Rfx2 (NM_009056) Mouse Untagged Clone

SKU MC200173

Rfx2 (untagged) - Mouse regulatory factor X, 2 (influences HLA class II expression) (Rfx2), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Rfx2
• Synonyms: 5430432H19Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC004654 sequence for NM_009056; CCCACGCGTCCGGGAGCGGCCGGCGCCGCCACCGCCGCCGCCATAGAGACTGTAGCCGTGGAGACTGTTA CTTACCAACGCGGAGCGACACGCAGCGGCCGACGCCGCGGGAGCCGCTACCGGAACTCCCTGCCGAACTC CAGATCCCAGCATGCAGAACTCCGAGGGAGGAGCAGATTCGCCAGCGTCAGTGGCTCTGCGTCCTGCAGC CCAGCCCATGCCAGCCTCCCCACAGAGGGTGCTGGTCCAAGCTGCAGGCTCCACTCCCAAGGGTACCCCG ATGCAGACACTCACCCTCCCCAGAGTCCAGCCAGTGCCACCACAGGTGCAGCACGTGTACCCCGCGCAGG TGCAGTACGTGGAGGGTGGGGACGCTGTCTACGCCAACGGAGCCATCCGAGCTGCCTACGCCTACAACCC AGACCCTCAGCTTTACGCCCCCAGCAGCGCAGCATCTTACTTTGAGACTCCAGGTGGCACTCAGGTGACG GTGGCAGCCTCATCCCCACCTGCAGTCCCCTCCCACGGCATGGTGGGCATCACCATGGACGTCTCGGGGA CCCCCATTGTGTCTGGCGCAGGAGCCTACCTCATCCATGGGGGCATGGACGGCACAAGACACTCTCTGGC CCACACTGCACGATCCTCACCTGCCACGCTCCAGTGGCTTCTGGACAATTACGAGACAGCGGAGGGTGTG AGCCTGCCCCGCAGCTCCCTGTACAACCACTACCTGCGCCACTGCCAGGAGCACAAGCTGGAGCCAGTCA ATGCCGCATCCTTTGGCAAACTCATCCGCTCTGTGTTCATGGGCCTGCGCACACGACGCCTGGGGACCAG GGGCAACTCAAAGTACCATTACTATGGGATCCGCCTGAAGCCAGACTCCCCACTGAACCGACTGCAGGAA GACACACAGTACATGGCCATGAGGCAGCAACCCACACACCAGAAGCCTAGGTATCGGCCAGCCCAGAAGT CAGACAGCCTCGGGGATGGCAGCGCTCACAGCAACATGCACGGCATGCCCGATCAGGCCATGGCCACCCA GGGCCAGCATCACCAGCAGTACATAGATGTGTCCCACGTCTTCCCAGAGTTCCCGGCACCTGACCTAGGC AGCACGCTACTGCAGGAGAGCGTCACCCTTCATGATGTGAAGGCCCTGCAGCTGGTGTATCGCAGGCACT GTGAGGCTACCTTAGATGTCGTCATGAACCTCCAGTTTCAGTACATTGAGAAGCTCTGGTTGTCCTTCTG GAACTGCAAGGCCACCTCCAGCGATAGCTGTGCCTCGCTGCCTGCCAGCGATGAGGACCCAGAAGTCACC CTCCTCCCTAAGGAGAAGCTCATCTCTCTGTGTAAATGTGAGCCGATCCTACAGTGGATGCGAAGCTGCG ATCACATCCTGTACCAGACGCTGGTGGAGACCCTTATCCCAGATGTGCTGCGGCCTGTCCCCAGTTCCCT CACCCAGGCCATCAGGAACTTCGCCAAGAGCCTAGAGGGCTGGCTCATCAATGCCATGAGTGGCTTCCCT CAGCAGGTCATCCAGACCAAGGTGGGTGTTGTAAGTGCCTTCGCACAGACACTGCGGCGCTACACGTCCC TCAACCACCTGGCGCAGGCGGCACGTGCTGTGTTGCAGAACACGTCACAGATTAACCAGATGCTGAGTGA CCTCAACCGCGTGGACTTTGCCAATGTGCAGGAGCAGGCCTCGTGGGTGTGCCAGTGTGAGGAGAGCCTG GTGCAGCGTTTGGAGCATGACTTCAAGGTCACCCTGCAGCAGCAGAGCTCGCTGGACCAGTGGGCCAGCT GGCTAGACAACGTGGTCACCCAGGTCCTGAAGCAGCACTCAGGCAGCCCCAGCTTCCCCAAGGCTGCGCG CCAGTTCCTGCTCAAGTGGTCCTTCTACAGCTCCATGGTGATCCGTGACCTGACCCTGCGCAGTGCTGCC AGCTTCGGTTCCTTCCACCTCATCCGGCTGCTCTATGACGAGTACATGTTCTACCTGGTGGAGCACCGTG TGGCCCAGGCCACCGGGGAGACGCCCATTGCCGTCATGGGCGAGTTCAACGATCTGGCCTCCCTGTCACT GACCCTGCTGGACAAAGAGGACATTGGGGATGGACACAGCAGTGAGGCAGATGTGGATGGCCGCAGCCTG GGTGAGCCCCTCGTGAAACGGGAACGGAGTGACCCCAGCCACCCACTGCAGGGCATCTAGCCCCTTCCCT GGGCGTCTTCCACGTGCTTCCGGAAGGCGTCACCTGGCCCTCTAGGGACCTGGACTCTCAGCTGTATCAC ACTAGTGCCTCTTCAATGATGCCATGTTTACTGTGACCCAAGGTGGCATGGGGTCCCCACCCACAGGGTG CCAGAGACTGTGGGCAACTAAGATGGTCACTCAAGCTGCCATACTCTGCTAAAGTGGCCCTGACCCTGGG CTGTCCCCCAAAGGATACAGCAGCCTGCTGTGTGTATGTGAGTTGTGGGGCACATCCCCAGAAATGTCCC AACCACCTCGGTGGCTTTCCCACTCAGCTAACCACCATGTCAAAGAAAAACCACTCCAACAGAGCCCAGA GGGGTAGGAGCCTACTATTCTCTGGCCATTGTGTGGAGGCTCTGCAGGTCCCCTGCGCTGTAGTCTCCAT GCATCTAATGGCCTCTCATGGACAGCCAAGAACTCCAGGCACACACCCTGGATACATGCTAACGGCATCT CATCTCATCCTGGACAGGTCCAAGCCCCAAAGAGATAACAGGCTCCAAGCAGGGCCAGTCAAGCCCAGAT GATCGACCCATCTGCTTGACTATAGACTTTCTGTCCAATTCATGGTTAATACCAAGATAACCTGGGGGGC CTGTGTTAGGGTTCCCCAAGTAGCCACAGGGGTCAGCACCATATCCACGCCTCCCAACAAAACTCCAAAA CCTGTGCTCAGGGCAGTGCCTGAGCCCCTGCAGTCTTCCAGACACCTCCCCCAAACTAGCCATGCTTGCT GCTGGCCAGTGGCCAACCCCTTCTCAACCCTAGACCATGAGATCTCTGCAGACAAATGAACACCAATGCC TATTGGGCCCTGAGTTCTAGAGGCCCTAACATGTTACACCTTGCTGCTGTCGTCATGTTGAGAATTCTTT CCCAACACAAGCCCTGCCCTCCCACACCTAGGAAGCGCCTAATGAAATATGATAGTTTTTTTTTTTTTGA AAAACTGGGGCCCCCAATAGTTCTGTGCTGTAATTGTTCTTAGAAGGTTCTTATGAATGTGTCCATCGCA ACATAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_009056
• Insert Size: 2079 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC004654, AAH04654
• RefSeq Size: 3309 bp
• RefSeq ORF: 2079 bp
• Locus ID: 19725
• UniProt ID: P48379
• Cytogenetics: 17 29.5 cM
• Summary: Transcription factor that acts as a key regulator of spermatogenesis (PubMed:26248850, PubMed:26162102, PubMed:26853561). Acts by regulating expression of genes required for the haploid phase during spermiogenesis, such as genes required for cilium assembly and function (PubMed:26162102, PubMed:26853561). Recognizes and binds the X-box, a regulatory motif with DNA sequence 5'-GTNRCC(0-3N)RGYAAC-3' present on promoters (PubMed:15229132, PubMed:26162102). Probably activates transcription of the testis-specific histone gene HIST1H1T (PubMed:15229132).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.