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Ift20 (BC002040) Mouse Untagged Clone

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SKU
MC200244
Ift20 (untagged) - Mouse intraflagellar transport 20 homolog (Chlamydomonas) (cDNA clone MGC:6005 IMAGE:3590790), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ift20
Synonyms RP23-399H5.8
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC002040
TGCTTCTGGACTCGGGGGTGACGTGTGGCTCCTAGATCTTGTCTCCGTGGCAGCAGCCGGTTGCAGTTGC CGGAGAGAAGCTTCTACCGACAGTCGTCAGGGCCGCAGAGCGCTGCCGAGGCGGCTCGTCAGCTAAGCTG TGCTGCTCCAGGGCAGGCACCATGTTGCATTCATCTTTACGCCCAGCATCTAGCAGTGTCGGCATGTAGT AGGCACTCAAGAAATGTATGTTGAATGAACGATGTCTGTGACAAGCAACTGGACTTTCCTCTTTCCTGAC CCTTGCTCCTATGACATACCTCCTGATTGCCACTGTCACCCCTTCAAAGCAGACCTCTAGGGCTTCCTGG AAGGGAAACAGCTATGGCCAAGGACATCTTGGGCGAGGCAGGGCTGCATTTTGATGAACTGAACAAGCTC CGAGTGTTGGACCCAGAGGTCACCCAGCAGACTGTGGAGCTCAAGGAGGAGTGCAAGGACTTTGTGGACA AAATTGGCCAGTTTCAGAAAATTGTTGGTGGTCTAATTGAGCTTGTTGATCAGCTTGCCAAAGAAGCAGA GAACGAGAAGATGAAGGCCATTGGTGCTCGGAACTTGCTGAAGTCCATAGCGAAGCAGAGAGAAGCCCAG CAGCAGCAGCTGCAGGCCCTGATAGCAGAAAAGAAGACGCAGCTAGAAAGGTAAGCGGCCATGGCCACTG CAAACGCCTTTTCCTTCTCTGCCACCTTGGCTTCCCTCACCATTTCCCAGGTAGAGCATCTCACTTTAAA AATTGATAGAAGAAAGTGGGGACTTGGTTAATTCCAAATTGCCTCCATGGTGTGAGCACTGGTTCTTATA ACAAGGACTGAGGCCACCTCCCGTGCCTTCCCTGGGGTAGAGTGTTCTCCCAGGTGGCCCTAAACAGGGC TTTGCTTATCTGACACTCTGGCCCTGCCTTCTCAATTCATGGTTTCTTGCATCTGTTTTAAAGCTCTATA CACTCAAAGAATTATGTTTTTATTTCATTCTTTTCAGGGCTTCTAACAATTTTGTTTTTCAGGTATCGGG TTGAATATGAAGCTTTGTGTAAAGTAGAAGCAGAACAAAATGAATTTATTGACCAATTTATTTTTCAGAA ATGAACTGAAAATTTCAGTTTTATAGCAGGAAGGAAAAAAAAAAAAAGCACCCCAACCAAAAAAAAAAAA AAA
Restriction Sites RsrII-NotI |
ACCN BC002040
Insert Size 321 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC002040, AAH02040
RefSeq Size 1193 bp
RefSeq ORF 321 bp
Locus ID 55978
Cytogenetics 11 B5
Summary Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium (PubMed:16775004). Regulates the ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway. Required for protein stability of E3 ubiquitin ligases CBL and CBLB that mediate ubiquitination and internalization of PDGFRA for proper feedback inhibition of PDGFRA signaling (PubMed:29237719). Essential for male fertility. Plays an important role in spermatogenesis, particularly spermiogenesis, when germ cells form flagella. May play a role in the transport of flagellar proteins ODF2 and SPAG16 to build sperm flagella and in the removal of redundant sperm cytoplasm (PubMed:27682589). Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment (PubMed:24089209).UniProtKB/Swiss-Prot Function
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"BC002040" in other vectors (4)
SKU Description Size Price
MG200367 Ift20 (tGFP-tagged) - Mouse intraflagellar transport 20 homolog (Chlamydomonas) (cDNA clone MGC:6005 IMAGE:3590790) 10 ug
$350.00
MR200367 Ift20 (Myc-DDK-tagged) - Mouse intraflagellar transport 20 homolog (Chlamydomonas) (cDNA clone MGC:6005 IMAGE:3590790) 10 ug
$289.00
MR200367L3 Lenti ORF clone of Ift20 (Myc-DDK-tagged) - Mouse intraflagellar transport 20 homolog (Chlamydomonas) (cDNA clone MGC:6005 IMAGE:3590790) 10 ug
$450.00
MR200367L4 Lenti ORF clone of Ift20 (mGFP-tagged) - Mouse intraflagellar transport 20 homolog (Chlamydomonas) (cDNA clone MGC:6005 IMAGE:3590790) 10 ug
$450.00

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