Dctn2 (NM_027151) Mouse Untagged Clone

SKU MC200162

Dctn2 (untagged) - Mouse dynactin 2 (Dctn2), transcript variant 3, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Dctn2
• Synonyms: 2310042E05Rik; C87049; C130077D06Rik; DCTN-50; GMP23-48K; p50; RBP50
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC004613 sequence for NM_027151; GTCGCCTTTGCTGGGGACCCGGACCCAGCCTCTCCCCCACTCGGTCACCAGCCCCGGCTCCTCCGAGGCC TCGGTCCCCCAGCCCCGCCTCGCCGCCGCCATGGCGGACCCTAAATACGCCGATCTCCCCGGCATTGCCA GGAATGAGCCAGACGTTTATGAAACCAGCGACCTACCTGAGGATGATCAAGCAGAGTTTGATGCGGAGGA ACTGAGCAGCACAAGCGTGGAACACATCATTGTCAACCCCAACGCTGCCTACGACAAGTTCAAGGACAAG AGAGTGGGAACAAAGGGGCTTGATTTCTCAGATCGCATTGGAAAGACCAAGAGGACAGGATATGAGTCTG GAGACTATGAAATGCTTGGAGAGGGTCTGGGAGTGAAGGAGACACCGCAGCAGAAGTACCAACGACTACT GCATGAAGTCCAAGAGCTGACAACTGAGGTGGAGAAGATCAAGACGACAGTGAAGGAGTCAGCCACCGAG GAGAAGCTCACCCCTGTGGTGCTGGCTAAGCAGCTGGCTGCCCTGAAGCAGCAGCTGGTGGCTTCCCACC TGGAGAAGCTTCTGGGACCAGATGCTGCAATCAACCTTGCCGACCCAGACGGAGCCCTAGCTAAGCGCTT ACTGCTGCAGCTGGAAGCAACTAAGAGCAGCAAAGGCAGTTCAGGGGGTAAGGCTACAGCTGGGGCCCCC CCAGACAGCAGCCTGGTCACCTATGAACTCCACTCACGGCCTGAGCAGGACAAGTTCTCTCAAGCTGCCA AAGTTGCAGAACTCGAGAAGCGTCTGACAGAGCTGGAGGCCACTGTCCGATGTGACCAGGATGCTCAGAA TCCCCTTTCTGCAGGTCTACAGGGAGCCTGCCTTATGGAAACGGTAGAACTGTTGCAAGCCAAAGTGAGT GCCCTGGACCTTGCAGTTCTGGACCAAGTAGAGGCTCGGCTTCAGAGTGTCCTGGGAAAGGTGAATGAGA TTGCTAAGCACAAGGCATCCGTGGAGGACGCAGACACTCAGAACAAGGTGCACCAGCTCTATGAGACCAT CCAGCGCTGGAGCCCAGTCGCCTCCACCCTCCCTGAACTGGTACAGCGACTTGTCACCATCAAGCAGCTG CATGAGCAAGCTATGCAGTTTGGTCAGCTCCTGACACACTTGGATACCACCCAGCAGATGATGGCCAGTT CCCTTAAGGATAATACTGCTCTCCTGACCCAGGTGCAGACAACGATGCGTGAAAACTTGGCCACAGTTGA GGGGAACTTTGCCAGCATTGATGCTCGGATGAAGAGGCTGGGAAAGTGAGCATCACTGGGAGCTGGGGAG CGGGGATGACCCTTGCCCCTGAACTTTGTCAGACCCTTCCACAGGGTCACTCACAGCCCCAGCCCACCTG ACAAGGGCAAGGGTTCTTGCATGTGGGGTACACACCCTCCCGACAAACAGAGTGGGAGCTGGGGGCTCTT ATTGGATGAGGTTGTGGGCCCAGCCACATGCAGTTCCCGTCCAGTAAGTGCTTTGCCTGCTGTGGGAGAG GGACTGGATCCCGTCCTCTAACGTCTGTCTCTGGCCGAGTCTAACACTGTACAACTGTCTCTGACCATTA AATGCTGTTGTACCGTGGAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_027151
• Insert Size: 1209 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC004613, AAH04613
• RefSeq Size: 1653 bp
• RefSeq ORF: 1209 bp
• Locus ID: 69654
• UniProt ID: Q99KJ8
• Cytogenetics: 10 74.5 cM
• Summary: Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (3) lacks two in-frame exons in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.