Details are in the Supplementary Methods. Adenoviral constructions and infection. Mouse E2F1 cDNA and Beclin 1 were from Origene. The GPF-LC3 expression plasmid was from Cell Biolabs Inc ... Cite SaveSaving
Showing 1-1 of 1 papers.
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