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Frmd8 (NM_026169) Mouse Untagged Clone

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SKU
MC200042
Frmd8 (untagged) - Mouse FERM domain containing 8 (Frmd8), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Frmd8
Synonyms 1200004M23Rik; 2310035N23Rik; 4931429L16Rik; AU018809
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC004765 sequence for NM_026169
CCCACGCGTCCGGCGGGGCCTCGGGTTTGTGCTTCCTCCCGCGGCAGCTTCTGCGGCGTAACGCCTGCGA ATCGACCCTGGGCAGGACCGGAGACCCCGCCGGCCGGTGTGGTGGTGGGTCCAGAACTGGAGGCCTGAGT CGGGTGGCATTCTGGAGTGAGGCAGTGGGCTCTGCGACCCTGCAAGATGGAGGGGGCAGAAGGCAACGCG GGGCAGCCCGGCCCTGCGGAGCGGTCCCACCGAAGTAGCGTCTCTTCAGTGGGTGCCCGAGCAGCCGATG TCCTGGTGTACCTGGCGGATGATACAGTAGTGCCCCTGGCCGTGGAGAATTTGTCCTCAATCAGTGCCCA TGAGCTGCACCGCGCAGTCCGTGAAGTCCTGCAGCTCCCAGACGTGGCTTTGGAGGCCTTTGCACTCTGG CTCGTCTCCCCTCTCTTGGAGGTGCAATTGAAACCCAAGCACCAGCCCTATAAACTGGGCCGCCAGTGGC CGGAGCTGCTGCTGCGCTTCACCGACGCCTCCGATGATGATGTCGCCATGGATGAGCCTTCCCTTCAGTT CCGAAGGAATGTTTTTTTCCCAAGGCGCCGAGAGCTCCAGATCCATGATGAGGAGGTCTTGCGGCTGCTC TATGAGGAAGCCAAGGGCAATGTGCTGACCGCTCGGTACCCTTGTGACTTGGAGGACTGCGAGGTGCTGG GTGGCCTTGTGTGCCGTGTGCAGCTTGGGCCCTACCAGCCTGGCCAGCCTGCCGCCTGCACTCTGAGAGA GAAGCTGGACTCCTTCCTCCCTGCCCACCTCTGCAAGCGAGGGCATGGCCTCTTTGCTGCCTTCCGGGGC CGTGGGGCCAAGACTGGGCCAGGTGAGCAAGGCCTGCTGAATGCCTACCGCCAGGTAAAGGAAGTGACAG GCAACAACAGTGAACGTGAGGCCACCTTGGGCTCCCACTACCGCGCATACCTCCTCAAGTGCCACGAGCT GCCCTTCTACGGGTGTGCCTTCTTCCATGGCGAGGTTGACAAGCCAGCCCAGGGCTTCTTGCATCGAGGT GGGCGCAAACCAGTGACTGTGGCCATCAGTCTGGAGGGTGTGCACGTCATCGACAACAGGGAGAAGCATG TGCTGCTGGGCCTGCGCTTTCAGGAGCTGTCGTGGGATCACACTTCCCCTGAGGAGGAAGAGCCTGTCCT GTGGCTGGAGTTCGATGGTGACAGTGAGGGCACGCCTGTCAACAAGCTGCTAAGGATCTACTCCAAGCAG GCTGAGCTGATGAGCGGCCTCATTGAGTACTGCATTGAGCTGAGCCAGGCTGCCGAGCCTACTCTTTCCC AGGAGAGTGCATCCGGCCCTCACGAAGCTCCCAGCCCCTCTCCACCTCCCACCCAGCGCCCCAAGCTGCG AAGGCAGGGTAGCGTGGTGTGTAGCCGGATTCAGCATCTCTCTACCATTGACTATGTGGAAGACGGCAAG GGGATCAAGCGGGTGAAACCAAAGCGAACCACCTCCTTCTTCAGCCGACAGCTCTCCTCCAGCCAGGGAA GCTACACCGTGGTGCAGCCCACCGATGACAGCCTGGAGCAGAGCTGAGGGAAGCCACGGCCAGCAGCAGG AGGACATCTGGGTGCCCTAGACACAACCAGGCACAGCCCTAGGCAGGGCCAGGCCACACTCGTCTCAGAT GCTGTGGGCCTGGGTGGGGTGCTGTGCAGGGTCTGCTGGAGTTTCAGACCATGGAAAACTGACATCCAAG CCTTCCCTGAGCCTGGTGTGGGTAGAGCTGTGCTCCAGCCCTTTCTACAGCCTGCCTCTTCCTCTGCTCC AGGCTGGCAGCTGACAAACCCTGCTGCCCTCCTGCTCTTCCCCAAGTGCACTGGTCTTCCCTCTGGAGGG ACTGCTGGCGGTTCCGATGGCAGGTGACTGAAGGCAGAGCTTGGGACCTGCAAGTTCAGTGTTTGCCTTC GGTCATGGGCCCTTGGTGACTTTATCTTGCATTCCTTCTGCTGACATTCCACCATGCTGGCCGGCCAGGC TGCCACCTTTTCCCCTAAGTTATGTTGGAACTGGAGGGCACCTCTGCAGGCTAACCCCCTTCTTGTTGGC CTTTACTGATGTCTCTGTGCTGAATGGTGGCTCTCCTAGGAACCGGTTCCATGCTGCACATAGGAAAAGC CCTTACATGTCCCTGGGCTGGATTGGCTGTCCCATACCAACCAGTTCCTTTGTTTTCCTGTGATCTGGGT TTAAGGGTTAGATTTTGCCTTGTCTCCTGTGCTCTGAGCTGATAAAGCCTGGGGCGTGGCAACTCCGAAT TGCACTGCACTGTGGGATTCCCTGAGGCCAGCCACAGCGGCTCAAGCCACCTCTCTCCGAAGGACATCGC AAAGGCCTATCTGTCCCCAGCTCTGCACCCCTGGCAGCCTTGACTGCTCCTGGGAAGAGCTAGTTCTGGG AGTGGAGCTGTCCTGGCCCTTAAGCCCTTTGTACTCCCTGGGAATCCCCACTGGATCCCAGAGACATAGC TTAGCTTTGTCTTCTTGGTGGAAAGCCACAGAAATGGAAACCATCATCTCTGAAAATGAAACCAGTTTGC CTGCCAGTGAGCTCCAGAAGTTTTGGCAGCAGAGAGAAAGAAAAGGCTGACCTCACTATAAGGCATTGTC TGTAAGATGATGTCACCTGCCACTGTAGTTAACCCTTTAGGAGCCTTGTGTGTTCCCTGAGCTACCAGCC CACTCTTGGCAACCTTTAGGTCATCTTGACTGCTGTTGATGGCTTCAGATCATTATGTGAGGGGCCCAGA TGCGTGTGCACAGGTACACAAACTCTACATTTCCATTTGAGCCTTTGAGTTTTTTAATTACCACATGTGC CTTAACCCTCTAGGACAGAGCACTGTTTTACTAAATAAATGTCTTGAGTGTTCCCAGTCTTTCCTATCAC TGGACTTAATAAAATCATCACCTAGCAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_026169
Insert Size 1401 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC004765, AAH04765
RefSeq Size 2982 bp
RefSeq ORF 1401 bp
Locus ID 67457
UniProt ID Q3UFK8
Cytogenetics 19 A
Summary Promotes the cell surface stability of iRhom1/RHBDF1 and iRhom2/RHBDF2 and prevents their degradation via the endolysosomal pathway (PubMed:29897333). By acting on iRhoms, involved in ADAM17-mediated shedding of TNF, amphiregulin/AREG, HBEGF and TGFA from the cell surface (By similarity). Negatively regulates Wnt signaling, possibly by antagonizing the recruitment of AXIN1 to LRP6 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026169" in other vectors (4)
SKU Description Size Price
MG207454 Frmd8 (tGFP-tagged) - Mouse RIKEN cDNA 1200004M23 gene (1200004M23Rik) 10 ug
$657.00
MR207454 Frmd8 (Myc-DDK-tagged) - Mouse FERM domain containing 8 (Frmd8) 10 ug
$289.00 $457.00
MR207454L3 Lenti ORF clone of Frmd8 (Myc-DDK-tagged) - Mouse FERM domain containing 8 (Frmd8) 10 ug
$757.00
MR207454L4 Lenti ORF clone of Frmd8 (mGFP-tagged) - Mouse FERM domain containing 8 (Frmd8) 10 ug
$757.00

file-alt Citations

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