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Prr14 (BC006909) Mouse Untagged Clone

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SKU
MC200258
Prr14 (untagged) - Mouse proline rich 14 (cDNA clone MGC:6888 IMAGE:2651775), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Prr14
Synonyms MGC6888
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC006909
AAAAAACAAAAAAAAACAAAAAAACCCACCAAAAGGGAAATGGAAGTATAAATCAATTTTTGTATAATCT GTTTTTGATATTCAGAAAGTGCAGATTTAACAAAGGTAGCCTTATCCTTTCTCTGTACAATGTCGATTAT AATTATGCAAACAACAAAAGTCCGATCAAAGCTTGAAAGCTTTGCTGATATCTTCTTCACACCTAACAAA ACACCACAGCCTCCACCCCCTTCACCTCCCATGAAATTAGAATTGAAGATTGCCATCTCGGAGGCTGAGC AGTCCAGGGCTACTGAGAAAATTACATCTGTCAGCCCCAGGCCCCCTATCCGCCAGTGGCGGACTCAGTG CAATTCCCTAGCACCTGTCTCTAAGTCATCTCTGGGACGAAGCTACTCCTGCCCTGATCTAGGGCCCCCT GACCCAGGTAGCTGGCCTCCTGTCCCATCCCAGCCAAGCCAGTCCAGGCCCCGGAGACATACTGTGGGTT GTGGGGAGATGGCCCGGACCCCACCACCTCCTCGACCCTGTCTCCGGAAAGAGGTCTTCCCTCTTGGAGG AGTGGGAGTCTCTCCTTCTCTCACTACATCTTGCTCAGCCAATGCCCCTGCTTCCTTCTTCTGCGAGCCG GCAGAACCCAGGTTGGGTTCAACGAAGGGGAAGGAGTTAAGAGCCTCAAAAGACAAGGTGTTTTCTGATC CAGAGACCAAGACCATGGGGAAGGTTTCTAGATTCAGAATACGGAGGACACCTGTCCGTCTCCAACCAAA CCTTACACCAATGGGGCTGCCTCGACCAATCAGGTTGAATAAGAAGGAGTTCACTTTGGAAGAAATTTAT ACAAATAAGAACTACCAATCACCCACAACCAGAAGGACCTTTGAGACCATCTTTGAGGAACCACGGGAGC GCAATGGAACTCTGATTTTTACCAGCTCTAGGAAGCTCCGAAGGGCAGTAGAATTTCGGGACAGTAGCCT TCCTCGATCCCGGCGACCATCACGAGGGGTCCGCACCGCAGCAAGCAGGACCCTTACTCCCAACCTGGCA CCCAGCCAGGATGTGGGGTCTCTACTGCAAGAAAGGCTGAGGGAGCTAGATGCCTTGCTCCTGGAGGAGG AGACAGATAAGGAACACCCTTGTCACCTCTAGGAGCTCCAACTATTTGTCCATCCGTCCTTTAGGTAGGG GAGAAATCTCTGAATCAATTGTATTTTTTTTTCTCTATATTTCTATAAAGTTTTCAATATAAAAAAAAAA AAAAAAAA
Restriction Sites RsrII-NotI |
ACCN BC006909
Insert Size 1023 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC006909
RefSeq Size 1268 bp
RefSeq ORF 1023 bp
Locus ID 233895
Cytogenetics 7 F3
Summary Functions in tethering peripheral heterochromatin to the nuclear lamina during interphase, possibly through the interaction with heterochromatin protein CBX5/HP1 alpha (By similarity). Might play a role in reattaching heterochromatin to the nuclear lamina at mitotic exit (By similarity). Promotes myoblast differentiation during skeletal myogenesis, possibly by stimulating transcription factor MyoD activity via binding to CBX5/HP1 alpha (PubMed:25906157) (By similarity). Involved in the positive regulation of the PI3K-Akt-mTOR signaling pathway and in promoting cell proliferation, possibly via binding to GRB2 (By similarity).UniProtKB/Swiss-Prot Function
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"BC006909" in other vectors (4)
SKU Description Size Price
MG205081 Prr14 (tGFP-tagged) - Mouse proline rich 14 (cDNA clone MGC:6888 IMAGE:2651775) 10 ug
$657.00
MR205081 Prr14 (Myc-DDK-tagged) - Mouse proline rich 14 (cDNA clone MGC:6888 IMAGE:2651775) 10 ug
$289.00 $457.00
MR205081L3 Lenti ORF clone of Prr14 (Myc-DDK-tagged) - Mouse proline rich 14 (cDNA clone MGC:6888 IMAGE:2651775) 10 ug
$757.00
MR205081L4 Lenti ORF clone of Prr14 (mGFP-tagged) - Mouse proline rich 14 (cDNA clone MGC:6888 IMAGE:2651775) 10 ug
$757.00

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